FastQCFastQC Report
Thu 26 May 2016
SRR1512250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320624
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39170.16879080798957521No Hit
GTCCTACAGTGGACATTTCTAAATT35790.1542257599680086No Hit
CTGTAGGACGTGGAATATGGCAAGA30960.13341239252890602No Hit
GTCCTAAAGTGTGTATTTCTCATTT27320.11772695619798813No Hit
CTTTAGGACGTGAAATATGGCGAGG25860.11143554492240018No Hit
GGTATCAACGCAGAGTACTTTTTTT25410.10949641131006144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6750.011.8675854
GTTGCAC1157.910057E-811.473013
GGTATCA12550.011.20981
TAGGACA5900.011.1813244
GTCCTAA8650.010.9604751
TACACCG951.48708605E-510.9122995
GACGCTC1053.8179023E-610.77034958
GTCCTAG3050.010.6957851
GTAGGAC19800.010.5189963
TGTAGGA19800.010.4518552
CTGTAGG19100.010.4079161
TAGACAG3250.010.1492015
CCTATAC2055.456968E-1210.1138383
GGCGAGG8950.010.10957319
GTCCTAC18600.010.0848031
GGACGTG19350.010.0817656
AGGACGT19550.010.0750395
GTATCAA24300.010.06856251
TCCTAGA2950.010.0216322
TCCAACG2450.010.00211618
Produced by FastQC (version 0.11.5)