FastQCFastQC Report
Thu 26 May 2016
SRR1512253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038978
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68600.33644306118065026No Hit
GTCCTACAGTGGACATTTCTAAATT43240.21206702573544198No Hit
GGTATCAACGCAGAGTACTTTTTTT42680.20932055176662032No Hit
GTCCTAAAGTGTGTATTTCTCATTT41410.203091941158757No Hit
TATCAACGCAGAGTACTTTTTTTTT39880.19558818192251218No Hit
CTGTAGGACGTGGAATATGGCAAGA39870.1955391377444975No Hit
CTTTAGGACGTGAAATATGGCGAGG36360.1783246312613476No Hit
GTCCTACAGTGTGCATTTCTCATTT22900.11231116765359903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT250.006089989618.9689318
TATACCG400.00527593614.2515895
GGTATCA12400.014.0979981
CGAATGG655.427011E-513.16082619
GGCGAGG9850.013.12371219
TAGGACC8100.012.1988924
CGTGCGC700.00150296310.84739410
TTAGGAC15400.010.7971913
TACTATA2300.010.7408572
GTCCTAC20850.010.70836451
CCGAATG803.765289E-410.68948218
TGGCGAG22900.010.580564518
GTACTAG909.5090654E-510.5564731
TCGAACT1002.3853587E-510.45554419
GACGTGA13200.010.2928157
TCCTACA24000.010.2141422
TTTAGGA17700.010.199382
GTGGTAT3850.010.1177631
GTGTAGG3200.010.0946261
AGGACGT25650.010.0751985
Produced by FastQC (version 0.11.5)