##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512251_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 151037 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.40072962254282 31.0 30.0 34.0 27.0 34.0 2 30.610876805021284 31.0 31.0 34.0 27.0 34.0 3 30.683137244516246 31.0 31.0 34.0 27.0 34.0 4 34.27115210180287 37.0 35.0 37.0 30.0 37.0 5 33.79317650641896 37.0 35.0 37.0 28.0 37.0 6 33.703847401630064 37.0 35.0 37.0 29.0 37.0 7 33.5117885021551 37.0 35.0 37.0 28.0 37.0 8 33.39810112753828 37.0 35.0 37.0 28.0 37.0 9 34.81517111701106 38.0 35.0 39.0 28.0 39.0 10 34.62898495070744 38.0 35.0 39.0 27.0 39.0 11 34.58361858352589 38.0 35.0 39.0 27.0 39.0 12 34.4247038805061 38.0 35.0 39.0 27.0 39.0 13 34.388825254738904 38.0 35.0 39.0 27.0 39.0 14 35.30724259618504 39.0 35.0 41.0 26.0 41.0 15 35.244635420459886 39.0 35.0 41.0 26.0 41.0 16 35.125068691777514 39.0 34.0 41.0 26.0 41.0 17 35.05893257943418 39.0 34.0 41.0 25.0 41.0 18 34.96681607817952 39.0 34.0 41.0 25.0 41.0 19 34.989466157299205 39.0 34.0 41.0 25.0 41.0 20 34.867423214179304 39.0 34.0 40.0 25.0 41.0 21 34.730867270933615 39.0 34.0 40.0 24.0 41.0 22 34.80329985367824 39.0 34.0 40.0 25.0 41.0 23 34.677853770930305 39.0 34.0 41.0 24.0 41.0 24 34.53292901739309 39.0 34.0 41.0 23.0 41.0 25 34.12784946734906 38.0 34.0 40.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09264968586894895 1101 2 0.10252907881350382 1101 3 0.10414176615342896 1101 4 0.2184572518714205 1101 5 -0.044562541233602815 1101 6 0.2889481510435914 1101 7 -0.02449429745158227 1101 8 -0.0890554200561553 1101 9 0.36931927357938577 1101 10 0.7253607501538895 1101 11 0.8904699186232747 1101 12 0.5928876783831143 1101 13 0.829855544923781 1101 14 0.30645721801947445 1101 15 0.3170879901526362 1101 16 0.1604146660047192 1101 17 0.42423595010430404 1101 18 0.7166259029500139 1101 19 0.2638937811066384 1101 20 0.17450499424064247 1101 21 0.26430966368844366 1101 22 0.2244289432140576 1101 23 0.3507170942758364 1101 24 -0.17661989331152483 1101 25 -0.38496343200009164 1102 1 -0.3703628641635248 1102 2 -0.08564921576013873 1102 3 -0.26698226485432386 1102 4 -0.17941096518284638 1102 5 -0.10386486681500173 1102 6 -0.30543169391765446 1102 7 -0.07526949125003313 1102 8 -0.4991329394359951 1102 9 -0.10296754812604547 1102 10 -0.29169351341200667 1102 11 0.13853193412715115 1102 12 0.520988453576912 1102 13 0.15970050616409281 1102 14 -0.17396913856967444 1102 15 0.14189419170302386 1102 16 0.006151100113250152 1102 17 0.3662902136701973 1102 18 0.41759489519807147 1102 19 0.1441263392461707 1102 20 -0.04991361041052045 1102 21 -0.17483762313325713 1102 22 0.09652196646987221 1102 23 -0.07815887471640792 1102 24 0.1731863082388685 1102 25 0.42705207187587746 1103 1 -0.029277592845694755 1103 2 0.05388566796079175 1103 3 0.29115727002939806 1103 4 0.5105115154373081 1103 5 0.3922591641927511 1103 6 0.46201016654746496 1103 7 0.6379088033236116 1103 8 0.08226240940120988 1103 9 0.49393167668015536 1103 10 1.0338878819368347 1103 11 0.22380325195661044 1103 12 1.0558721745071438 1103 13 0.29923538988501974 1103 14 0.08184481491870343 1103 15 0.6225143467417809 1103 16 0.5797945109659608 1103 17 0.15698788808879982 1103 18 0.5338739649655153 1103 19 0.4076922307190429 1103 20 -0.06541748637951628 1103 21 0.4840771055489128 1103 22 0.4763669277101812 1103 23 0.8288178694696384 1103 24 -0.051619893311524834 1103 25 0.1712381183875067 1104 1 1.176380164877319 1104 2 0.950912163390619 1104 3 1.5052611691385032 1104 4 1.1715667046077343 1104 5 1.627825518467887 1104 6 1.7960377032823942 1104 7 1.4334659015533902 1104 8 0.7835813958642461 1104 9 1.9398416616390861 1104 10 1.5646642327409523 1104 11 1.790967431061091 1104 12 1.4661463848507736 1104 13 1.3420445996501513 1104 14 1.673248262795596 1104 15 1.7628591344312454 1104 16 1.6599171535669086 1104 17 1.9278429152784327 1104 18 1.8898846094176704 1104 19 2.2313067164300193 1104 20 2.3757487753351683 1104 21 2.364558419907354 1104 22 1.4458219780399304 1104 23 1.5401449549723552 1104 24 2.2593253803203197 1104 25 2.817772886407873 1105 1 0.5879841169479505 1105 2 0.7237732102689023 1105 3 0.6094234562942731 1105 4 0.8028469232329201 1105 5 0.23219802214667595 1105 6 0.3125397003393644 1105 7 0.23912072602259826 1105 8 0.5339492747795305 1105 9 0.5724882876638873 1105 10 1.160102534191445 1105 11 0.7026764913932269 1105 12 0.9230519976319442 1105 13 0.4887757327172082 1105 14 1.0384994715406037 1105 15 0.4288250793545103 1105 16 0.5580672481643489 1105 17 0.49195895479913787 1105 18 1.1284803630439058 1105 19 0.8735181942521777 1105 20 1.359246778278198 1105 21 1.3626664712001855 1105 22 1.3112834033079537 1105 23 1.3529471412241918 1105 24 1.4507275245288511 1105 25 1.2828769435867642 1106 1 -0.21108034280325683 1106 2 0.1044147235823587 1106 3 0.3605286274672963 1106 4 0.16693657060621092 1106 5 0.3609119113211392 1106 6 0.1774518006786252 1106 7 0.10336458332700715 1106 8 0.6089981079243856 1106 9 0.7158521202947128 1106 10 0.3170882440711651 1106 11 0.0413215001317937 1106 12 0.48041809200842067 1106 13 0.1326535984517605 1106 14 0.6409462691143659 1106 15 0.21879115316966846 1106 16 0.19423461734292147 1106 17 0.334150791953455 1106 18 -0.12504076371665462 1106 19 -0.19243225295662114 1106 20 -0.3268332052727416 1106 21 0.1028741405740945 1106 22 -0.5772742198029732 1106 23 0.3770552937892191 1106 24 0.12112949841597498 1106 25 -0.5279074709295273 1107 1 -0.02431635253561737 1107 2 0.08086241214683554 1107 3 -0.33085823384656976 1107 4 -0.28654274812858205 1107 5 -1.0545625412336008 1107 6 -0.166051848956414 1107 7 -0.6411609641182494 1107 8 -0.30238875338948645 1107 9 -0.9490140597539494 1107 10 -0.961305916512778 1107 11 -0.44286341471005386 1107 12 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-0.17706862233592346 2315 3 -0.5926973143063421 2315 4 -0.20654274812858375 2315 5 -0.05548208146348799 2315 6 -0.17685644665755973 2315 7 -0.38254027446307504 2315 8 -0.10859564994120774 2315 9 -0.0418876229723395 2315 10 -0.3332599395012821 2315 11 -0.3394151388479898 2315 12 -0.42234220667435807 2315 13 -0.2603743401336871 2315 14 -0.17543933370465936 2315 15 0.0665132775089603 2315 16 -0.928091081121714 2315 17 -0.661108877481901 2315 18 -0.7284890395787258 2315 19 -1.0217384028014038 2315 20 -0.7030812126559098 2315 21 -0.6374144742425898 2315 22 -0.792812436096284 2315 23 -0.7926737103218642 2315 24 1.042446195143043E-4 2315 25 -0.04300940901158157 2316 1 -0.09056635253561751 2316 2 -0.059970921186497606 2316 3 -0.20835823384657104 2316 4 0.19970725187141625 2316 5 -0.23831254123360424 2316 6 -0.44230184895641145 2316 7 -0.1724109641182494 2316 8 -0.06926375338948532 2316 9 0.02348594024604722 2316 10 -0.4288059165127791 2316 11 -0.45328008137672526 2316 12 -0.4654456549502157 2316 13 -0.6211861217428876 2316 14 -0.17166778198052413 2316 15 -0.37978700984736236 2316 16 -0.3666686673286108 2316 17 -0.40805571656236594 2316 18 -0.3698324303833189 2316 19 -0.48402288556003015 2316 20 -0.10466167242602609 2316 21 -0.22110700297822206 2316 22 -0.3224460567859424 2316 23 -0.26699123905749644 2316 24 0.21400510668847517 2316 25 -0.10892176533342024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3343.0 3 0.0 4 52.0 5 540.0 6 661.0 7 555.0 8 395.0 9 373.0 10 335.0 11 359.0 12 294.0 13 331.0 14 295.0 15 275.0 16 266.0 17 310.0 18 328.0 19 409.0 20 443.0 21 540.0 22 742.0 23 945.0 24 1150.0 25 1291.0 26 1551.0 27 1756.0 28 1978.0 29 2454.0 30 3109.0 31 4007.0 32 4880.0 33 6432.0 34 8317.0 35 10799.0 36 15862.0 37 25462.0 38 50198.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.13123342130981 21.033247129684415 14.86181931803828 23.973700130967497 2 15.00029794023981 21.842992114514985 40.31727325092527 22.839436694319932 3 18.528572468997663 25.03426312757801 31.237378920396992 25.199785483027338 4 13.317928719452848 18.56564947661831 37.14586492051616 30.970556883412677 5 13.120161071336323 38.083569001715354 35.64233156057726 13.15393836637106 6 31.09502969471057 37.1617550666393 18.057826890099776 13.685388348550356 7 26.686838324384095 32.572151194740364 22.711653435913053 18.029357044962495 8 24.039804816038455 37.37560994987983 21.173619709077908 17.410965525003807 9 25.00314492475354 16.02786072286923 22.38789170865417 36.581102643723064 10 15.602799314075359 29.654323112879627 34.52134245251164 20.221535120533378 11 31.951111317094487 23.022173374735992 25.98833398438793 19.03838132378159 12 22.7745519309838 25.71820150029463 31.879605659540378 19.627640909181192 13 28.002410005495342 23.39625389805147 26.738481299284285 21.862854797168907 14 20.07852380542516 24.87999629229924 27.9626846401875 27.078795262088097 15 22.16410548408668 33.142210186907846 24.02325258049352 20.67043174851195 16 20.579063408303924 29.51793269198938 29.413322563345407 20.48968133636129 17 20.382422850030125 30.57595158802148 28.876368042267785 20.16525751968061 18 19.97258949793759 28.097750882234156 32.24971364632508 19.679945973503184 19 22.22369353204844 28.028231492945437 29.341816905791298 20.40625806921483 20 22.1588087687123 28.52281229102803 30.08865377357866 19.22972516668101 21 21.04252600356204 27.80907989433053 29.433185245999326 21.715208856108106 22 21.975409998874447 29.474234790150756 29.102802624522468 19.44755258645233 23 20.653217423545225 29.035269503499144 29.96749140938975 20.344021663565883 24 22.042281030475976 27.7918655693638 30.41042923257215 19.755424167588075 25 21.22193308304033 28.327473298769064 30.328493010998326 20.12210060719228 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1604.0 1 1604.0 2 935.0 3 266.0 4 266.0 5 266.0 6 327.5 7 389.0 8 389.0 9 389.0 10 450.5 11 512.0 12 512.0 13 512.0 14 713.5 15 915.0 16 915.0 17 915.0 18 1582.0 19 2249.0 20 2249.0 21 2249.0 22 3610.0 23 4971.0 24 4971.0 25 4971.0 26 6889.0 27 8807.0 28 8807.0 29 8807.0 30 11199.0 31 13591.0 32 13591.0 33 13591.0 34 14955.5 35 16320.0 36 16320.0 37 16320.0 38 16665.5 39 17011.0 40 17011.0 41 17011.0 42 17514.0 43 18017.0 44 18017.0 45 18017.0 46 18143.0 47 18269.0 48 18269.0 49 18269.0 50 17323.0 51 16377.0 52 16377.0 53 16377.0 54 14333.0 55 12289.0 56 12289.0 57 12289.0 58 10536.0 59 8783.0 60 8783.0 61 8783.0 62 7127.5 63 5472.0 64 5472.0 65 5472.0 66 4220.0 67 2968.0 68 2968.0 69 2968.0 70 2191.5 71 1415.0 72 1415.0 73 1415.0 74 975.5 75 536.0 76 536.0 77 536.0 78 370.0 79 204.0 80 204.0 81 204.0 82 125.5 83 47.0 84 47.0 85 47.0 86 31.0 87 15.0 88 15.0 89 15.0 90 12.0 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4091712626707363 2 0.0 3 0.0 4 0.0 5 0.031780292246270776 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00926925190516231 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 151037.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.57553629201473 #Duplication Level Percentage of deduplicated Percentage of total 1 92.46419101044245 74.50351778473689 2 5.9660895381021515 9.614417281975186 3 0.6966764131215117 1.6840522682778902 4 0.2588527997766654 0.8342881265077731 5 0.12568347531346694 0.5063506713213396 6 0.07876727794971591 0.3808029397836327 7 0.047853944826283995 0.26991000876464216 8 0.028911129433033544 0.18636238070996236 9 0.021932474242008768 0.159049878683459 >10 0.2552138987913692 4.158586290503788 >50 0.029907877500709158 1.7053657533450106 >100 0.02093551425049641 3.0902223519162773 >500 0.004984646250118193 2.9070742634741373 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 811 0.5369545210776168 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 790 0.5230506432198733 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 776 0.513781391314711 No Hit GGTATCAACGCAGAGTACTTTTTTT 669 0.442937823182399 No Hit TATCAACGCAGAGTACTTTTTTTTT 573 0.37937723868985745 No Hit GTCCTACAGTGGACATTTCTAAATT 369 0.24431099664320666 No Hit CTGTAGGACGTGGAATATGGCAAGA 359 0.23769010242523356 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 344 0.22775876109827395 No Hit CTTTAGGACGTGAAATATGGCGAGG 336 0.22246204572389547 No Hit GTCCTAAAGTGTGTATTTCTCATTT 336 0.22246204572389547 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 213 0.14102504684282657 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 179 0.11851400650171812 No Hit GTGGTATCAACGCAGAGTACATGGG 161 0.10659639690936658 No Hit CTGAAGGACCTGGAATATGGCGAGA 156 0.10328594980038004 No Hit GTGGTATCAACGCAGAGTACTTTTT 154 0.10196177095678541 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 6.62089421797308E-4 0.0 11 0.0 0.0 0.0 6.62089421797308E-4 0.0 12 0.0 0.0 0.0 6.62089421797308E-4 0.0 13 0.0 0.0 0.0 6.62089421797308E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTATC 35 0.0021575144 16.286282 2 GGTATCA 195 0.0 14.67422 1 GTGGTAT 40 0.0051117125 14.307366 1 GGCGAGG 90 3.567402E-8 13.718157 19 ACCTTTT 80 1.9721065E-6 13.05863 15 CCTTTTT 75 1.46222155E-5 12.662914 16 CACCTTT 85 3.8900525E-6 12.290475 14 CTTTAGG 125 1.2787496E-9 12.208953 1 TTTAGGA 115 6.8836016E-8 11.56562 2 GACGTGA 85 5.265118E-5 11.17316 7 TTAGGAC 90 9.380985E-5 10.555923 3 TTCCACC 90 9.4125884E-5 10.552428 11 CCACCTT 100 2.3670087E-5 10.446904 13 GTCCTAA 95 1.557077E-4 10.0402565 1 CTGTAGG 220 1.8189894E-12 9.971801 1 TGTAGGA 220 1.6370905E-11 9.500332 2 TAGGACG 230 3.6379788E-12 9.500331 4 ATGGCAA 170 1.6103513E-8 9.497186 17 TCCACCT 120 1.67366E-5 9.497185 12 GTCCTAC 205 3.7289283E-10 9.305605 1 >>END_MODULE