FastQCFastQC Report
Thu 26 May 2016
SRR1512252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919780
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT95510.4975049224390295No Hit
GTCCTACAGTGGACATTTCTAAATT66210.34488326787444396No Hit
CTGTAGGACGTGGAATATGGCAAGA62490.3255060475679505No Hit
TATCAACGCAGAGTACTTTTTTTTT61160.3185781704153601No Hit
GGTATCAACGCAGAGTACTTTTTTT60290.3140464011501318No Hit
GTCCTAAAGTGTGTATTTCTCATTT55740.29034576878600676No Hit
CTTTAGGACGTGAAATATGGCGAGG51320.267322297346571No Hit
GTCCTACAGTGTGCATTTCTCATTT36730.19132400587567328No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29840.155434476867141No Hit
CTGTAGGACCTGGAATATGGCGAGA29620.15428851222535914No Hit
CTGAAGGACCTGGAATATGGCGAGA28940.1507464396962152No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21500.11199199908322828No Hit
ATTTAGAAATGTCCACTGTAGGACG21170.11027305212055548No Hit
TTTCTAAATTTTCCACCTTTTTCAG20990.10933544468637031No Hit
GTCCTTCAGTGTGCATTTCTCATTT20010.10423069310025107No Hit
GAATATGGCAAGAAAACTGAAAATC20000.1041786037983519No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19650.10235547823188072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.014.3107981
TAGGACC11900.013.4163664
GACGTTA604.0755974E-412.6729947
GGCGAGG13700.012.54428119
TCCAACG1750.011.93575118
ACTGTTC5950.011.6612848
TGTAGGA34650.011.6285042
CTGTAGG34150.011.3896671
TGTCGAG1604.5474735E-1111.27453818
CGTTAAA600.005858116311.0880029
GTAGGAC34950.011.0120733
GTGGTAT4500.010.9892791
ACGTTAA700.001485476210.8625668
GACCGTG700.001485476210.8625667
AGGACCT23650.010.7693015
GGACCTG23250.010.7513256
TGGCGAG33000.010.7314918
GGTCTAA803.735223E-410.6986971
AAGTCGT2400.010.6928397
TATGTCG1605.493348E-1010.68086216
Produced by FastQC (version 0.11.5)