FastQCFastQC Report
Thu 26 May 2016
SRR1512247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884733
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT75580.2619999840539835No Hit
CTGTAGGACGTGGAATATGGCAAGA69020.23925957792280947No Hit
GTATCAACGCAGAGTACTTTTTTTT64540.22372954446737361No Hit
CTTTAGGACGTGAAATATGGCGAGG57010.19762660877107172No Hit
GTCCTAAAGTGTGTATTTCTCATTT56450.1956853545891422No Hit
TATCAACGCAGAGTACTTTTTTTTT41000.14212753831983757No Hit
GGTATCAACGCAGAGTACTTTTTTT40790.14139956800161402No Hit
GTCCTACAGTGTGCATTTCTCATTT31510.1092302129867825No Hit
CTGAAGGACCTGGAATATGGCGAGA28890.10014791663561237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3800.012.49649918
TAGGACC11050.011.9508144
GGCGAGG14050.011.4922419
CCAACGT2000.010.92269819
GTAAGAC2450.010.8576783
GTCCTAG4100.010.6976421
ACTGTTC12350.010.6893148
GGACGTA803.7825614E-410.6845066
GATATAC10700.010.6041841
AGGACGT35700.010.32543855
TTAGGAC21650.010.2684053
GGACGTG34950.010.135936
TGTAGGA35050.010.1103422
GACGTGA18200.010.0713557
ACTGCGT856.6135987E-410.0560068
GTCCTAC33800.010.0426271
CCTACAC4100.09.9639053
CTGTAGG35150.09.9553111
GTAGGAC34750.09.9242313
TAAGACT2900.09.8280714
Produced by FastQC (version 0.11.5)