##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512252_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1919780 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.882688641406826 33.0 31.0 34.0 30.0 34.0 2 32.06250351602788 34.0 31.0 34.0 30.0 34.0 3 32.15330089906135 34.0 31.0 34.0 30.0 34.0 4 35.510324099636414 37.0 35.0 37.0 33.0 37.0 5 35.35639448270114 37.0 35.0 37.0 33.0 37.0 6 35.40675389888425 37.0 35.0 37.0 33.0 37.0 7 35.32847565866922 37.0 35.0 37.0 33.0 37.0 8 35.293961808123846 37.0 35.0 37.0 33.0 37.0 9 36.94197303857734 39.0 38.0 39.0 34.0 39.0 10 36.85288053839503 39.0 37.0 39.0 33.0 39.0 11 36.9084421131588 39.0 37.0 39.0 34.0 39.0 12 36.8228661617477 39.0 37.0 39.0 33.0 39.0 13 36.80793059621415 39.0 37.0 39.0 33.0 39.0 14 38.13467532738126 40.0 38.0 41.0 33.0 41.0 15 38.12252289324818 40.0 38.0 41.0 33.0 41.0 16 38.082260467345215 40.0 38.0 41.0 33.0 41.0 17 38.06211388804967 40.0 38.0 41.0 33.0 41.0 18 38.02379178864245 40.0 38.0 41.0 33.0 41.0 19 38.05478752773755 40.0 38.0 41.0 34.0 41.0 20 38.0166784735751 40.0 38.0 41.0 33.0 41.0 21 37.9554167665045 40.0 38.0 41.0 33.0 41.0 22 37.894892643948786 40.0 38.0 41.0 33.0 41.0 23 37.82959870401817 40.0 38.0 41.0 33.0 41.0 24 37.78797935180073 40.0 38.0 41.0 33.0 41.0 25 37.71066893081499 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3062990217568107 1101 2 0.35286341457142356 1101 3 0.3122206069326765 1101 4 0.3542489419948396 1101 5 0.29035700575591505 1101 6 0.35703551760320096 1101 7 0.45614596240960736 1101 8 0.45916780347057795 1101 9 0.3726845564415697 1101 10 0.5327399974433717 1101 11 0.4260417177747513 1101 12 0.42805634717792884 1101 13 0.40971631162452127 1101 14 0.6119634154272973 1101 15 0.6164666736948803 1101 16 0.505633509226648 1101 17 0.5761082299328137 1101 18 0.6259171043187237 1101 19 0.5835791000227175 1101 20 0.5521376411585166 1101 21 0.5307411497111758 1101 22 0.5195691586112616 1101 23 0.6629214133304586 1101 24 0.7099575879689013 1101 25 0.6911246441283012 1102 1 0.37666877633808227 1102 2 0.37626891570996435 1102 3 0.3910611028303137 1102 4 0.45951514360687185 1102 5 0.3181720923211415 1102 6 0.4018701581496984 1102 7 0.4794068869767827 1102 8 0.4497891212860239 1102 9 0.47663655701987295 1102 10 0.4909111038155842 1102 11 0.5407935882341448 1102 12 0.5022891877461149 1102 13 0.5930379583516654 1102 14 0.7005758417836034 1102 15 0.7097055503349239 1102 16 0.6558095312022871 1102 17 0.6822650232244598 1102 18 0.806806973115485 1102 19 0.7126512798759777 1102 20 0.6655348291441996 1102 21 0.7215402967093993 1102 22 0.694956443101816 1102 23 0.7147506683996738 1102 24 0.679548797713359 1102 25 0.8457666363934564 1103 1 0.25769809033607416 1103 2 0.26916729596955946 1103 3 0.270567012208339 1103 4 0.3510930187186503 1103 5 0.29195549022387013 1103 6 0.27152162163815774 1103 7 0.3978407463099458 1103 8 0.46350142144455475 1103 9 0.4372140741550652 1103 10 0.5169626773231073 1103 11 0.5692296451842083 1103 12 0.4556129105367859 1103 13 0.5641811752481587 1103 14 0.6056274581377465 1103 15 0.5100367132336245 1103 16 0.5117625160221451 1103 17 0.5890996117794316 1103 18 0.6328021549441019 1103 19 0.5956849863434641 1103 20 0.6156605355951044 1103 21 0.5695453719605013 1103 22 0.6708686268833972 1103 23 0.6570857538372152 1103 24 0.5625769755848822 1103 25 0.5789485783404373 1104 1 0.41847002573610226 1104 2 0.40297293318254646 1104 3 0.3809560367315967 1104 4 0.3906612390684785 1104 5 0.4154040177642315 1104 6 0.4341225338373107 1104 7 0.4861829381464773 1104 8 0.4648768572438584 1104 9 0.5654035056263425 1104 10 0.5886937953797755 1104 11 0.5572541697943265 1104 12 0.5525554139807554 1104 13 0.47851794559993976 1104 14 0.5346256684821995 1104 15 0.5099850030958777 1104 16 0.6361515216927529 1104 17 0.6438280594922716 1104 18 0.602445610851106 1104 19 0.5937224250218378 1104 20 0.6884323201739022 1104 21 0.7172833900886815 1104 22 0.748383469982187 1104 23 0.7132774368540709 1104 24 0.7185880771050748 1104 25 0.6520176962112672 1105 1 0.33362255748619063 1105 2 0.3232494290807466 1105 3 0.3177211231430661 1105 4 0.38573153981497654 1105 5 0.3253296015666436 1105 6 0.33696304027834856 1105 7 0.3572087322074893 1105 8 0.3914213216695899 1105 9 0.48136534440728695 1105 10 0.43332244906164163 1105 11 0.4829615007585133 1105 12 0.38043749753026646 1105 13 0.465240317140001 1105 14 0.5656300335909705 1105 15 0.6124528434137773 1105 16 0.4897270229911044 1105 17 0.5828385048061833 1105 18 0.5845956057149166 1105 19 0.6205322807795071 1105 20 0.5578961932749777 1105 21 0.6712121830327291 1105 22 0.7305566101948884 1105 23 0.6786660381598324 1105 24 0.5977316471841192 1105 25 0.535731042365569 1106 1 0.37103031242351747 1106 2 0.3384693263887044 1106 3 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0.6681624696325343 1107 16 0.7426944119354246 1107 17 0.6662823540186196 1107 18 0.7451166873330735 1107 19 0.7927635347302626 1107 20 0.6978405714600129 1107 21 0.757539970423629 1107 22 0.712601914788678 1107 23 0.6814372050330917 1107 24 0.6932540549928277 1107 25 0.6799673460650055 1108 1 0.1754227486626796 1108 2 0.161047179361276 1108 3 0.18771351054812868 1108 4 0.28374354764449805 1108 5 0.16362024456229562 1108 6 0.018411420093109143 1108 7 0.006221730383913382 1108 8 0.124096839625075 1108 9 0.11834821252084993 1108 10 0.11616081167457537 1108 11 0.1417715335707257 1108 12 0.05698406562852654 1108 13 0.14217739818119668 1108 14 0.16379145506822113 1108 15 0.16973457936924774 1108 16 0.19425925386661191 1108 17 0.24273847672777293 1108 18 0.3414585982655254 1108 19 0.2580222328637447 1108 20 0.3791330017427015 1108 21 0.3742031421892804 1108 22 0.4455523578865268 1108 23 0.3814299372275656 1108 24 0.32250542336571897 1108 25 0.29803105969917 1109 1 -0.012289702334836505 1109 2 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1110 13 0.14474613826116922 1110 14 0.14730537241079844 1110 15 0.15301823116680424 1110 16 0.1645205750233174 1110 17 0.15822776542587036 1110 18 0.08896056598927515 1110 19 0.1723355391420398 1110 20 0.11452021070100216 1110 21 0.10968850474354497 1110 22 0.16700966432856745 1110 23 0.08730268550964837 1110 24 0.09271715602323383 1110 25 0.09619377324138867 1111 1 -0.266262156746091 1111 2 -0.222217954909123 1111 3 -0.2881218269486432 1111 4 -0.2522567917966043 1111 5 -0.2901528204136312 1111 6 -0.26528999856552105 1111 7 -0.25928485281505687 1111 8 -0.2679233571202033 1111 9 -0.4947591096152806 1111 10 -0.35114023994005095 1111 11 -0.35768923067531233 1111 12 -0.3770125921822114 1111 13 -0.2871139439260091 1111 14 -0.35264299596506987 1111 15 -0.2990235511309365 1111 16 -0.26848685258547533 1111 17 -0.23020601626006965 1111 18 -0.2792297304626601 1111 19 -0.1765103968442503 1111 20 -0.2703604943234197 1111 21 -0.1887904578539903 1111 22 -0.20173453791531415 1111 23 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-0.7485193974089839 2314 10 -0.7461310574464974 2314 11 -0.7282169352462589 2314 12 -0.7936771175266486 2314 13 -0.8107000504515725 2314 14 -1.1089926281794789 2314 15 -1.0619759414403518 2314 16 -1.0931561802498848 2314 17 -1.1467611722220994 2314 18 -1.2015971187369274 2314 19 -1.0848644686134534 2314 20 -0.9379987094755649 2314 21 -0.9831717003031315 2314 22 -1.1434839513909623 2314 23 -1.072766855429677 2314 24 -0.9100366910254749 2314 25 -0.8591877863455863 2315 1 -0.4141278258405592 2315 2 -0.484631324982832 2315 3 -0.4269319245226413 2315 4 -0.3418014294570426 2315 5 -0.3665906474411429 2315 6 -0.596725593746477 2315 7 -0.703791556377702 2315 8 -0.7406176991703859 2315 9 -0.8919592362324522 2315 10 -0.8257810658108014 2315 11 -0.7319932373504301 2315 12 -0.7330442668985953 2315 13 -0.7446118543049707 2315 14 -0.8500426394531075 2315 15 -0.8228249911825145 2315 16 -0.8279465998470883 2315 17 -0.8115849035751523 2315 18 -0.8293076992197257 2315 19 -0.6986968479310747 2315 20 -1.5171226757565037 2315 21 -1.1627524303979015 2315 22 -1.0736914589006545 2315 23 -0.8790157189751184 2315 24 -0.8508357731338592 2315 25 -0.9195624344992552 2316 1 -0.9200228744447365 2316 2 -0.758052060127163 2316 3 -0.7234297538987491 2316 4 -0.49276609624897105 2316 5 -0.6329444937471251 2316 6 -0.6682758345423423 2316 7 -0.6996703376638536 2316 8 -0.7117048900171739 2316 9 -0.7897297731033959 2316 10 -0.7713832038011716 2316 11 -0.9175964136802435 2316 12 -0.9293893120416428 2316 13 -0.9281470045675206 2316 14 -0.9966524611553922 2316 15 -1.0442817814280616 2316 16 -1.08038546143149 2316 17 -1.1785006893014511 2316 18 -1.2738121640793523 2316 19 -1.2502269152748013 2316 20 -1.2912764291063823 2316 21 -1.2536590490702082 2316 22 -1.320154924185502 2316 23 -1.3357441585981533 2316 24 -1.3469494169142067 2316 25 -1.316130228176263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17542.0 3 4.0 4 513.0 5 2572.0 6 3325.0 7 2560.0 8 2111.0 9 1938.0 10 1746.0 11 1666.0 12 1625.0 13 1544.0 14 1437.0 15 1478.0 16 1490.0 17 1609.0 18 1810.0 19 2031.0 20 2566.0 21 3171.0 22 4241.0 23 5162.0 24 6203.0 25 7178.0 26 8324.0 27 9585.0 28 10916.0 29 13456.0 30 17038.0 31 22483.0 32 29837.0 33 39856.0 34 54944.0 35 76797.0 36 119558.0 37 271045.0 38 1170419.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.91681306012117 19.946792562430353 13.868192182928462 24.268202194520008 2 14.620835577521104 20.590340321308652 42.36868506397107 22.42013903719917 3 19.083645000989698 25.244090468699536 30.121159716217484 25.551104814093286 4 12.524073637068792 17.385789248037774 38.68936269522632 31.400774419667115 5 12.618383646383046 38.50818889694787 36.009314375276155 12.864113081392926 6 31.181143391615407 37.942541223428506 18.082397355030835 12.793918029925253 7 27.675535784639692 31.717554308060798 22.44057433862048 18.166335568679035 8 24.293738784075174 38.697553253979876 20.400773693584192 16.607934268360758 9 26.204028237795807 15.099246627282465 20.716681303274864 37.98004383164687 10 15.117707683121392 29.24143127040515 34.684402103742826 20.956458942730634 11 33.84474305334841 22.005502091941207 25.819660600124006 18.330094254586378 12 23.961246556251282 24.544455760011758 32.34794083892481 19.14635684481215 13 29.356021913787423 22.44318300263916 26.043208435307214 22.1575866482662 14 20.06348670483371 23.431470462740243 27.971035558493885 28.53400727393216 15 22.045076621087155 33.16419876055962 23.42246412749482 21.368260490858397 16 20.821471825391658 28.393337587414862 29.65763325476305 21.127557332430428 17 20.67264064744866 29.65517816194343 28.746162076786597 20.926019113821315 18 20.471431510898924 27.177688006105168 32.314832902273025 20.03604758072288 19 23.196332760327135 26.692295671198625 28.534093868833672 21.577277699640568 20 23.37792089878697 27.314593672173825 29.48774914142153 19.819736287617665 21 22.01832329552617 26.8032371016522 28.10424525795654 23.07419434486509 22 22.72801859060812 29.014955104473195 28.328850813078642 19.92817549184004 23 21.09575268859214 28.59531641910001 29.38815482748472 20.920776064823134 24 22.906023511442903 26.676428806961226 30.10146062247449 20.316087059121376 25 21.76538611798411 27.96410991014455 29.648027086860267 20.622476885011068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 176.0 1 176.0 2 424.0 3 672.0 4 672.0 5 672.0 6 1888.0 7 3104.0 8 3104.0 9 3104.0 10 4231.0 11 5358.0 12 5358.0 13 5358.0 14 7745.0 15 10132.0 16 10132.0 17 10132.0 18 17498.0 19 24864.0 20 24864.0 21 24864.0 22 39993.5 23 55123.0 24 55123.0 25 55123.0 26 80834.0 27 106545.0 28 106545.0 29 106545.0 30 138021.0 31 169497.0 32 169497.0 33 169497.0 34 186545.5 35 203594.0 36 203594.0 37 203594.0 38 212208.5 39 220823.0 40 220823.0 41 220823.0 42 230350.0 43 239877.0 44 239877.0 45 239877.0 46 243893.0 47 247909.0 48 247909.0 49 247909.0 50 234863.5 51 221818.0 52 221818.0 53 221818.0 54 191861.5 55 161905.0 56 161905.0 57 161905.0 58 137598.0 59 113291.0 60 113291.0 61 113291.0 62 92317.0 63 71343.0 64 71343.0 65 71343.0 66 54376.0 67 37409.0 68 37409.0 69 37409.0 70 27134.0 71 16859.0 72 16859.0 73 16859.0 74 11624.0 75 6389.0 76 6389.0 77 6389.0 78 4273.0 79 2157.0 80 2157.0 81 2157.0 82 1402.5 83 648.0 84 648.0 85 648.0 86 419.5 87 191.0 88 191.0 89 191.0 90 131.5 91 72.0 92 72.0 93 72.0 94 45.5 95 19.0 96 19.0 97 19.0 98 12.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10433487170404943 2 3.1253581139505565E-4 3 0.0 4 0.007761305982977217 5 0.031045223931908866 6 0.034639385762952 7 0.04750544333204846 8 0.06203835856191855 9 0.05599599954161414 10 0.027815687214159956 11 0.02656554396857973 12 0.05656898186250507 13 0.05146423027638583 14 0.016824844513433832 15 0.010626217587431893 16 0.002864911604454677 17 0.006354894831699465 18 0.004948483680421715 19 0.004167144151934076 20 0.00192730417026951 21 0.001979393472168686 22 0.005261019491816771 23 0.004948483680421715 24 0.006198626926001938 25 0.0015626790569752786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1919780.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.27537498916946 #Duplication Level Percentage of deduplicated Percentage of total 1 83.8198704174718 56.39013213879009 2 11.032637318915182 14.844496254990483 3 2.6952951710305406 5.439809800127318 4 0.9345260961701244 2.5148237422803907 5 0.452883642772404 1.5233958446987277 6 0.2533523475550218 1.0226624511690292 7 0.1551689404808917 0.7307334060266875 8 0.11549637203312683 0.6216049390733785 9 0.07538863817768693 0.45646190129940356 >10 0.40368104222483364 4.926231942507283 >50 0.0293083996291259 1.3830653725998092 >100 0.026658724215485225 3.7350833907070937 >500 0.002866444661854289 1.413473433395701 >1k 0.002324144320422396 2.6384837423386416 >5k 5.423003414318924E-4 2.35954163999595 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9551 0.4975049224390295 No Hit GTCCTACAGTGGACATTTCTAAATT 6621 0.34488326787444396 No Hit CTGTAGGACGTGGAATATGGCAAGA 6249 0.3255060475679505 No Hit TATCAACGCAGAGTACTTTTTTTTT 6116 0.3185781704153601 No Hit GGTATCAACGCAGAGTACTTTTTTT 6029 0.3140464011501318 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5574 0.29034576878600676 No Hit CTTTAGGACGTGAAATATGGCGAGG 5132 0.267322297346571 No Hit GTCCTACAGTGTGCATTTCTCATTT 3673 0.19132400587567328 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2984 0.155434476867141 No Hit CTGTAGGACCTGGAATATGGCGAGA 2962 0.15428851222535914 No Hit CTGAAGGACCTGGAATATGGCGAGA 2894 0.1507464396962152 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2150 0.11199199908322828 No Hit ATTTAGAAATGTCCACTGTAGGACG 2117 0.11027305212055548 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2099 0.10933544468637031 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2001 0.10423069310025107 No Hit GAATATGGCAAGAAAACTGAAAATC 2000 0.1041786037983519 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1965 0.10235547823188072 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 5.208930189917595E-5 13 0.0 0.0 0.0 0.0 2.083572075967038E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1555 0.0 14.310798 1 TAGGACC 1190 0.0 13.416366 4 GACGTTA 60 4.0755974E-4 12.672994 7 GGCGAGG 1370 0.0 12.544281 19 TCCAACG 175 0.0 11.935751 18 ACTGTTC 595 0.0 11.661284 8 TGTAGGA 3465 0.0 11.628504 2 CTGTAGG 3415 0.0 11.389667 1 TGTCGAG 160 4.5474735E-11 11.274538 18 CGTTAAA 60 0.0058581163 11.088002 9 GTAGGAC 3495 0.0 11.012073 3 GTGGTAT 450 0.0 10.989279 1 ACGTTAA 70 0.0014854762 10.862566 8 GACCGTG 70 0.0014854762 10.862566 7 AGGACCT 2365 0.0 10.769301 5 GGACCTG 2325 0.0 10.751325 6 TGGCGAG 3300 0.0 10.73149 18 GGTCTAA 80 3.735223E-4 10.698697 1 AAGTCGT 240 0.0 10.692839 7 TATGTCG 160 5.493348E-10 10.680862 16 >>END_MODULE