##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512249_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2441761 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.870508620622577 31.0 30.0 34.0 26.0 34.0 2 30.084054909550936 31.0 30.0 34.0 26.0 34.0 3 30.152693895921836 31.0 30.0 34.0 26.0 34.0 4 33.75515294084884 35.0 35.0 37.0 30.0 37.0 5 33.232621865940196 35.0 35.0 37.0 27.0 37.0 6 33.14622192753509 35.0 33.0 37.0 27.0 37.0 7 32.91818855326135 35.0 33.0 37.0 26.0 37.0 8 32.78943557539006 35.0 33.0 37.0 26.0 37.0 9 34.14413286148808 38.0 34.0 39.0 26.0 39.0 10 33.88595484979898 37.0 33.0 39.0 25.0 39.0 11 33.86377126999735 37.0 34.0 39.0 25.0 39.0 12 33.63839048948689 37.0 33.0 39.0 24.0 39.0 13 33.621848739495796 37.0 33.0 39.0 24.0 39.0 14 34.42871476774344 38.0 33.0 40.0 24.0 41.0 15 34.34193477576225 38.0 33.0 40.0 22.0 41.0 16 34.214754842918694 38.0 33.0 40.0 22.0 41.0 17 34.113999281665976 38.0 33.0 40.0 19.0 41.0 18 34.03545965391371 38.0 33.0 40.0 19.0 41.0 19 34.04956259027808 38.0 33.0 40.0 18.0 41.0 20 33.890903737097936 38.0 33.0 40.0 18.0 41.0 21 33.74339790012208 38.0 33.0 40.0 18.0 41.0 22 33.827351243631135 38.0 33.0 40.0 18.0 41.0 23 33.6831536747454 38.0 33.0 40.0 16.0 41.0 24 33.51042055303529 38.0 33.0 40.0 10.0 41.0 25 33.013136011264 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4517574311128598 1101 2 0.33489534939085175 1101 3 0.43009368148655724 1101 4 0.5138107598017569 1101 5 0.5635635832059975 1101 6 0.662668900171191 1101 7 0.5138450161993617 1101 8 0.3500947023996659 1101 9 0.41800622652304753 1101 10 0.5503785904737626 1101 11 0.5937797859161122 1101 12 0.5224576896975037 1101 13 0.39829636431483095 1101 14 0.3499995487915726 1101 15 0.43559806931209266 1101 16 0.35281056326234506 1101 17 0.5213992702594439 1101 18 0.5042071638430698 1101 19 0.3826301925994926 1101 20 0.42422957587010046 1101 21 0.4337084691115294 1101 22 0.267331669143573 1101 23 0.22680454410777884 1101 24 0.08391643970311691 1101 25 0.22212700107998273 1102 1 0.27750020142216414 1102 2 0.37954661848706905 1102 3 0.30865766327714184 1102 4 0.3673019483378255 1102 5 0.3467415873990163 1102 6 0.5823357267574423 1102 7 0.3884571760915705 1102 8 0.4063537461507494 1102 9 0.49093640450774956 1102 10 0.3836019461765261 1102 11 0.39078354938047255 1102 12 0.5048544226333789 1102 13 0.5339065944638719 1102 14 0.5678758552915895 1102 15 0.6885360870429835 1102 16 0.5875212588985121 1102 17 0.734877349251299 1102 18 0.9014312687629626 1102 19 0.7566227738052049 1102 20 0.7995183658932277 1102 21 0.6484221946182487 1102 22 0.74818230793543 1102 23 0.6452384450942006 1102 24 0.758189029398082 1102 25 0.700325362106085 1103 1 0.36892021044535284 1103 2 0.2628713777496152 1103 3 0.43704841906787095 1103 4 0.4604241826045765 1103 5 0.4031528488285616 1103 6 0.5522082452954677 1103 7 0.5255388754179577 1103 8 0.5165669615157569 1103 9 0.6200685452286692 1103 10 0.8058026311495112 1103 11 0.5267308855323591 1103 12 0.7717108076282102 1103 13 0.6573720229258413 1103 14 0.7375485503465953 1103 15 0.8350385466444763 1103 16 0.9665424810846659 1103 17 0.6318525735891356 1103 18 0.8801275949097231 1103 19 0.9470423384737714 1103 20 0.6492154153921561 1103 21 0.9260747637432871 1103 22 1.110960259236066 1103 23 1.3357609326362834 1103 24 1.151516531002315 1103 25 1.213041015297172 1104 1 0.472156404316479 1104 2 0.4619935298609441 1104 3 0.5257348960500323 1104 4 0.49907773736931205 1104 5 0.8102577705331129 1104 6 0.7421088375614815 1104 7 0.6088741564822087 1104 8 0.724466757771971 1104 9 0.8641201750119905 1104 10 0.8588900406384568 1104 11 0.8445264459079027 1104 12 0.9114011785140121 1104 13 1.010170812082741 1104 14 1.153515091223241 1104 15 1.3286175456087292 1104 16 1.472524574870917 1104 17 1.4655721697877908 1104 18 1.38169273582416 1104 19 1.2680930580546104 1104 20 1.066873977940027 1104 21 1.2788269743065825 1104 22 1.166327181689887 1104 23 1.2257628291697031 1104 24 1.3199448924575492 1104 25 1.2699695727497797 1105 1 0.3188036106576426 1105 2 0.2749019320880457 1105 3 0.3099762923436771 1105 4 0.40941660328542184 1105 5 0.4164509514017425 1105 6 0.43197628430145585 1105 7 0.3620234883745894 1105 8 0.3854897552759695 1105 9 0.5160686597641515 1105 10 0.6077890893260829 1105 11 0.6621917377030044 1105 12 0.4542797053839749 1105 13 0.6748596604183632 1105 14 0.7444066954843649 1105 15 0.6868339569678525 1105 16 0.798340461797423 1105 17 0.3987738597427253 1105 18 0.7652468383362532 1105 19 0.8043737415734213 1105 20 0.8640417010196799 1105 21 0.8975527642733496 1105 22 1.0535130554334913 1105 23 0.8250049185244492 1105 24 0.9549759687929011 1105 25 0.8895678332483428 1106 1 -0.11682314393164717 1106 2 -0.011178607016002928 1106 3 0.1272179773775406 1106 4 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-0.8021145644104806 2315 11 -0.6642209874252103 2315 12 -0.773388039159272 2315 13 -0.6826210902078174 2315 14 -0.8665889709216685 2315 15 -0.9662969652792555 2315 16 -1.2012371121851118 2315 17 -0.8391705986905507 2315 18 -1.0499025182509456 2315 19 -1.087379839936574 2315 20 -1.1383490645819165 2315 21 -0.8379555384686128 2315 22 -0.888839211420482 2315 23 -0.8496571406615772 2315 24 -0.7042826155664059 2315 25 -0.7721805797550871 2316 1 -1.3992096241179048 2316 2 -1.5875420038881138 2316 3 -1.5299015863977452 2316 4 -1.6414086420060912 2316 5 -1.6707700853466747 2316 6 -1.6911984113713885 2316 7 -1.8223718041359191 2316 8 -1.6946441477195826 2316 9 -2.0007828424231775 2316 10 -2.136673383207267 2316 11 -2.3334243896921016 2316 12 -2.4779914942044243 2316 13 -2.4248947068703046 2316 14 -2.6624439465599927 2316 15 -2.5879682339527825 2316 16 -2.5366469804325504 2316 17 -2.5829847612862125 2316 18 -2.5018100983676987 2316 19 -2.366621570806064 2316 20 -2.434812277490707 2316 21 -2.5095959125506226 2316 22 -2.6315398814469333 2316 23 -2.30348758728152 2316 24 -2.4585423682809413 2316 25 -2.40343399907837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67796.0 3 3.0 4 1197.0 5 10486.0 6 12484.0 7 10040.0 8 8064.0 9 7120.0 10 6513.0 11 6274.0 12 5861.0 13 5734.0 14 5516.0 15 5473.0 16 5611.0 17 5967.0 18 6468.0 19 7301.0 20 8524.0 21 11261.0 22 14224.0 23 17664.0 24 21544.0 25 25245.0 26 28766.0 27 32610.0 28 38129.0 29 46359.0 30 57834.0 31 73356.0 32 92141.0 33 117324.0 34 149909.0 35 197049.0 36 278532.0 37 448615.0 38 604767.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.21601719125851 21.01924889789874 14.774631957997023 23.99010195284573 2 14.736085964187323 21.542198437930658 41.2954011469591 22.42631445092292 3 19.149499070547854 25.41571431438212 30.614789899584764 24.81999671548526 4 13.175859553822017 18.32312826685331 38.15995914424057 30.341053035084105 5 12.88291635618518 38.459326344956644 36.0642185636727 12.593538735185476 6 31.367566276961583 38.210332624691766 17.901096790390213 12.52100430795643 7 27.77642856938087 31.909797887672052 22.338386107403633 17.975387435543446 8 24.401159654855658 39.043010351955004 20.315051309280474 16.240778683908868 9 25.903026545186037 15.652883308399144 20.875384609714054 37.56870553670076 10 14.9996252704503 30.118099191526117 34.38010517818902 20.50217035983456 11 34.005334674441926 22.464074084236746 25.32139713919585 18.209194102125476 12 23.370960548554915 24.916975903866103 32.27265076311727 19.43941278446171 13 29.061976172115124 22.42615063472633 26.05635850519359 22.455514687964957 14 20.24215310179825 23.413102265127506 27.81594922680803 28.528795406266216 15 22.272859628767925 32.850512396585906 23.36342500351181 21.51320297113436 16 21.23299536686842 28.92604968299518 28.9799861657222 20.8609687844142 17 20.879193336284754 29.76372380425439 28.542883599172892 20.814199260287964 18 20.68351488945888 28.07670365772899 31.338120315624668 19.901661137187464 19 23.281762629512063 27.215767636554112 28.030998938880586 21.471470795053243 20 23.24060381011901 28.20259640480784 28.778574152015697 19.778225633057453 21 22.3190557962061 27.409193610676887 27.623588057963083 22.648162535153933 22 22.600532975995602 29.02020304198486 28.073714012141238 20.305549969878296 23 21.62836575733661 28.732664662921554 28.476210407161062 21.162759172580774 24 22.787488210353104 27.442612114781095 29.275633446516675 20.49426622834913 25 22.294355894002685 28.131367471204438 28.846182988349078 20.7280936464438 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 252.0 1 252.0 2 415.0 3 578.0 4 578.0 5 578.0 6 1470.0 7 2362.0 8 2362.0 9 2362.0 10 3546.5 11 4731.0 12 4731.0 13 4731.0 14 8105.0 15 11479.0 16 11479.0 17 11479.0 18 20985.0 19 30491.0 20 30491.0 21 30491.0 22 50263.0 23 70035.0 24 70035.0 25 70035.0 26 102673.5 27 135312.0 28 135312.0 29 135312.0 30 176680.5 31 218049.0 32 218049.0 33 218049.0 34 241177.0 35 264305.0 36 264305.0 37 264305.0 38 277633.0 39 290961.0 40 290961.0 41 290961.0 42 302673.0 43 314385.0 44 314385.0 45 314385.0 46 314367.0 47 314349.0 48 314349.0 49 314349.0 50 296965.5 51 279582.0 52 279582.0 53 279582.0 54 241589.5 55 203597.0 56 203597.0 57 203597.0 58 171179.5 59 138762.0 60 138762.0 61 138762.0 62 112230.5 63 85699.0 64 85699.0 65 85699.0 66 65224.5 67 44750.0 68 44750.0 69 44750.0 70 32537.0 71 20324.0 72 20324.0 73 20324.0 74 14127.0 75 7930.0 76 7930.0 77 7930.0 78 5322.0 79 2714.0 80 2714.0 81 2714.0 82 1773.0 83 832.0 84 832.0 85 832.0 86 527.5 87 223.0 88 223.0 89 223.0 90 134.5 91 46.0 92 46.0 93 46.0 94 28.0 95 10.0 96 10.0 97 10.0 98 6.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38341180811717446 2 0.0 3 0.0 4 0.0 5 0.027029672437228702 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006225015470392065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2441761.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.97709886067634 #Duplication Level Percentage of deduplicated Percentage of total 1 87.09328858774583 63.55815531369341 2 9.037294272947024 13.190310351797581 3 1.9897403013282842 4.356164240313184 4 0.71230668754527 2.0792830222444825 5 0.32573257182070803 1.1885509047951082 6 0.19405076093546297 0.849675693886605 7 0.1304571675112835 0.6664269927388324 8 0.08125979825653822 0.4744083464612803 9 0.06423575681758602 0.4218965258100605 >10 0.32737138561982243 4.207802167964918 >50 0.021776374850408856 1.1019591075369957 >100 0.017557294387973304 2.6723245502750443 >500 0.00240850829610339 1.1546596511591276 >1k 0.00224047283356511 2.8261120653126004 >5k 2.8005910419563875E-4 1.252271066010744 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6920 0.2834020200994282 No Hit CTGTAGGACGTGGAATATGGCAAGA 6657 0.2726311051736841 No Hit GTATCAACGCAGAGTACTTTTTTTT 5990 0.24531475439242417 No Hit CTTTAGGACGTGAAATATGGCGAGG 5643 0.23110369933830543 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5426 0.22221667067333783 No Hit GGTATCAACGCAGAGTACTTTTTTT 4165 0.17057361469857205 No Hit TATCAACGCAGAGTACTTTTTTTTT 4148 0.1698773958630677 No Hit CTGAAGGACCTGGAATATGGCGAGA 3200 0.13105295727141192 No Hit GTCCTACAGTGTGCATTTCTCATTT 2692 0.11024830030457528 No Hit TTGTAGAACAGTGTATATCAATGAG 2562 0.10492427391542417 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.095404914731622E-5 0.0 8 0.0 0.0 0.0 4.095404914731622E-5 0.0 9 0.0 0.0 0.0 8.190809829463245E-5 0.0 10 0.0 0.0 0.0 1.2286214744194866E-4 0.0 11 0.0 0.0 0.0 1.2286214744194866E-4 0.0 12 0.0 0.0 0.0 1.2286214744194866E-4 0.0 13 0.0 4.095404914731622E-5 0.0 1.2286214744194866E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 990 0.0 12.666708 4 TCCAACG 320 0.0 12.168674 18 GGCGAGG 1490 0.0 11.920416 19 GGTATCA 1795 0.0 11.844967 1 CCAACGA 205 0.0 11.583031 19 AGCGTGC 75 2.0798143E-4 11.397001 8 AATGTCC 690 0.0 11.149241 8 TATGTCG 210 0.0 10.854288 16 TGTAGGA 3485 0.0 10.794869 2 CACCTTT 1075 0.0 10.690211 14 AAATGTC 695 0.0 10.659066 7 ACCTTTT 1070 0.0 10.47388 15 CTGTAGG 3510 0.0 10.457962 1 ACTGTTC 1005 0.0 10.395275 8 CTAGGAC 330 0.0 10.363669 3 GTAGGAC 3500 0.0 10.260033 3 GGACGTG 3545 0.0 10.207471 6 AGGACGT 3590 0.0 10.1615925 5 ATGTCGA 245 0.0 10.078982 17 CCACCTT 1075 0.0 10.07177 13 >>END_MODULE