FastQCFastQC Report
Thu 26 May 2016
SRR1512247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884733
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70970.24601930230631397No Hit
CTGTAGGACGTGGAATATGGCAAGA61500.21319130747975637No Hit
GTCCTAAAGTGTGTATTTCTCATTT52880.18330985917934173No Hit
GTATCAACGCAGAGTACTTTTTTTT50080.17360358826969427No Hit
CTTTAGGACGTGAAATATGGCGAGG48910.1695477536395916No Hit
TATCAACGCAGAGTACTTTTTTTTT29210.10125720473957209No Hit
GGTATCAACGCAGAGTACTTTTTTT29090.10084122170058721No Hit
GTCCTACAGTGTGCATTTCTCATTT28920.1002519123953586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10050.013.1277764
GTCAACG751.0718802E-412.2382
GGACCGT1053.022269E-711.6676486
GCACCGT1552.910383E-1111.551846
CGGTAGG1258.414645E-811.4221341
GGCGAGG16550.011.27545519
GGTATCA18300.011.2571771
TCCAACG3350.011.25335618
TGTAGGA31000.010.8562912
GTCCTAC33550.010.7606871
GTAGGAC31750.010.6853323
GATATAC11000.010.6618951
ACTGTTC13000.010.6558528
AATCCCG5500.010.62419119
AGACCGC804.065156E-410.60148510
CCTAGTA1551.3524186E-810.5273122
AGGACGT35900.010.5001335
GGACGTG35850.010.4359176
AGTACCG1002.623105E-510.3662565
GTATAAG3250.010.3552311
Produced by FastQC (version 0.11.5)