##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512250_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2320624 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.299720678576108 31.0 30.0 34.0 26.0 34.0 2 30.500324912609713 31.0 30.0 34.0 27.0 34.0 3 30.576207089127752 31.0 30.0 34.0 27.0 34.0 4 34.188869890167474 37.0 35.0 37.0 30.0 37.0 5 33.71330728286875 37.0 35.0 37.0 28.0 37.0 6 33.62267519425809 37.0 35.0 37.0 28.0 37.0 7 33.41694432187205 37.0 35.0 37.0 28.0 37.0 8 33.299593988513436 36.0 35.0 37.0 27.0 37.0 9 34.709975851322746 38.0 35.0 39.0 27.0 39.0 10 34.489913488785774 38.0 35.0 39.0 27.0 39.0 11 34.47112457683795 38.0 35.0 39.0 27.0 39.0 12 34.24673191348534 38.0 34.0 39.0 27.0 39.0 13 34.2225591047925 38.0 34.0 39.0 27.0 39.0 14 35.12244206730603 39.0 34.0 40.0 26.0 41.0 15 35.03996295823882 39.0 34.0 40.0 26.0 41.0 16 34.92165598563145 38.0 34.0 40.0 25.0 41.0 17 34.823589689669674 38.0 34.0 40.0 25.0 41.0 18 34.73672382945277 38.0 34.0 40.0 25.0 41.0 19 34.75307632774633 39.0 34.0 40.0 25.0 41.0 20 34.598422665627865 39.0 34.0 40.0 24.0 41.0 21 34.47237208612856 38.0 34.0 40.0 24.0 41.0 22 34.561945838705455 39.0 34.0 40.0 24.0 41.0 23 34.430909962148114 39.0 34.0 40.0 23.0 41.0 24 34.266162032280974 39.0 34.0 40.0 19.0 41.0 25 33.77001530622798 38.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2448656452482858 1101 2 0.3237784137231685 1101 3 0.4192509390066732 1101 4 0.45072727349434416 1101 5 0.3102845484495731 1101 6 0.39149973347176115 1101 7 0.32477919617470974 1101 8 0.11743382890998788 1101 9 0.2879336181315466 1101 10 0.3234012261292136 1101 11 0.2609463587539622 1101 12 0.3101630496213872 1101 13 0.27639532313792614 1101 14 0.22235632433883268 1101 15 0.27139815593864824 1101 16 0.18973235968756796 1101 17 0.14887837420508276 1101 18 0.2800295039920542 1101 19 0.17806026863301128 1101 20 0.1796563829371891 1101 21 0.2149286997959976 1101 22 0.28265824518599914 1101 23 0.13147548417816068 1101 24 0.03909521082134404 1101 25 -0.04061641835063057 1102 1 0.5324777410522117 1102 2 0.43770103299227614 1102 3 0.39752884977655256 1102 4 0.35677042081789523 1102 5 0.5451424674057037 1102 6 0.46124451267041877 1102 7 0.4394960470319944 1102 8 0.6983253445651556 1102 9 0.8481511325711821 1102 10 0.8296577120782231 1102 11 0.869255195355592 1102 12 0.8103720470538818 1102 13 0.8143875834110545 1102 14 0.8954338993897863 1102 15 1.1038733801302527 1102 16 0.932439362238533 1102 17 1.236155577980682 1102 18 1.1405715742863052 1102 19 1.0516434893020445 1102 20 1.0971123198286463 1102 21 1.0969224650160143 1102 22 1.071212793309158 1102 23 1.1410943274362921 1102 24 1.2291954303616706 1102 25 1.1171233085263097 1103 1 0.4305758611696895 1103 2 0.3121399021297826 1103 3 0.334427340876541 1103 4 0.41586504117516654 1103 5 0.3850771887526534 1103 6 0.4725931688496985 1103 7 0.6213799326774279 1103 8 0.4547707693123826 1103 9 0.7573486054510141 1103 10 0.6786597057128603 1103 11 0.5568794011716136 1103 12 0.7970731564342728 1103 13 0.8352809140492141 1103 14 0.8448153970334928 1103 15 0.8807779353674121 1103 16 1.0682221313693034 1103 17 0.85078738973084 1103 18 1.0213611407995842 1103 19 0.9163383205168998 1103 20 0.8113208310235152 1103 21 0.9902386364448077 1103 22 1.0404787156327089 1103 23 1.0805302437749802 1103 24 0.9729541252642306 1103 25 1.2123760403606525 1104 1 0.36182010310792023 1104 2 0.4254304473081234 1104 3 0.3876701946224941 1104 4 0.3249875438490264 1104 5 0.5029489609890021 1104 6 0.41556272585077636 1104 7 0.2963346142644312 1104 8 0.28521299808300427 1104 9 0.6352398624389082 1104 10 0.5703273751767668 1104 11 0.7210865672088431 1104 12 0.570366724444618 1104 13 0.6160050652254725 1104 14 0.9178637690844269 1104 15 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-0.018862972434703806 2313 13 -0.2910520876387608 2313 14 -0.15786559661969335 2313 15 -0.11885759172908195 2313 16 -0.16727651845991431 2313 17 -0.3452017698584413 2313 18 -0.32846870212322443 2313 19 -0.07891289909903065 2313 20 -0.2330885625116963 2313 21 -0.2796112795050547 2313 22 0.05192623258044193 2313 23 -0.0059267031029648365 2313 24 -0.14990252654927616 2313 25 0.11043936626392536 2314 1 -0.46985353759485804 2314 2 -0.5067776562232531 2314 3 -0.49128101271751845 2314 4 -0.47001162438520083 2314 5 -0.7433697290012304 2314 6 -0.6003065120385003 2314 7 -0.6752002281905973 2314 8 -0.571231053938611 2314 9 -0.730545985599619 2314 10 -0.7017658167137313 2314 11 -0.7273861828647341 2314 12 -0.7490953150450608 2314 13 -0.9266824339747544 2314 14 -0.8051087485212847 2314 15 -0.8147267989962401 2314 16 -0.8283108616695856 2314 17 -0.8262884449896575 2314 18 -0.6148812469559886 2314 19 -0.6501056282365454 2314 20 -0.8172309708304368 2314 21 -0.8167253933686922 2314 22 -0.6915236273219989 2314 23 -0.7657757004863726 2314 24 -0.7273795656870092 2314 25 -0.8166604752574713 2315 1 -0.515521089725322 2315 2 -0.46930680679426473 2315 3 -0.48287543137648115 2315 4 -0.4745542482771512 2315 5 -0.4727241512928657 2315 6 -0.697269825294832 2315 7 -0.8607138134108112 2315 8 -0.6313556675617846 2315 9 -0.7670697455448732 2315 10 -0.7213908406342284 2315 11 -0.6679905362328995 2315 12 -0.529541160662987 2315 13 -0.6008302966986747 2315 14 -0.942173841462548 2315 15 -0.8115086408258065 2315 16 -1.131387442936024 2315 17 -1.0322124572758753 2315 18 -0.9251492213845722 2315 19 -1.256035154399889 2315 20 -1.094660401311799 2315 21 -1.087272045634407 2315 22 -0.9140160295236583 2315 23 -1.05643681435442 2315 24 -0.855744084860703 2315 25 -0.7825506582857571 2316 1 -0.8577680756851116 2316 2 -1.0125612996683167 2316 3 -0.8792477688017257 2316 4 -1.0426826671970204 2316 5 -1.1094919576159228 2316 6 -1.214195882287953 2316 7 -1.2113596486478855 2316 8 -1.1343044602693126 2316 9 -1.3957123476613233 2316 10 -1.4685534781716925 2316 11 -1.4114779479022417 2316 12 -1.7592366964189452 2316 13 -1.7492604779256666 2316 14 -2.056336757082569 2316 15 -1.782880600242848 2316 16 -1.906730427859742 2316 17 -1.9124017703593594 2316 18 -1.9998175109664729 2316 19 -1.8054912827675338 2316 20 -2.0153039882218025 2316 21 -1.8634341579048126 2316 22 -1.9475210333661934 2316 23 -1.905340689168618 2316 24 -1.9322945266043803 2316 25 -1.827328195526139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51014.0 3 1.0 4 959.0 5 8162.0 6 9849.0 7 7978.0 8 6505.0 9 5671.0 10 5414.0 11 5032.0 12 4947.0 13 4711.0 14 4589.0 15 4598.0 16 4767.0 17 4986.0 18 5384.0 19 6132.0 20 7324.0 21 9580.0 22 12090.0 23 14993.0 24 18349.0 25 21612.0 26 25021.0 27 28304.0 28 33274.0 29 40442.0 30 50702.0 31 64866.0 32 82147.0 33 105573.0 34 134851.0 35 176196.0 36 250338.0 37 414187.0 38 690076.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.60578762610246 19.633998928794234 14.268149280051084 24.492064165052216 2 15.486481222291934 20.450620178021083 39.85634898199795 24.206549617689035 3 19.191907004322974 24.171041926654212 29.854814911851296 26.782236157171518 4 13.431904522231953 17.15521342535456 36.852070822330546 32.56081123008294 5 13.833286564015557 37.44442102386442 35.1529830850249 13.569309327095125 6 32.92562690035094 36.678410634381095 17.157324926399106 13.238637538868856 7 29.016893732030695 31.27464854280573 21.25109453319452 18.457363191969055 8 25.673137914629855 37.144664538503434 19.651869497169727 17.530328049696976 9 26.206442749881063 15.700604664952186 19.874611311440372 38.218341273726374 10 15.486136487427519 29.21989947531354 33.3190986562235 21.974865381035446 11 35.13055109315425 22.333044905163437 23.632178241714296 18.904225759968007 12 23.641486083053522 24.376288446555755 30.921036755631242 21.061188714759478 13 29.176161239390787 21.471509387130357 25.653229476209848 23.69909989726901 14 21.324178324450667 21.80753969621964 26.918535704189907 29.949746275139788 15 23.390993112197407 30.53747612711064 22.94572494294638 23.125805817745572 16 22.80369417880708 28.05878936010314 26.867256393108065 22.270260067981713 17 22.238027358158842 28.469799502202857 27.223022773185146 22.06915036645316 18 22.072985541819786 27.43180282544695 29.025124276918625 21.470087355814645 19 23.861383834692738 26.810547507911664 27.138174904680813 22.189893752714788 20 23.828935665579603 27.043545184398678 27.67785733492371 21.449661815098008 21 23.450546060025236 26.530665889864107 26.932066547618227 23.086721502492434 22 23.603694523541943 27.28563524293466 27.124083867097816 21.98658636642558 23 22.982827032729126 27.17570791304408 27.361563096822234 22.47990195740456 24 23.49260371348396 26.500803232234087 27.85483559594316 22.151757458338793 25 23.191200077570326 26.951335394692897 27.641021773559295 22.216442754177486 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 174.0 1 174.0 2 303.0 3 432.0 4 432.0 5 432.0 6 1163.5 7 1895.0 8 1895.0 9 1895.0 10 2814.5 11 3734.0 12 3734.0 13 3734.0 14 6207.5 15 8681.0 16 8681.0 17 8681.0 18 15849.0 19 23017.0 20 23017.0 21 23017.0 22 37186.5 23 51356.0 24 51356.0 25 51356.0 26 75224.5 27 99093.0 28 99093.0 29 99093.0 30 129424.0 31 159755.0 32 159755.0 33 159755.0 34 183702.0 35 207649.0 36 207649.0 37 207649.0 38 228432.0 39 249215.0 40 249215.0 41 249215.0 42 268048.0 43 286881.0 44 286881.0 45 286881.0 46 295466.5 47 304052.0 48 304052.0 49 304052.0 50 298499.0 51 292946.0 52 292946.0 53 292946.0 54 265646.5 55 238347.0 56 238347.0 57 238347.0 58 205889.0 59 173431.0 60 173431.0 61 173431.0 62 142550.5 63 111670.0 64 111670.0 65 111670.0 66 86807.5 67 61945.0 68 61945.0 69 61945.0 70 45445.5 71 28946.0 72 28946.0 73 28946.0 74 20234.5 75 11523.0 76 11523.0 77 11523.0 78 7906.0 79 4289.0 80 4289.0 81 4289.0 82 2732.0 83 1175.0 84 1175.0 85 1175.0 86 756.0 87 337.0 88 337.0 89 337.0 90 203.0 91 69.0 92 69.0 93 69.0 94 40.0 95 11.0 96 11.0 97 11.0 98 6.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39687601265866423 2 0.0 3 0.0 4 0.0 5 0.03132778080378381 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006420686849743861 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2320624.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.71731285667732 #Duplication Level Percentage of deduplicated Percentage of total 1 85.35067505932764 62.91822415877063 2 9.855338584228734 14.53018155444146 3 2.394127931025129 5.2946603313086715 4 0.9230782168529408 2.721873828117283 5 0.4598916470510281 1.6950988212916633 6 0.2678896223048799 1.1848861859103572 7 0.16447524878582698 0.8487271360347252 8 0.11255151611750712 0.6637596260902107 9 0.08354579811227941 0.5542894563573333 >10 0.3571923325292983 4.353653437196392 >50 0.017126253665208754 0.8751143719601713 >100 0.011067229066473392 1.623576478083529 >500 0.0019880590715330017 1.03088069823335 >1k 9.940295357640304E-4 1.4773542837926095 >5k 5.847232563317826E-5 0.22771963241163434 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5283 0.2276542860885693 No Hit GTCCTACAGTGGACATTTCTAAATT 3590 0.1546997704065803 No Hit TATCAACGCAGAGTACTTTTTTTTT 3516 0.15151097291073434 No Hit CTGTAGGACGTGGAATATGGCAAGA 3369 0.14517646977709445 No Hit GGTATCAACGCAGAGTACTTTTTTT 3342 0.14401298960969117 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2792 0.12031246768110646 No Hit CTTTAGGACGTGAAATATGGCGAGG 2783 0.11992464095863871 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.618371610394445E-5 0.0 0.0 0.0 6 0.0 8.618371610394445E-5 0.0 0.0 4.3091858051972226E-5 7 0.0 1.292755741559167E-4 0.0 0.0 4.3091858051972226E-5 8 0.0 1.292755741559167E-4 0.0 0.0 4.3091858051972226E-5 9 0.0 1.292755741559167E-4 0.0 1.292755741559167E-4 4.3091858051972226E-5 10 4.3091858051972226E-5 1.292755741559167E-4 0.0 1.292755741559167E-4 4.3091858051972226E-5 11 4.3091858051972226E-5 1.292755741559167E-4 0.0 1.292755741559167E-4 4.3091858051972226E-5 12 4.3091858051972226E-5 1.292755741559167E-4 0.0 1.292755741559167E-4 4.3091858051972226E-5 13 4.3091858051972226E-5 1.292755741559167E-4 0.0 1.292755741559167E-4 4.3091858051972226E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCCG 50 0.0014991538 13.300921 5 TAGGACC 655 0.0 12.329097 4 AGGACCG 150 1.4551915E-11 12.034165 5 TGCGTCC 75 2.0803898E-4 11.396612 10 GGTATCA 1505 0.0 11.342814 1 GATCGTC 70 0.0014955855 10.853916 7 TTAGGAC 1235 0.0 10.846904 3 TGTAGGA 1980 0.0 10.796201 2 GGCGAGG 955 0.0 10.641046 19 AGGACGT 1920 0.0 10.589274 5 GTAGGAC 1855 0.0 10.448162 3 CTAGGAC 310 0.0 10.420076 3 GACGTGA 1105 0.0 10.399625 7 AGGACTG 470 0.0 10.309223 5 GGACGTG 1945 0.0 10.254022 6 TAGGACG 2030 0.0 10.155874 4 GTCCTAC 1695 0.0 10.127613 1 TAGGACT 370 0.0 10.014207 4 TCCAACG 190 1.0004442E-10 9.997029 18 CGGTTTC 105 4.1200088E-5 9.949424 13 >>END_MODULE