FastQCFastQC Report
Thu 26 May 2016
SRR1512244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389939
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39240.16418829099822213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG500.001592002613.195275
TAGGACC4200.011.6692864
ACCGGTC658.576332E-411.6002388
TCCAACG2250.011.31141418
TTAGACA2450.011.156354
GGCGAGG5450.010.37744419
GTCCTAC11250.010.2301241
GTCTAGG2301.8189894E-1210.1848591
GCGCCAC1304.2827196E-710.1504213
GTATCAA28100.010.112371
AATCCCG3400.09.980659519
TGGCGAG11050.09.980659518
GACGTGA5300.09.9586947
AGGACGT10250.09.9309345
TTAGGAC7050.09.8931093
GATATAC6900.09.8896471
GGACGTG10300.09.791226
ACTGTTC8250.09.7108058
TAGGACG10300.09.6997134
TGTAGGA11700.09.5755092
Produced by FastQC (version 0.11.5)