##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512246_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2586412 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.38047457249657 31.0 30.0 34.0 25.0 34.0 2 26.509316381148867 31.0 26.0 34.0 2.0 34.0 3 28.16970227481159 31.0 27.0 34.0 16.0 34.0 4 32.71001642429744 35.0 32.0 37.0 26.0 37.0 5 33.123017910526244 35.0 33.0 37.0 27.0 37.0 6 33.33511288998041 36.0 35.0 37.0 28.0 37.0 7 33.33501971070348 37.0 35.0 37.0 28.0 37.0 8 33.34056291109073 37.0 35.0 37.0 28.0 37.0 9 34.84895097919434 39.0 35.0 39.0 28.0 39.0 10 34.70246001023812 39.0 35.0 39.0 27.0 39.0 11 34.72664950518324 39.0 35.0 39.0 27.0 39.0 12 34.60427920996346 38.0 35.0 39.0 27.0 39.0 13 34.608902603297544 39.0 35.0 39.0 27.0 39.0 14 35.65401490559122 39.0 36.0 41.0 27.0 41.0 15 35.59181097211117 39.0 36.0 41.0 27.0 41.0 16 35.54067913387349 39.0 36.0 41.0 27.0 41.0 17 35.522047918119775 39.0 36.0 41.0 27.0 41.0 18 35.47483192932913 39.0 36.0 41.0 27.0 41.0 19 35.49904771552251 40.0 36.0 41.0 27.0 41.0 20 35.41046244759149 39.0 36.0 41.0 27.0 41.0 21 35.33177080836309 39.0 36.0 41.0 26.0 41.0 22 35.22386417941148 39.0 35.0 41.0 26.0 41.0 23 35.10793910637594 39.0 35.0 41.0 25.0 41.0 24 34.99224369512668 39.0 35.0 41.0 25.0 41.0 25 34.865906127871355 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.33578762615869806 1101 2 3.3521424285980785 1101 3 1.8164559817265378 1101 4 0.8404041871293302 1101 5 0.4000519952628494 1101 6 0.34772230537623017 1101 7 0.12058086130791423 1101 8 0.07972826062324145 1101 9 0.006549630428935416 1101 10 -0.024378258908697603 1101 11 0.13635114363246714 1101 12 0.0444430848694779 1101 13 0.14540171266155966 1101 14 0.20759191668177834 1101 15 0.11335946670782704 1101 16 0.3286876983805129 1101 17 0.09799088174332837 1101 18 0.16018002591071223 1101 19 -0.051844239910934675 1101 20 0.15354976621622285 1101 21 0.21834834414048032 1101 22 0.16480378942921448 1101 23 0.1873808801601271 1101 24 0.10194970417005322 1101 25 0.17571256673856084 1102 1 1.1140796223245246 1102 2 4.025702367490961 1102 3 2.4094009553666034 1102 4 1.403252407833854 1102 5 0.8778744551238589 1102 6 0.9558766607560543 1102 7 0.8840341622904191 1102 8 0.6582239471796782 1102 9 0.6606092098681913 1102 10 0.7243336989154088 1102 11 0.7511104594723861 1102 12 0.7285095538366448 1102 13 0.6485394433109732 1102 14 0.8942269813834329 1102 15 0.9535935608846771 1102 16 0.7792366140287328 1102 17 0.9128572848106629 1102 18 0.969658368829478 1102 19 0.8879809757612449 1102 20 0.9044446559837738 1102 21 0.99989040021525 1102 22 0.7506607867932189 1102 23 0.842189321329549 1102 24 0.936885601605951 1102 25 0.8109045748861803 1103 1 0.972299646007265 1103 2 3.8095376379569537 1103 3 2.273883934299537 1103 4 1.2961066340976757 1103 5 1.0510559763434486 1103 6 0.7811507175898171 1103 7 0.6652336473229781 1103 8 0.6683758793884422 1103 9 0.5716317108709816 1103 10 0.6890407751541261 1103 11 0.5490598970674156 1103 12 0.6752519163939041 1103 13 0.6714535729703641 1103 14 0.6872957573990135 1103 15 0.8842017580903061 1103 16 0.8543716608777956 1103 17 0.9968094077303178 1103 18 0.8688911417994518 1103 19 0.9620669926177783 1103 20 0.914382518820851 1103 21 1.0698967892702669 1103 22 1.1145120659252683 1103 23 1.0976209188094757 1103 24 1.2846753728729823 1103 25 1.1803973175441698 1104 1 1.1082290687991012 1104 2 3.8501170426518456 1104 3 2.3180081768881635 1104 4 1.5356143318961344 1104 5 1.0694529098519112 1104 6 0.9108216495633741 1104 7 0.8405632830096295 1104 8 0.6737656870568642 1104 9 0.707031416280536 1104 10 0.8599007166332484 1104 11 0.6652273340030348 1104 12 0.788738455732755 1104 13 0.929074967031184 1104 14 1.0374451720865068 1104 15 0.6622547695499321 1104 16 0.8706320873729112 1104 17 0.7901155823022847 1104 18 0.8395327341885945 1104 19 0.9181751498208257 1104 20 0.907353314407743 1104 21 1.0415413468317567 1104 22 1.0086591483148482 1104 23 0.9406197416926716 1104 24 1.1032639176382517 1104 25 0.9857384813276298 1105 1 0.7480265397273982 1105 2 3.613748840802895 1105 3 2.2523984745174346 1105 4 1.232193359836053 1105 5 0.7713293216598558 1105 6 0.5912036688708895 1105 7 0.30853751901191373 1105 8 0.4271534418250198 1105 9 0.4962754409659169 1105 10 0.5256373283409275 1105 11 0.4741986905890556 1105 12 0.27027919468164896 1105 13 0.45775314668852474 1105 14 0.5758686975250527 1105 15 0.5343430708696246 1105 16 0.3366311069223258 1105 17 0.42059336790963897 1105 18 0.6463158980173347 1105 19 0.669449647938805 1105 20 0.7018471027449422 1105 21 0.6280494586288299 1105 22 0.7328202144935076 1105 23 0.4994510130414298 1105 24 0.5813891477052451 1105 25 0.5809786216056807 1106 1 -0.03812942714272083 1106 2 2.8125519228207203 1106 3 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0.4601631751274837 1107 15 0.42570000539552666 1107 16 0.529084628949029 1107 17 0.2446935221752966 1107 18 0.3919197179074345 1107 19 0.37905335500609283 1107 20 0.5856726954277676 1107 21 0.5289418759302649 1107 22 0.5389318320040601 1107 23 0.6688537726204729 1107 24 0.7339271304556405 1107 25 0.7920910545682673 1108 1 0.8816048568900392 1108 2 3.454680630837288 1108 3 2.0968660711907 1108 4 1.1979709004461796 1108 5 0.8310495336959747 1108 6 0.5363342433243758 1108 7 0.6015183701880247 1108 8 0.4800880827368488 1108 9 0.472034561471915 1108 10 0.43715191452579205 1108 11 0.5276930646255238 1108 12 0.5365727901445041 1108 13 0.5081350227882027 1108 14 0.6837844860413682 1108 15 0.6352120888271173 1108 16 0.47140426061707075 1108 17 0.6742065097921639 1108 18 0.5156895134217265 1108 19 0.6044553407110058 1108 20 0.6466124751948428 1108 21 0.4934530110534254 1108 22 0.4918645080788693 1108 23 0.6425210734387079 1108 24 0.6729710590619291 1108 25 0.687613396022563 1109 1 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-1.6990802351723175 2315 18 -1.675980111881671 2315 19 -2.009775403637839 2315 20 -1.8238111879605938 2315 21 -1.8345735686154612 2315 22 -1.5924554407530422 2315 23 -1.6876694101479401 2315 24 -1.6333484057703629 2315 25 -1.7250534968112632 2316 1 -1.278551004272746 2316 2 0.09608963034842333 2316 3 -0.21414867434593177 2316 4 -0.9600501925728082 2316 5 -1.1401152663677365 2316 6 -1.083403423934378 2316 7 -1.2365774182867426 2316 8 -1.265302831039584 2316 9 -1.2432944314473104 2316 10 -1.3045582834749538 2316 11 -1.901388281363694 2316 12 -1.136610672999403 2316 13 -1.1651742507961345 2316 14 -1.422808340496175 2316 15 -1.500751254158132 2316 16 -1.415523398656184 2316 17 -1.4310471959474498 2316 18 -1.798889815176345 2316 19 -1.8145951803377756 2316 20 -1.9619044215620676 2316 21 -1.7720458674363613 2316 22 -1.8948029586131625 2316 23 -1.6917334872588796 2316 24 -1.4532092087732877 2316 25 -1.2939394753311433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94072.0 3 4.0 4 864.0 5 4268.0 6 4913.0 7 4615.0 8 4252.0 9 4057.0 10 3853.0 11 3776.0 12 3797.0 13 3749.0 14 3709.0 15 3987.0 16 4160.0 17 4437.0 18 5211.0 19 6360.0 20 8260.0 21 10783.0 22 13246.0 23 15919.0 24 18822.0 25 21345.0 26 24019.0 27 27788.0 28 32613.0 29 39751.0 30 50550.0 31 64586.0 32 84037.0 33 112328.0 34 152887.0 35 213915.0 36 305900.0 37 389680.0 38 839899.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23600107020846 19.274655391329766 13.729908460059729 24.75943507840205 2 15.101119863952448 20.60981913869649 42.004390003542206 22.284670993808852 3 19.08485577703784 24.2050763760762 29.75148584216281 26.958582004723148 4 11.927682055295135 16.88021861946202 38.90196148177475 32.290137843468095 5 12.79204550551111 38.022287245806154 36.1508529963517 13.034814252331028 6 31.211843770854227 37.64095814810204 18.31475800783017 12.832440073213563 7 27.583445563328844 31.682034732718613 22.144631114214754 18.58988858973779 8 23.977734405810054 38.88518147920749 20.60796965062024 16.529114464362213 9 25.6352429543321 16.11351942381956 20.83797940931298 37.413258212535354 10 14.703405493269903 29.772778943754226 34.669480476295796 20.85433508668008 11 33.8109849694587 22.704724138491006 25.172038878195934 18.312252013854362 12 23.175307221443795 24.58598952678773 32.52789196445418 19.710811287314296 13 28.766182649941307 22.424810896330516 26.24987820965878 22.55912824406939 14 20.08151085445275 23.210256402325392 27.930980620196998 28.777252123024866 15 22.23520460004052 32.45851782314651 23.582553746270897 21.72372383054208 16 20.985055745179036 28.863189623308273 29.22600884932486 20.925745782187835 17 20.57915047465424 29.371998009595547 28.90768629400841 21.141165221741804 18 20.263090530705238 28.261293233920032 31.533587563696152 19.942028671678578 19 22.82720529243118 27.164153135614683 28.01659491133206 21.992046660622076 20 22.663017338304954 28.2837382443323 29.047189697542386 20.006054719820355 21 22.10664496209756 27.53331567581571 27.636592820865776 22.72344654122096 22 21.966036450441372 29.138546397815308 28.324622896803657 20.570794254939663 23 21.56856300957844 28.474296966859203 28.530940959174185 21.426199064388175 24 22.470289537323392 27.241093434735973 29.548792286222714 20.73982474171792 25 22.051981817422547 27.99712475234049 29.0776626200791 20.873230810157867 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 242.0 1 242.0 2 371.0 3 500.0 4 500.0 5 500.0 6 1327.5 7 2155.0 8 2155.0 9 2155.0 10 3401.5 11 4648.0 12 4648.0 13 4648.0 14 8238.5 15 11829.0 16 11829.0 17 11829.0 18 21579.5 19 31330.0 20 31330.0 21 31330.0 22 51321.0 23 71312.0 24 71312.0 25 71312.0 26 105414.5 27 139517.0 28 139517.0 29 139517.0 30 185219.0 31 230921.0 32 230921.0 33 230921.0 34 254379.5 35 277838.0 36 277838.0 37 277838.0 38 292902.0 39 307966.0 40 307966.0 41 307966.0 42 321274.5 43 334583.0 44 334583.0 45 334583.0 46 335167.5 47 335752.0 48 335752.0 49 335752.0 50 316220.5 51 296689.0 52 296689.0 53 296689.0 54 258732.5 55 220776.0 56 220776.0 57 220776.0 58 185772.5 59 150769.0 60 150769.0 61 150769.0 62 121021.0 63 91273.0 64 91273.0 65 91273.0 66 69171.5 67 47070.0 68 47070.0 69 47070.0 70 33588.0 71 20106.0 72 20106.0 73 20106.0 74 13815.5 75 7525.0 76 7525.0 77 7525.0 78 5012.5 79 2500.0 80 2500.0 81 2500.0 82 1647.0 83 794.0 84 794.0 85 794.0 86 502.0 87 210.0 88 210.0 89 210.0 90 143.0 91 76.0 92 76.0 93 76.0 94 51.5 95 27.0 96 27.0 97 27.0 98 15.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.330734623872763 3 0.0 4 0.0 5 0.0 6 2.319816023123926E-4 7 0.007926038079006747 8 0.0 9 0.0 10 0.004639632046247852 11 0.003015760830061104 12 1.5465440154159509E-4 13 0.0 14 3.0930880308319017E-4 15 0.0 16 0.0 17 1.159908011561963E-4 18 0.011831061717932023 19 0.0029770972296757053 20 0.0 21 0.006456821264361594 22 0.001971843619655337 23 0.003209078831988098 24 0.011057789710224048 25 0.007732720077079754 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2586412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.23702426596971 #Duplication Level Percentage of deduplicated Percentage of total 1 87.82779581857221 66.07902005228551 2 8.828611689053757 13.284769437683227 3 1.7415758677474296 3.930929574682219 4 0.6154890390348738 1.8523025506122073 5 0.28692297019786867 1.0793615235620575 6 0.16185287444324045 0.7306397185201818 7 0.09966688940098954 0.5249048123463179 8 0.06687475428374111 0.40251660086612734 9 0.05847113887515323 0.3959275044967909 >10 0.2714981116528252 3.619194845530558 >50 0.020275661598661756 1.0758143539465552 >100 0.01701037586836998 2.5884120429776876 >500 0.0020814785636180514 1.1662318735774992 >1k 0.0016651828508790596 2.3342646960514424 >5k 2.0814785635988245E-4 0.9357104128615188 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7008 0.2709545115008746 No Hit CTGTAGGACGTGGAATATGGCAAGA 6199 0.23967565878908695 No Hit GTATCAACGCAGAGTACTTTTTTTT 5395 0.20859012407922636 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5298 0.2048397548418427 No Hit CTTTAGGACGTGAAATATGGCGAGG 4674 0.18071366820135384 No Hit GGTATCAACGCAGAGTACTTTTTTT 3367 0.13018034249763766 No Hit TTGTAGAACAGTGTATATCAATGAG 3192 0.12341421243019288 No Hit TATCAACGCAGAGTACTTTTTTTTT 3081 0.11912255278741361 No Hit GATATACACTGTTCTACAAATCCCG 2730 0.10555162905213863 No Hit GTCCTACAGTGTGCATTTCTCATTT 2710 0.10477835704443067 No Hit GTGTATATCAATGAGTTACAATGAA 2613 0.10102798780704698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 3.866360038539877E-5 0.0 0.0 0.0 0.0 12 3.866360038539877E-5 0.0 0.0 0.0 0.0 13 3.866360038539877E-5 7.732720077079754E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 440 0.0 13.926998 18 GTCAACG 75 7.3128285E-6 13.563076 2 GACTCCG 85 2.9687908E-7 13.308136 7 CCAACGA 305 0.0 12.673041 19 TAGGACC 1035 0.0 12.204482 4 GTCGAGG 190 0.0 11.90843 19 TCGGTCA 100 2.119792E-6 11.3119135 15 CCAACGT 195 0.0 11.119623 19 TGTAGAA 1455 0.0 10.976304 2 GGCGAGG 1365 0.0 10.912425 19 GGTATCA 1470 0.0 10.7951 1 TGTAGGA 3300 0.0 10.757984 2 TGTCGAG 255 0.0 10.721478 18 AGGACGT 3360 0.0 10.661031 5 GTATTAG 240 0.0 10.596151 1 AGGACCT 2175 0.0 10.531782 5 GGACGTG 3330 0.0 10.530611 6 CTGTAGG 3215 0.0 10.441247 1 TATGTCG 235 0.0 10.429828 16 GTAGGAC 3485 0.0 10.413886 3 >>END_MODULE