FastQCFastQC Report
Thu 26 May 2016
SRR1512253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038978
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80880.39666931178266757No Hit
GGTATCAACGCAGAGTACTTTTTTT51190.25105714725710626No Hit
TATCAACGCAGAGTACTTTTTTTTT49620.24335721130880275No Hit
CTGTAGGACGTGGAATATGGCAAGA43910.21535298566242497No Hit
GTCCTACAGTGGACATTTCTAAATT42720.20951672847867903No Hit
GTCCTAAAGTGTGTATTTCTCATTT42520.20853584491838556No Hit
CTTTAGGACGTGAAATATGGCGAGG39090.19171369185935308No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23760.11652896696286082No Hit
GTCCTACAGTGTGCATTTCTCATTT21860.10721057314007311No Hit
CTGAAGGACCTGGAATATGGCGAGA20800.10201189027051788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT402.752218E-416.6324547
GGTATCA12150.013.9311181
TCCAACG1650.013.81047618
TCGAGAC500.00149753613.3026983
AGGGTCG604.082618E-412.6704795
TAGGACC7500.012.54285054
GGCGAGG11800.011.18416319
CTACTAG951.3461975E-511.010611
GCCGGTT1303.26836E-810.96134111
GTATCAA28100.010.9304611
ACCGTCC700.001486179410.8620118
CCAACGA1502.1536835E-910.76039919
GTATTAG1605.347829E-1010.6978071
TGTAGGA24200.010.6795892
GTATTGG2050.010.6688161
TGGACTG3500.010.5889015
AGTACCT1355.664333E-810.5587335
ACTAGAC1355.6723366E-810.5576963
GTCCTAC23250.010.5521031
TGGCGAA909.571467E-510.54966918
Produced by FastQC (version 0.11.5)