FastQCFastQC Report
Thu 26 May 2016
SRR1512250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320624
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52830.2276542860885693No Hit
GTCCTACAGTGGACATTTCTAAATT35900.1546997704065803No Hit
TATCAACGCAGAGTACTTTTTTTTT35160.15151097291073434No Hit
CTGTAGGACGTGGAATATGGCAAGA33690.14517646977709445No Hit
GGTATCAACGCAGAGTACTTTTTTT33420.14401298960969117No Hit
GTCCTAAAGTGTGTATTTCTCATTT27920.12031246768110646No Hit
CTTTAGGACGTGAAATATGGCGAGG27830.11992464095863871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG500.001499153813.3009215
TAGGACC6550.012.3290974
AGGACCG1501.4551915E-1112.0341655
TGCGTCC752.0803898E-411.39661210
GGTATCA15050.011.3428141
GATCGTC700.001495585510.8539167
TTAGGAC12350.010.8469043
TGTAGGA19800.010.7962012
GGCGAGG9550.010.64104619
AGGACGT19200.010.5892745
GTAGGAC18550.010.4481623
CTAGGAC3100.010.4200763
GACGTGA11050.010.3996257
AGGACTG4700.010.3092235
GGACGTG19450.010.2540226
TAGGACG20300.010.1558744
GTCCTAC16950.010.1276131
TAGGACT3700.010.0142074
TCCAACG1901.0004442E-109.99702918
CGGTTTC1054.1200088E-59.94942413
Produced by FastQC (version 0.11.5)