FastQCFastQC Report
Thu 26 May 2016
SRR1512246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586412
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72120.27884188597949594No Hit
GTCCTACAGTGGACATTTCTAAATT70340.271959765110895No Hit
CTGTAGGACGTGGAATATGGCAAGA67500.2609793026014417No Hit
CTTTAGGACGTGAAATATGGCGAGG53230.20580634485147764No Hit
GTCCTAAAGTGTGTATTTCTCATTT50950.19699104396360673No Hit
GGTATCAACGCAGAGTACTTTTTTT46030.17796855257399055No Hit
TATCAACGCAGAGTACTTTTTTTTT45720.17676998096204316No Hit
TTGTAGAACAGTGTATATCAATGAG39540.15287587592386673No Hit
GATATACACTGTTCTACAAATCCCG33070.1278605264745137No Hit
CTGAAGGACCTGGAATATGGCGAGA30630.11842660798047644No Hit
GTCCTACAGTGTGCATTTCTCATTT26940.10415973943826429No Hit
GTGTATATCAATGAGTTACAATGAA25970.1004093702008806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAG456.7778496E-414.7739310
CCAACGA3650.012.75108919
TCCAACG5150.012.35600518
TAGGACC9700.012.24191954
GATATAC13700.011.4839141
TACACCG1001.9316813E-611.3996755
ACTGTTC14400.011.2123578
GGTATCA19400.010.8621841
GTTCTAG2900.010.8503191
ACACGGT803.7815917E-410.6847166
TGTAGGA32150.010.6077842
GTCCTAC34500.010.4748231
GTAGGAC31950.010.2876573
GGCGAGG15550.010.261785519
CACTGTT15100.010.25229557
TATACAC17000.010.2261793
CGTTTCC4000.010.2098414
TAGACTG3350.010.2086655
AGGACCT21900.010.1937745
CCGTTTC4200.010.175920513
Produced by FastQC (version 0.11.5)