FastQCFastQC Report
Thu 26 May 2016
SRR1512252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919780
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79680.41504755753263395No Hit
GTCCTACAGTGGACATTTCTAAATT66630.34707101855420935No Hit
CTGTAGGACGTGGAATATGGCAAGA60300.314098490452031No Hit
GTCCTAAAGTGTGTATTTCTCATTT56520.29440873433414244No Hit
GGTATCAACGCAGAGTACTTTTTTT49050.25549802581545805No Hit
TATCAACGCAGAGTACTTTTTTTTT47860.24929939888945607No Hit
CTTTAGGACGTGAAATATGGCGAGG47330.24653866588879975No Hit
GTCCTACAGTGTGCATTTCTCATTT35900.18700059381804165No Hit
CTGTAGGACCTGGAATATGGCGAGA28470.14829824250695392No Hit
CTGAAGGACCTGGAATATGGCGAGA27270.14204752627905282No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23410.1219410557459709No Hit
GTCCTTCAGTGTGCATTTCTCATTT22720.11834689391492775No Hit
ATTTAGAAATGTCCACTGTAGGACG21350.11121065955474063No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20440.10647053308191563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12100.014.1336331
TCCAACG1650.013.246510518
GTCTAGG1650.013.2437371
GTGTAGG4300.012.8152251
CACCGTT604.0938833E-412.66626557
GCCGGTT1207.4942363E-1012.65831811
TAGGACC11000.012.52331354
ACCGTGC701.1089257E-412.1931648
CGAAATC1552.5465852E-1111.6472313
GCGACTA1406.693881E-1011.54682716
GTCCTAG3250.011.4011311
CCGGTTT1105.0005656E-711.22192612
TGTAGGA35850.010.9715122
GTATTAG1701.382432E-1010.61871
GTCCTAC36450.010.6087341
CTGTCCG909.6494E-510.5411579
CCTACAC3250.010.5238463
CTGTAGG33600.010.51891
GTGGTAT3800.010.5010411
CGACTAA1451.4204488E-810.49368317
Produced by FastQC (version 0.11.5)