##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512249_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2441761 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.54258258691166 31.0 30.0 34.0 25.0 34.0 2 26.675630006376544 31.0 27.0 34.0 2.0 34.0 3 28.323296997535795 31.0 28.0 34.0 16.0 34.0 4 32.887892385864134 35.0 32.0 37.0 27.0 37.0 5 33.29317652300942 35.0 35.0 37.0 28.0 37.0 6 33.50241116964355 36.0 35.0 37.0 28.0 37.0 7 33.50630712833893 37.0 35.0 37.0 28.0 37.0 8 33.51110366657507 37.0 35.0 37.0 28.0 37.0 9 35.036116556862034 39.0 35.0 39.0 28.0 39.0 10 34.89241084610656 39.0 35.0 39.0 28.0 39.0 11 34.9129173575956 39.0 35.0 39.0 28.0 39.0 12 34.799830532144625 39.0 35.0 39.0 27.0 39.0 13 34.802683391208234 39.0 35.0 39.0 27.0 39.0 14 35.871663115268035 40.0 36.0 41.0 27.0 41.0 15 35.81072717600126 40.0 36.0 41.0 27.0 41.0 16 35.75753769513069 40.0 36.0 41.0 27.0 41.0 17 35.74273526360688 40.0 36.0 41.0 27.0 41.0 18 35.69270825441147 40.0 36.0 41.0 27.0 41.0 19 35.71366976538654 40.0 36.0 41.0 27.0 41.0 20 35.638911015451555 40.0 36.0 41.0 27.0 41.0 21 35.5578203599779 40.0 36.0 41.0 27.0 41.0 22 35.45693169806545 39.0 36.0 41.0 27.0 41.0 23 35.34278334366058 39.0 36.0 41.0 27.0 41.0 24 35.237045312788595 39.0 36.0 41.0 26.0 41.0 25 35.105066794006454 39.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5356495830985146 1101 2 3.5412644971128557 1101 3 2.0539033124725776 1101 4 0.9624504853556175 1101 5 0.7179781459122694 1101 6 0.6989517225083688 1101 7 0.4372241471255478 1101 8 0.3419164838155595 1101 9 0.3649255602569639 1101 10 0.3077985333471247 1101 11 0.4201405998519476 1101 12 0.38411337965155923 1101 13 0.311488975648615 1101 14 0.39763364534584156 1101 15 0.5359230530078136 1101 16 0.6262434350025714 1101 17 0.49733234748675414 1101 18 0.4542493656394271 1101 19 0.2585377158902702 1101 20 0.48644202872193176 1101 21 0.4935339784176449 1101 22 0.3985604624652481 1101 23 0.2651143134610834 1101 24 0.1959549156154594 1101 25 0.3428240499655786 1102 1 1.1439757937784378 1102 2 4.051974633118235 1102 3 2.4940892615619745 1102 4 1.5999166428776448 1102 5 1.050845939459066 1102 6 1.0367025930695206 1102 7 0.9162598478129524 1102 8 0.8866679001396065 1102 9 0.9136965580410887 1102 10 1.010465714705596 1102 11 0.8996809863939106 1102 12 0.9804206941909186 1102 13 0.9739872004709795 1102 14 1.0732066568879617 1102 15 1.0105664946458504 1102 16 0.9355323054243456 1102 17 1.0420010831021074 1102 18 1.1220767220001306 1102 19 1.022987925163747 1102 20 1.037645349010674 1102 21 1.2035688710810533 1102 22 1.1303816340982848 1102 23 1.053024076683542 1102 24 1.1323028823809764 1102 25 1.1330732734954196 1103 1 0.8017596210394942 1103 2 3.654370133228131 1103 3 2.2935568866192213 1103 4 1.2566282009292067 1103 5 0.8958022423197392 1103 6 0.6619757214553417 1103 7 0.4413543156999964 1103 8 0.6313536774573763 1103 9 0.5678709396702573 1103 10 0.6507689850331175 1103 11 0.6473219260848495 1103 12 0.5514897038925497 1103 13 0.6368906326170745 1103 14 0.8175942130532334 1103 15 0.9110283376491708 1103 16 0.8194436400056802 1103 17 0.8782875373631853 1103 18 0.8355772088848639 1103 19 0.8305774408444293 1103 20 0.9236255184098141 1103 21 0.9616909318249469 1103 22 1.0714359181735773 1103 23 1.0576433573190016 1103 24 1.0019280117575775 1103 25 1.1117787861465942 1104 1 1.301573807510131 1104 2 4.055063903850428 1104 3 2.572839629701047 1104 4 1.596662285107243 1104 5 1.3146990608849336 1104 6 1.1022828329882017 1104 7 1.0394141085905346 1104 8 0.9843264268097869 1104 9 1.1488826334014774 1104 10 1.0223477956796145 1104 11 0.930228283411779 1104 12 0.9557843837824151 1104 13 1.162901354708822 1104 14 1.2282334316280412 1104 15 0.8410015123860148 1104 16 1.113497974352505 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2315 21 -1.3867304903804722 2315 22 -1.486496806594829 2315 23 -1.3275323252909814 2315 24 -1.3344684016704562 2315 25 -1.1323457716085557 2316 1 -1.3896777646757812 2316 2 -0.0944356783058069 2316 3 -0.33671832167247473 2316 4 -1.3117747850432444 2316 5 -1.3798685245287459 2316 6 -1.4321090272783437 2316 7 -1.4002974256321536 2316 8 -1.347432134451715 2316 9 -1.566461598723265 2316 10 -1.6499920291522585 2316 11 -2.126663878838599 2316 12 -1.6513029021969885 2316 13 -1.5597656073370274 2316 14 -1.8322846055200301 2316 15 -1.9620348455123349 2316 16 -1.7454802715121502 2316 17 -1.90065176455159 2316 18 -2.314976564686006 2316 19 -2.285260134000545 2316 20 -2.1689202876520355 2316 21 -2.104257378329649 2316 22 -2.0970088522673365 2316 23 -1.8245255735354604 2316 24 -1.7608668646645285 2316 25 -1.7863041411464877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83972.0 3 1.0 4 741.0 5 3815.0 6 4446.0 7 4102.0 8 3781.0 9 3658.0 10 3493.0 11 3343.0 12 3334.0 13 3316.0 14 3373.0 15 3509.0 16 3713.0 17 4038.0 18 4553.0 19 5424.0 20 7334.0 21 9368.0 22 11642.0 23 13856.0 24 16496.0 25 19197.0 26 21535.0 27 24516.0 28 29105.0 29 35594.0 30 44742.0 31 58072.0 32 76933.0 33 103369.0 34 142792.0 35 202620.0 36 291139.0 37 374434.0 38 816405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.86400716532044 19.375770192086776 13.563571537099659 25.196651105493125 2 15.178324089779045 20.576050232859806 41.78323647961742 22.462389197743725 3 18.675824538109996 24.22374671394948 29.92770381704024 27.172724930900284 4 11.98643110443651 16.893627181366234 38.73954084777339 32.38040086642386 5 12.715495087357034 37.99479146402944 36.28057782887023 13.009135619743292 6 31.21903612849272 37.493780093596534 18.382500797581415 12.90468298032933 7 27.710082958066344 31.61789731712262 22.307917570276807 18.36410215453423 8 24.06550026804425 38.707719551585924 20.66500365924429 16.56177652112553 9 25.832053178013737 15.793069018630407 20.903970536018882 37.47090726733697 10 14.91324172113419 29.413159605171707 34.71764600361474 20.95595267007936 11 33.6250280541326 22.37138920788235 25.519800074374167 18.48378266361088 12 23.369512244067174 24.48420274933511 32.39662963590927 19.749655370688448 13 28.762397302602505 22.31729477209276 26.1416248355183 22.778683089786426 14 20.058998620662543 23.126591070673843 27.83292488344435 28.981485425219265 15 22.11272929660192 32.62522417222652 23.411709827456495 21.850336703715065 16 20.98292994277491 28.501929550025572 29.442070702251367 21.07306980494815 17 20.774973789397812 29.399121944826685 28.81843424415176 21.007470021623746 18 20.512097960012877 27.824611732362996 31.510072144587355 20.153218163036772 19 22.998569016881014 27.0409529839725 27.963924809086212 21.99655319006028 20 22.922145123949477 28.003928312394212 28.97875754424778 20.095169019408534 21 22.019138458110664 27.271360793192596 27.631865647434278 23.077635101262466 22 22.347093777350207 29.124355113570044 27.964552696492497 20.56399841258725 23 21.364570509307836 28.62469928812068 28.579607864337476 21.431122338234005 24 22.66238572166403 27.013164356422664 29.496251715634177 20.82819820627913 25 22.032224638660555 27.888729158219373 29.023226477611086 21.055819725508986 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 230.0 1 230.0 2 385.0 3 540.0 4 540.0 5 540.0 6 1258.0 7 1976.0 8 1976.0 9 1976.0 10 3096.5 11 4217.0 12 4217.0 13 4217.0 14 7516.0 15 10815.0 16 10815.0 17 10815.0 18 19825.0 19 28835.0 20 28835.0 21 28835.0 22 47942.5 23 67050.0 24 67050.0 25 67050.0 26 98758.0 27 130466.0 28 130466.0 29 130466.0 30 172550.5 31 214635.0 32 214635.0 33 214635.0 34 237603.0 35 260571.0 36 260571.0 37 260571.0 38 274779.5 39 288988.0 40 288988.0 41 288988.0 42 301854.5 43 314721.0 44 314721.0 45 314721.0 46 316515.5 47 318310.0 48 318310.0 49 318310.0 50 300292.5 51 282275.0 52 282275.0 53 282275.0 54 244852.5 55 207430.0 56 207430.0 57 207430.0 58 175245.0 59 143060.0 60 143060.0 61 143060.0 62 115198.0 63 87336.0 64 87336.0 65 87336.0 66 67134.5 67 46933.0 68 46933.0 69 46933.0 70 34020.5 71 21108.0 72 21108.0 73 21108.0 74 14639.5 75 8171.0 76 8171.0 77 8171.0 78 5483.0 79 2795.0 80 2795.0 81 2795.0 82 1854.5 83 914.0 84 914.0 85 914.0 86 586.5 87 259.0 88 259.0 89 259.0 90 175.0 91 91.0 92 91.0 93 91.0 94 60.5 95 30.0 96 30.0 97 30.0 98 17.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.305465194996562 3 0.0 4 0.0 5 0.0 6 1.638161965892649E-4 7 0.006798372158454493 8 0.0 9 0.0 10 0.0037677725215530923 11 0.0021705646048077597 12 2.866783440312135E-4 13 0.0 14 3.68586442325846E-4 15 0.0 16 0.0 17 4.095404914731622E-5 18 0.01126236351551196 19 0.0033991860792272465 20 0.0 21 0.0075764990922535 22 0.002129610555660443 23 0.002252472703102392 24 0.009992787991945157 25 0.006184061421244749 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2441761.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.09736615598167 #Duplication Level Percentage of deduplicated Percentage of total 1 87.30847172322294 64.69327797794821 2 8.952645744190928 13.267349395442125 3 1.9120508496688282 4.250337957503011 4 0.6785450586227367 2.0111360664840388 5 0.32566141087839895 1.206532640236516 6 0.192091782456778 0.8540097084153032 7 0.1321057150865698 0.6852079879447146 8 0.08253157420865846 0.48922978188548316 9 0.06446337563078265 0.42989097129882187 >10 0.31037700812148195 4.047188915517631 >50 0.02010137818816735 1.0350683360398731 >100 0.01687049730055454 2.5828508465401345 >500 0.00195802581932649 1.0295614626548186 >1k 0.001902082224478775 2.388920847629495 >5k 2.2377437935044414E-4 1.0294371044600188 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7419 0.303838090623939 No Hit CTGTAGGACGTGGAATATGGCAAGA 6557 0.26853570025895246 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5630 0.2305712966993903 No Hit CTTTAGGACGTGAAATATGGCGAGG 5228 0.2141077689421692 No Hit GTATCAACGCAGAGTACTTTTTTTT 4813 0.19711183854603295 No Hit GGTATCAACGCAGAGTACTTTTTTT 3001 0.12290310149109598 No Hit GTCCTACAGTGTGCATTTCTCATTT 2822 0.11557232669372637 No Hit TATCAACGCAGAGTACTTTTTTTTT 2778 0.11377034853124446 No Hit CTGAAGGACCTGGAATATGGCGAGA 2677 0.10963398956736553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.095404914731622E-5 0.0 5 0.0 0.0 0.0 4.095404914731622E-5 0.0 6 4.095404914731622E-5 0.0 0.0 4.095404914731622E-5 0.0 7 4.095404914731622E-5 0.0 0.0 4.095404914731622E-5 0.0 8 4.095404914731622E-5 0.0 0.0 4.095404914731622E-5 0.0 9 4.095404914731622E-5 0.0 0.0 4.095404914731622E-5 4.095404914731622E-5 10 4.095404914731622E-5 0.0 0.0 8.190809829463245E-5 4.095404914731622E-5 11 4.095404914731622E-5 0.0 0.0 1.2286214744194866E-4 4.095404914731622E-5 12 4.095404914731622E-5 0.0 0.0 1.2286214744194866E-4 4.095404914731622E-5 13 4.095404914731622E-5 0.0 0.0 1.2286214744194866E-4 4.095404914731622E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGTC 65 3.6558722E-6 14.502788 7 CCAACGA 245 0.0 14.237576 19 GCGTAAA 50 8.9696946E-4 14.2372 1 TATGCCG 40 0.005558158 14.140219 5 TCCAACG 375 0.0 14.078526 18 TAGGACC 1075 0.0 13.329073 4 TTACGGT 60 4.369386E-4 12.569082 4 CGGTTTC 330 0.0 11.712339 13 CGATATT 70 8.4410707E-4 11.622204 1 TAAGACG 65 8.564106E-4 11.60223 4 GTATAGG 180 9.094947E-12 11.299365 1 GGTATCA 1380 0.0 11.201109 1 CGTTAGG 120 6.347236E-7 11.016881 1 ACGTAGT 65 0.0064234035 10.951692 1 GGCGAGG 1600 0.0 10.7828 19 ACACCGT 70 0.001591321 10.7734995 6 GTCCTAT 265 0.0 10.745056 1 TGTAGGA 3375 0.0 10.6967325 2 GCCGGTT 300 0.0 10.6835 11 ATCCCGT 265 0.0 10.671863 10 >>END_MODULE