FastQCFastQC Report
Thu 26 May 2016
SRR1512251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151037
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7240.4793527413812509No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6810.4508828962439667No Hit
GGTATCAACGCAGAGTACTTTTTTT6430.42572349821566896No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT6390.4230751405284798No Hit
TATCAACGCAGAGTACTTTTTTTTT4270.2827121831074505No Hit
CTGTAGGACGTGGAATATGGCAAGA3340.22113786688030085No Hit
GTCCTACAGTGGACATTTCTAAATT3310.21915159861490893No Hit
GTCCTAAAGTGTGTATTTCTCATTT3310.21915159861490893No Hit
CTTTAGGACGTGAAATATGGCGAGG2800.18538503810324622No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2580.17081907082370545No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2110.13970086799923198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGA350.001268693617.6295531
TAGGACC509.314026E-515.06494
GTCCTAA902.0155858E-713.7118751
TACACTG500.00159221813.18178755
ACACTGT550.00325706311.9834446
AAAGTGT1105.448819E-711.1274846
TCCAGGA650.005862734311.0749752
GGTATCA1301.2514465E-711.0749751
CATATTC700.001592191410.7606443
CTAAAGT1159.666182E-710.643684
GGACCTG1002.6035159E-510.3571196
TCCTAAA1001.220353E-410.2839062
TAAAGTG1304.2291504E-710.1398365
AGGACCT857.0774014E-49.9694195
CCTAAAG1252.8157901E-69.7921853
ACTTTAG958.7317155E-49.7426482
GTCCTAC1801.1119482E-89.7125781
CACTTTA850.00383301789.678971
CCTACAG1951.886292E-99.1741383
TCCTACA1801.15634975E-79.141252
Produced by FastQC (version 0.11.5)