##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512246_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2586412 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.136794524615567 31.0 30.0 34.0 26.0 34.0 2 30.349103700415867 31.0 30.0 34.0 26.0 34.0 3 30.423187798386337 31.0 30.0 34.0 26.0 34.0 4 34.03000914007513 37.0 35.0 37.0 30.0 37.0 5 33.55224496329278 35.0 35.0 37.0 28.0 37.0 6 33.469585278756824 36.0 35.0 37.0 28.0 37.0 7 33.2568917867687 36.0 35.0 37.0 27.0 37.0 8 33.13864109816998 35.0 33.0 37.0 27.0 37.0 9 34.52295612609283 38.0 35.0 39.0 27.0 39.0 10 34.276476833543924 38.0 34.0 39.0 27.0 39.0 11 34.265751937433016 38.0 34.0 39.0 27.0 39.0 12 34.042522614339866 37.0 34.0 39.0 26.0 39.0 13 34.02474470424666 37.0 34.0 39.0 26.0 39.0 14 34.87659004056585 38.0 34.0 40.0 25.0 41.0 15 34.799562482698036 38.0 34.0 40.0 25.0 41.0 16 34.676924635363584 38.0 34.0 40.0 25.0 41.0 17 34.592782201752854 38.0 34.0 40.0 25.0 41.0 18 34.51613354716882 38.0 33.0 40.0 24.0 41.0 19 34.535723620212096 38.0 34.0 40.0 24.0 41.0 20 34.38877487422731 38.0 34.0 40.0 23.0 41.0 21 34.25425028959037 38.0 34.0 40.0 22.0 41.0 22 34.33632422058048 38.0 34.0 40.0 22.0 41.0 23 34.20235291206505 38.0 34.0 40.0 19.0 41.0 24 34.042270914301355 38.0 34.0 40.0 18.0 41.0 25 33.54775109301998 38.0 33.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2079865294588501 1101 2 0.26335145201420573 1101 3 0.2895877704096961 1101 4 0.36214644918270267 1101 5 0.2686637514824355 1101 6 0.38319704418176315 1101 7 0.2459079825859476 1101 8 0.11982039899271513 1101 9 0.18963470691027595 1101 10 0.3714142285284936 1101 11 0.27895871526321514 1101 12 0.2629198051637829 1101 13 0.19891321887762814 1101 14 0.1215554260169256 1101 15 0.039987948066873 1101 16 0.20322357632893784 1101 17 0.3101298292800081 1101 18 0.2681916941434892 1101 19 0.3234646126751599 1101 20 0.19310836077924165 1101 21 -0.03607689275938242 1101 22 0.07170985406682462 1101 23 0.165926685452483 1101 24 -0.06658359331130725 1101 25 0.11650865299602486 1102 1 0.37282597350390034 1102 2 0.4935178490906509 1102 3 0.2927591998369685 1102 4 0.4228342037956878 1102 5 0.5066407764045522 1102 6 0.5577230147065677 1102 7 0.4665713243065355 1102 8 0.6491271028029217 1102 9 0.7523331552687722 1102 10 0.6395254494726572 1102 11 0.835711201484159 1102 12 0.9983095128081629 1102 13 0.7462637460762878 1102 14 0.7775395801027187 1102 15 1.0967629001397228 1102 16 0.8835419923366103 1102 17 0.868668350727404 1102 18 1.1428734279081638 1102 19 1.010841050489681 1102 20 1.180302825193337 1102 21 1.0510157264594042 1102 22 1.080033452197661 1102 23 1.1393121395622359 1102 24 1.0976230625717491 1102 25 1.0719401471249483 1103 1 0.4132259379557759 1103 2 0.30853368962367966 1103 3 0.39089064143355756 1103 4 0.4368108621277642 1103 5 0.3407009848414688 1103 6 0.367337281514466 1103 7 0.2925167122975125 1103 8 0.4154462144840849 1103 9 0.5608596614404533 1103 10 0.564584055842019 1103 11 0.28148890079985733 1103 12 0.417780998647757 1103 13 0.5836601941459989 1103 14 0.5041348622899875 1103 15 0.6972965423574493 1103 16 0.9392792916878534 1103 17 0.40726658965404283 1103 18 0.5985796826969505 1103 19 0.7409124830070937 1103 20 0.3677384242392989 1103 21 0.625492952840915 1103 22 0.9000356379270116 1103 23 0.8516548451108648 1103 24 0.4891802626284303 1103 25 0.6463516214834613 1104 1 0.3959874462659947 1104 2 0.32906905692947674 1104 3 0.3814121981445915 1104 4 0.35943299577720467 1104 5 0.500167259748487 1104 6 0.5004018729458508 1104 7 0.48291972732909727 1104 8 0.4547410471162152 1104 9 0.6275806337727232 1104 10 0.6315679600814477 1104 11 0.7140784362285189 1104 12 0.8375517884246619 1104 13 0.8604034371368812 1104 14 0.8364223965607991 1104 15 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-0.696181748119173 2315 5 -0.797423063346514 2315 6 -0.7951019694556223 2315 7 -0.8934846755793657 2315 8 -0.7912056516925787 2315 9 -0.9213110627118084 2315 10 -0.9597480615056497 2315 11 -0.9595605729806564 2315 12 -0.937141154638212 2315 13 -0.9257501876319267 2315 14 -1.1778536552204173 2315 15 -0.9464741761468218 2315 16 -1.1795596513488888 2315 17 -1.1024148664223574 2315 18 -0.9648892093578212 2315 19 -1.193636384937804 2315 20 -1.1738188983408833 2315 21 -1.1336131873817195 2315 22 -0.9437164304301859 2315 23 -1.2161702094496434 2315 24 -1.1384426349278414 2315 25 -1.0839422477660676 2316 1 -0.9714142842982518 2316 2 -1.1394895006957597 2316 3 -1.0028202021068076 2316 4 -0.9817235032201879 2316 5 -1.2844487734676662 2316 6 -1.2549425420304274 2316 7 -1.240760507357244 2316 8 -1.208909161117834 2316 9 -1.5308737953160403 2316 10 -1.5081511599253687 2316 11 -1.517810726622244 2316 12 -1.5947158849943648 2316 13 -1.623562022946416 2316 14 -1.8810859258946309 2316 15 -1.8481708305297744 2316 16 -1.8446278362305577 2316 17 -1.8624336975125644 2316 18 -1.7649079719090253 2316 19 -1.7207378477885271 2316 20 -1.6562122288108085 2316 21 -1.6436789962447236 2316 22 -1.7922185391467522 2316 23 -1.5585666355977281 2316 24 -1.4267790677470558 2316 25 -1.4871566126265776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61206.0 3 2.0 4 1121.0 5 9474.0 6 11742.0 7 9421.0 8 7625.0 9 6674.0 10 6497.0 11 5967.0 12 5589.0 13 5417.0 14 5333.0 15 5232.0 16 5369.0 17 5685.0 18 6253.0 19 7168.0 20 8240.0 21 10968.0 22 14045.0 23 17710.0 24 20995.0 25 24977.0 26 28514.0 27 32682.0 28 38322.0 29 46098.0 30 58366.0 31 74214.0 32 93589.0 33 120331.0 34 154530.0 35 202345.0 36 290297.0 37 476445.0 38 707969.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.62615348608481 20.732688539059293 14.806113422881051 23.835044551974846 2 14.480832906744942 21.205051631371955 41.793612154598726 22.520503307284375 3 19.281112212594127 25.102033241417065 30.486055585885 25.130798960103807 4 12.900264922989841 18.039082713813574 38.34145526698763 30.719197096208955 5 12.834835543299809 38.32938523805564 36.103007578409176 12.732771640235377 6 31.378604800781932 38.2024982872025 17.825002358479626 12.593894553535941 7 27.646136810376692 31.920243178580982 22.043897105333564 18.38972290570876 8 24.189417617920114 39.22457056339052 20.24824351263449 16.337768306054876 9 25.66594185303811 15.984421662132714 20.74402686037646 37.60560962445272 10 14.732996908458512 30.24239757625622 34.41211222341993 20.612493291865334 11 34.14703457917764 22.698510523458754 24.947146858273157 18.207308039090446 12 23.178209813440397 24.884937125253053 32.432033256882505 19.504819804424045 13 28.93139221438812 22.45191408020068 26.228458575045277 22.388235130365928 14 20.167977878234403 23.31067130836077 27.899770028904907 28.621580784499915 15 22.340524247490347 32.57922558354972 23.567706923722902 21.51254324523703 16 21.14090871833258 29.131592337183715 28.93398267561394 20.79351626886977 17 20.642805554567488 29.680344817453673 28.71360015341717 20.96324947456167 18 20.442489440970736 28.42551766694556 31.338665301583816 19.79332759049989 19 23.004958220113423 27.2491776252198 28.16047868630365 21.58538546836312 20 22.88498506811753 28.371272635604843 28.93232014079737 19.81142215548026 21 22.27475746323478 27.54197707093843 27.759382495905527 22.423882969921266 22 22.208410724973437 29.029907068170115 28.386970057361317 20.37471214949513 23 21.72596631936443 28.59702939825519 28.434719603837284 21.242284678543093 24 22.591876313595822 27.425328988575682 29.367594953936187 20.615199743892312 25 22.2072412403789 28.223035585996982 29.017740799198215 20.551982374425904 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 216.0 1 216.0 2 391.0 3 566.0 4 566.0 5 566.0 6 1459.5 7 2353.0 8 2353.0 9 2353.0 10 3683.0 11 5013.0 12 5013.0 13 5013.0 14 8609.0 15 12205.0 16 12205.0 17 12205.0 18 22102.0 19 31999.0 20 31999.0 21 31999.0 22 52985.5 23 73972.0 24 73972.0 25 73972.0 26 108021.0 27 142070.0 28 142070.0 29 142070.0 30 187519.5 31 232969.0 32 232969.0 33 232969.0 34 256996.0 35 281023.0 36 281023.0 37 281023.0 38 294623.0 39 308223.0 40 308223.0 41 308223.0 42 321823.0 43 335423.0 44 335423.0 45 335423.0 46 334745.5 47 334068.0 48 334068.0 49 334068.0 50 314452.0 51 294836.0 52 294836.0 53 294836.0 54 256396.5 55 217957.0 56 217957.0 57 217957.0 58 183036.5 59 148116.0 60 148116.0 61 148116.0 62 118596.5 63 89077.0 64 89077.0 65 89077.0 66 67613.5 67 46150.0 68 46150.0 69 46150.0 70 32877.0 71 19604.0 72 19604.0 73 19604.0 74 13391.0 75 7178.0 76 7178.0 77 7178.0 78 4824.0 79 2470.0 80 2470.0 81 2470.0 82 1589.0 83 708.0 84 708.0 85 708.0 86 438.5 87 169.0 88 169.0 89 169.0 90 105.5 91 42.0 92 42.0 93 42.0 94 23.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38597872264743593 2 0.0 3 0.0 4 0.0 5 0.030041617499454845 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006572812065517791 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2586412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.78118199580936 #Duplication Level Percentage of deduplicated Percentage of total 1 87.41725787521185 64.49748612865605 2 9.054877657393437 13.361591527798664 3 1.8851586071723196 4.172676908602423 4 0.6059500587426505 1.788308462578515 5 0.2947496730743176 1.0873489636150768 6 0.17084992877084598 0.7563305813167712 7 0.10370224456131005 0.5355891925546366 8 0.07946521109624793 0.4690429761782143 9 0.051371821070364344 0.34112463118638353 >10 0.2913611073544004 3.791645971500927 >50 0.022206222427683497 1.1562717286808601 >100 0.018605443891067247 2.846341024345885 >500 0.0019345120193494615 1.0006956927692046 >1k 0.0022482166710782086 2.9837249815798335 >5k 2.614205431486289E-4 1.2118212286365198 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7212 0.27884188597949594 No Hit GTCCTACAGTGGACATTTCTAAATT 7034 0.271959765110895 No Hit CTGTAGGACGTGGAATATGGCAAGA 6750 0.2609793026014417 No Hit CTTTAGGACGTGAAATATGGCGAGG 5323 0.20580634485147764 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5095 0.19699104396360673 No Hit GGTATCAACGCAGAGTACTTTTTTT 4603 0.17796855257399055 No Hit TATCAACGCAGAGTACTTTTTTTTT 4572 0.17676998096204316 No Hit TTGTAGAACAGTGTATATCAATGAG 3954 0.15287587592386673 No Hit GATATACACTGTTCTACAAATCCCG 3307 0.1278605264745137 No Hit CTGAAGGACCTGGAATATGGCGAGA 3063 0.11842660798047644 No Hit GTCCTACAGTGTGCATTTCTCATTT 2694 0.10415973943826429 No Hit GTGTATATCAATGAGTTACAATGAA 2597 0.1004093702008806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.866360038539877E-5 0.0 5 0.0 0.0 0.0 3.866360038539877E-5 0.0 6 0.0 0.0 0.0 3.866360038539877E-5 0.0 7 0.0 0.0 0.0 3.866360038539877E-5 0.0 8 0.0 0.0 0.0 3.866360038539877E-5 0.0 9 0.0 0.0 0.0 3.866360038539877E-5 0.0 10 0.0 0.0 0.0 7.732720077079754E-5 0.0 11 0.0 0.0 0.0 7.732720077079754E-5 0.0 12 0.0 0.0 0.0 7.732720077079754E-5 0.0 13 0.0 0.0 0.0 7.732720077079754E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCAG 45 6.7778496E-4 14.77393 10 CCAACGA 365 0.0 12.751089 19 TCCAACG 515 0.0 12.356005 18 TAGGACC 970 0.0 12.2419195 4 GATATAC 1370 0.0 11.483914 1 TACACCG 100 1.9316813E-6 11.399675 5 ACTGTTC 1440 0.0 11.212357 8 GGTATCA 1940 0.0 10.862184 1 GTTCTAG 290 0.0 10.850319 1 ACACGGT 80 3.7815917E-4 10.684716 6 TGTAGGA 3215 0.0 10.607784 2 GTCCTAC 3450 0.0 10.474823 1 GTAGGAC 3195 0.0 10.287657 3 GGCGAGG 1555 0.0 10.2617855 19 CACTGTT 1510 0.0 10.2522955 7 TATACAC 1700 0.0 10.226179 3 CGTTTCC 400 0.0 10.20984 14 TAGACTG 335 0.0 10.208665 5 AGGACCT 2190 0.0 10.193774 5 CCGTTTC 420 0.0 10.1759205 13 >>END_MODULE