FastQCFastQC Report
Thu 26 May 2016
SRR1512244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389939
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52780.2208424566484751No Hit
GGTATCAACGCAGAGTACTTTTTTT35170.14715856764545035No Hit
TATCAACGCAGAGTACTTTTTTTTT33850.1416354141256325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG653.3833403E-614.6114747
TAGGACC4050.011.9625154
AACACCG752.0754256E-411.3995735
TCCAACG3000.011.3969518
ATAGGAC2700.011.2588383
TAGGACT3150.011.1583134
GATATAC7400.010.8252891
ACACCGT803.7816117E-410.6846416
GTCCTAC10650.010.47678851
GGTATCA15850.010.4691611
CACTGTT9550.010.3427827
AGGACGT9400.010.3081255
ATACACT10350.010.0962414
GAACAGT6450.010.0128246
ATTAGAC2002.5465852E-119.9746273
GTAGGAC9900.09.8834693
ACTGTTC9850.09.8349318
CCAACGT1451.6228114E-79.82557419
ACAGCGC1451.624303E-79.8249578
TGTAGAA9700.09.6955132
Produced by FastQC (version 0.11.5)