FastQCFastQC Report
Thu 26 May 2016
SRR1512245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186286
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35250.161232336482967No Hit
GGTATCAACGCAGAGTACTTTTTTT22300.10199946393106848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC1650.014.2312641
ACACCGT1251.6043487E-912.0613556
CCCTATA708.167331E-411.6678692
ACTGTCG600.00624627110.992928
CGTGCGC804.0684524E-410.60031510
GTATAAT2600.010.2093841
CGAATTG750.00283282310.05135914
GTAGGAC3000.010.0511283
GCGTGCG857.110819E-49.9767679
TAGGACA2151.2732926E-119.6420724
GTACTAG1301.8700515E-59.42404651
GTCTTAG1954.802132E-99.42404651
TAGACTG2700.09.4229355
CTAGGAC2202.0008883E-119.4229343
CATTGCG800.00483227479.42250259
TGCGCAT800.00483227479.422502510
CCCCTAG1208.055246E-59.3586031
CTTACAC2750.09.2516083
GTTTAGG2007.41602E-99.1884461
GTACTAC1352.9195471E-59.0750081
Produced by FastQC (version 0.11.5)