FastQCFastQC Report
Thu 26 May 2016
SRR1512245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186286
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51650.2362453951587304No Hit
GGTATCAACGCAGAGTACTTTTTTT33440.1529534562266785No Hit
TATCAACGCAGAGTACTTTTTTTTT33250.1520844024981178No Hit
GTACATAAGCAGTGGTATCAACGCA21970.10049005482356836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGGT400.005289804714.2460214
ATACCGT600.00588918511.0802376
AACCGCC750.002654449810.1305047
ACTATAC1901.0004442E-109.9999523
GAACCGC951.6491584E-49.9972086
GTAGGAC2500.09.8799523
GTCTAGG2159.094947E-129.7590591
GTCCTAG2850.09.7045831
GTTCTAG2500.09.5372631
GTAAGAC1804.1836756E-99.4999543
ACTATCG800.0045271089.49734711
ACCGTAT900.00111674339.4973478
GGACCGC800.0045271089.4973476
TATACTG3400.09.2205445
GGTATCA19500.09.0481731
CAGTGCG1054.505872E-49.0450929
AGAACCG1701.6852937E-78.94113355
CTTACAC1701.6852937E-78.94113353
CCTATAC1502.7022434E-68.8666243
TAAGACC1502.7022434E-68.8666244
Produced by FastQC (version 0.11.5)