FastQCFastQC Report
Thu 26 May 2016
SRR1512248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058339
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT64520.3134566269210271No Hit
CTGTAGGACGTGGAATATGGCAAGA53900.2618616272635363No Hit
GTCCTAAAGTGTGTATTTCTCATTT50790.24675235711901683No Hit
CTTTAGGACGTGAAATATGGCGAGG45600.22153785163668374No Hit
GTATCAACGCAGAGTACTTTTTTTT43810.21284151930269993No Hit
GTCCTACAGTGTGCATTTCTCATTT26940.13088223076956712No Hit
GGTATCAACGCAGAGTACTTTTTTT26710.12976482493894348No Hit
TATCAACGCAGAGTACTTTTTTTTT25780.12524661875424797No Hit
CTGAAGGACCTGGAATATGGCGAGA22590.10974868571212032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAT400.00554953714.1433786
CGTTTCC1650.013.71545714
CGGTAGG1151.9936124E-913.2362261
CCGTGCA500.001587431513.2001669
CCGTTTC1650.013.1439813
TCCAACG2900.012.68180918
TGGACCG604.360645E-412.5718925
ACAGCGC604.3615382E-412.5715878
TAGGACC9650.012.5067534
CCAACGA1800.012.04952619
TTAGGAC17900.011.7993163
GCTCCGT658.5470814E-411.6048226
GGCGAGG14000.011.5181519
CCCGTTT1900.011.41450912
GTCTAGG2050.011.3853051
GACAGCG752.2242486E-411.3147037
AATCCCG4000.011.08032419
AGGACGT34200.010.9452655
TTTAGGA19300.010.936462
GGACGTG33450.010.8242296
Produced by FastQC (version 0.11.5)