FastQCFastQC Report
Thu 26 May 2016
SRR1512251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151037
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8110.5369545210776168No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7900.5230506432198733No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT7760.513781391314711No Hit
GGTATCAACGCAGAGTACTTTTTTT6690.442937823182399No Hit
TATCAACGCAGAGTACTTTTTTTTT5730.37937723868985745No Hit
GTCCTACAGTGGACATTTCTAAATT3690.24431099664320666No Hit
CTGTAGGACGTGGAATATGGCAAGA3590.23769010242523356No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3440.22775876109827395No Hit
CTTTAGGACGTGAAATATGGCGAGG3360.22246204572389547No Hit
GTCCTAAAGTGTGTATTTCTCATTT3360.22246204572389547No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2130.14102504684282657No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1790.11851400650171812No Hit
GTGGTATCAACGCAGAGTACATGGG1610.10659639690936658No Hit
CTGAAGGACCTGGAATATGGCGAGA1560.10328594980038004No Hit
GTGGTATCAACGCAGAGTACTTTTT1540.10196177095678541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC350.002157514416.2862822
GGTATCA1950.014.674221
GTGGTAT400.005111712514.3073661
GGCGAGG903.567402E-813.71815719
ACCTTTT801.9721065E-613.0586315
CCTTTTT751.46222155E-512.66291416
CACCTTT853.8900525E-612.29047514
CTTTAGG1251.2787496E-912.2089531
TTTAGGA1156.8836016E-811.565622
GACGTGA855.265118E-511.173167
TTAGGAC909.380985E-510.5559233
TTCCACC909.4125884E-510.55242811
CCACCTT1002.3670087E-510.44690413
GTCCTAA951.557077E-410.04025651
CTGTAGG2201.8189894E-129.9718011
TGTAGGA2201.6370905E-119.5003322
TAGGACG2303.6379788E-129.5003314
ATGGCAA1701.6103513E-89.49718617
TCCACCT1201.67366E-59.49718512
GTCCTAC2053.7289283E-109.3056051
Produced by FastQC (version 0.11.5)