FastQCFastQC Report
Thu 26 May 2016
SRR1512249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441761
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT74190.303838090623939No Hit
CTGTAGGACGTGGAATATGGCAAGA65570.26853570025895246No Hit
GTCCTAAAGTGTGTATTTCTCATTT56300.2305712966993903No Hit
CTTTAGGACGTGAAATATGGCGAGG52280.2141077689421692No Hit
GTATCAACGCAGAGTACTTTTTTTT48130.19711183854603295No Hit
GGTATCAACGCAGAGTACTTTTTTT30010.12290310149109598No Hit
GTCCTACAGTGTGCATTTCTCATTT28220.11557232669372637No Hit
TATCAACGCAGAGTACTTTTTTTTT27780.11377034853124446No Hit
CTGAAGGACCTGGAATATGGCGAGA26770.10963398956736553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTC653.6558722E-614.5027887
CCAACGA2450.014.23757619
GCGTAAA508.9696946E-414.23721
TATGCCG400.00555815814.1402195
TCCAACG3750.014.07852618
TAGGACC10750.013.3290734
TTACGGT604.369386E-412.5690824
CGGTTTC3300.011.71233913
CGATATT708.4410707E-411.6222041
TAAGACG658.564106E-411.602234
GTATAGG1809.094947E-1211.2993651
GGTATCA13800.011.2011091
CGTTAGG1206.347236E-711.0168811
ACGTAGT650.006423403510.9516921
GGCGAGG16000.010.782819
ACACCGT700.00159132110.77349956
GTCCTAT2650.010.7450561
TGTAGGA33750.010.69673252
GCCGGTT3000.010.683511
ATCCCGT2650.010.67186310
Produced by FastQC (version 0.11.5)