FastQCFastQC Report
Thu 26 May 2016
SRR1512254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245604
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70890.31568344196038123No Hit
GTCCTACAGTGGACATTTCTAAATT56370.25102377801250797No Hit
CTGTAGGACGTGGAATATGGCAAGA54380.24216201966152537No Hit
GTCCTAAAGTGTGTATTTCTCATTT50030.22279083934656332No Hit
CTTTAGGACGTGAAATATGGCGAGG46900.20885249580959064No Hit
TATCAACGCAGAGTACTTTTTTTTT45720.20359778482760094No Hit
GGTATCAACGCAGAGTACTTTTTTT44290.1972297876206134No Hit
GTCCTACAGTGTGCATTTCTCATTT33710.15011551457870576No Hit
CTGAAGGACCTGGAATATGGCGAGA25250.11244190872477962No Hit
CTGTAGGACCTGGAATATGGCGAGA24450.1088793928047866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12250.013.8176941
ATTAGAC1155.304173E-912.3952293
TAGGACC10300.012.2708764
GGCGAGG12800.012.016819
GTCCTAT3450.011.5766221
CCAACGA1157.132985E-811.55883219
TAGAAAT9050.011.2356024
AATGTCC8950.010.9376438
AAATGTC9400.010.818467
CCTACAC2300.010.7425333
ATTTAGA8600.010.61510851
AGGACCT19300.010.5862535
GGACGTT909.4772506E-510.5600766
GTCCTAA16250.010.5334531
GTATCAA26800.010.46740151
TGTAGGA32850.010.4142572
CACCTTT7900.010.33698514
TTAATAC1856.002665E-1110.2735233
ATTTCTA21900.010.23201815
TCCAACG2053.6379788E-1210.18926718
Produced by FastQC (version 0.11.5)