FastQCFastQC Report
Thu 26 May 2016
SRR1512248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058339
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60290.29290607620999265No Hit
CTGTAGGACGTGGAATATGGCAAGA57100.277408143167865No Hit
GTCCTACAGTGGACATTTCTAAATT54070.26268753592095373No Hit
CTTTAGGACGTGAAATATGGCGAGG49840.24213698520991925No Hit
GTCCTAAAGTGTGTATTTCTCATTT45970.22333541753812178No Hit
TATCAACGCAGAGTACTTTTTTTTT41390.2010844666500513No Hit
GGTATCAACGCAGAGTACTTTTTTT39410.19146505993424795No Hit
CTGAAGGACCTGGAATATGGCGAGA25900.1258296131006603No Hit
GTCCTACAGTGTGCATTTCTCATTT23870.11596729207385179No Hit
TTGTAGAACAGTGTATATCAATGAG21070.10236409065756417No Hit
TTTCTAAATTTTCCACCTTTTTCAG20950.10178109631115186No Hit
CTGTAGGACCTGGAATATGGCGAGA20680.10046935903172412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9000.013.5113014
AGTGCGA604.101778E-412.66346110
TCCAACG2100.012.6634618
CCAACGT802.8748585E-511.87199319
GGTATCA15800.011.1020221
TCGATTT600.005887839511.08052813
GATATAC9450.010.9960341
AGGACCT18550.010.8572965
ACTGTTC9450.010.8543948
GGCGAGG13800.010.66758819
CACCTTT7950.010.63252714
TGTAGAA10100.010.6288622
CTTACAC1803.45608E-1110.5557043
AGTCGTC2250.010.5528848
TGTAGGA31650.010.5056762
GGACCTG17850.010.37552456
CCAACGA1752.3646862E-1010.31167419
CTGTAGG31750.010.2688961
TAGGACA8250.010.2486294
GTATTAA2053.6379788E-1210.2308141
Produced by FastQC (version 0.11.5)