FastQCFastQC Report
Thu 26 May 2016
SRR1512249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441761
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69200.2834020200994282No Hit
CTGTAGGACGTGGAATATGGCAAGA66570.2726311051736841No Hit
GTATCAACGCAGAGTACTTTTTTTT59900.24531475439242417No Hit
CTTTAGGACGTGAAATATGGCGAGG56430.23110369933830543No Hit
GTCCTAAAGTGTGTATTTCTCATTT54260.22221667067333783No Hit
GGTATCAACGCAGAGTACTTTTTTT41650.17057361469857205No Hit
TATCAACGCAGAGTACTTTTTTTTT41480.1698773958630677No Hit
CTGAAGGACCTGGAATATGGCGAGA32000.13105295727141192No Hit
GTCCTACAGTGTGCATTTCTCATTT26920.11024830030457528No Hit
TTGTAGAACAGTGTATATCAATGAG25620.10492427391542417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9900.012.6667084
TCCAACG3200.012.16867418
GGCGAGG14900.011.92041619
GGTATCA17950.011.8449671
CCAACGA2050.011.58303119
AGCGTGC752.0798143E-411.3970018
AATGTCC6900.011.1492418
TATGTCG2100.010.85428816
TGTAGGA34850.010.7948692
CACCTTT10750.010.69021114
AAATGTC6950.010.6590667
ACCTTTT10700.010.4738815
CTGTAGG35100.010.4579621
ACTGTTC10050.010.3952758
CTAGGAC3300.010.3636693
GTAGGAC35000.010.2600333
GGACGTG35450.010.2074716
AGGACGT35900.010.16159255
ATGTCGA2450.010.07898217
CCACCTT10750.010.0717713
Produced by FastQC (version 0.11.5)