FastQCFastQC Report
Thu 26 May 2016
SRR1512246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586412
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70080.2709545115008746No Hit
CTGTAGGACGTGGAATATGGCAAGA61990.23967565878908695No Hit
GTATCAACGCAGAGTACTTTTTTTT53950.20859012407922636No Hit
GTCCTAAAGTGTGTATTTCTCATTT52980.2048397548418427No Hit
CTTTAGGACGTGAAATATGGCGAGG46740.18071366820135384No Hit
GGTATCAACGCAGAGTACTTTTTTT33670.13018034249763766No Hit
TTGTAGAACAGTGTATATCAATGAG31920.12341421243019288No Hit
TATCAACGCAGAGTACTTTTTTTTT30810.11912255278741361No Hit
GATATACACTGTTCTACAAATCCCG27300.10555162905213863No Hit
GTCCTACAGTGTGCATTTCTCATTT27100.10477835704443067No Hit
GTGTATATCAATGAGTTACAATGAA26130.10102798780704698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4400.013.92699818
GTCAACG757.3128285E-613.5630762
GACTCCG852.9687908E-713.3081367
CCAACGA3050.012.67304119
TAGGACC10350.012.2044824
GTCGAGG1900.011.9084319
TCGGTCA1002.119792E-611.311913515
CCAACGT1950.011.11962319
TGTAGAA14550.010.9763042
GGCGAGG13650.010.91242519
GGTATCA14700.010.79511
TGTAGGA33000.010.7579842
TGTCGAG2550.010.72147818
AGGACGT33600.010.6610315
GTATTAG2400.010.5961511
AGGACCT21750.010.5317825
GGACGTG33300.010.5306116
CTGTAGG32150.010.4412471
TATGTCG2350.010.42982816
GTAGGAC34850.010.4138863
Produced by FastQC (version 0.11.5)