##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512244_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2389939 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.204983474473615 31.0 30.0 34.0 26.0 34.0 2 30.404215756134363 31.0 30.0 34.0 27.0 34.0 3 30.47696573008767 31.0 30.0 34.0 27.0 34.0 4 34.09671920496716 37.0 35.0 37.0 30.0 37.0 5 33.61550483087644 35.0 35.0 37.0 28.0 37.0 6 33.52903944410296 36.0 35.0 37.0 28.0 37.0 7 33.319703975708165 36.0 35.0 37.0 27.0 37.0 8 33.202803502516176 36.0 35.0 37.0 27.0 37.0 9 34.59869394156085 38.0 35.0 39.0 27.0 39.0 10 34.36433482193478 38.0 34.0 39.0 27.0 39.0 11 34.36316157023255 38.0 34.0 39.0 27.0 39.0 12 34.12640992092267 37.0 34.0 39.0 26.0 39.0 13 34.101509703804155 37.0 34.0 39.0 26.0 39.0 14 34.9767165605482 38.0 34.0 40.0 25.0 41.0 15 34.8792701403676 38.0 34.0 40.0 25.0 41.0 16 34.75491843097251 38.0 34.0 40.0 25.0 41.0 17 34.666169303902734 38.0 34.0 40.0 25.0 41.0 18 34.57920432278816 38.0 34.0 40.0 25.0 41.0 19 34.58714343755217 38.0 34.0 40.0 24.0 41.0 20 34.43292360181578 38.0 34.0 40.0 23.0 41.0 21 34.305414489658524 38.0 34.0 40.0 22.0 41.0 22 34.393958172154186 39.0 34.0 40.0 23.0 41.0 23 34.266147378656946 38.0 34.0 40.0 19.0 41.0 24 34.094340483167144 38.0 34.0 40.0 18.0 41.0 25 33.575886246469054 38.0 33.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26313478546801505 1101 2 0.2456380901794617 1101 3 0.38703699909180145 1101 4 0.3420903128153512 1101 5 0.396808267914615 1101 6 0.4372887535267509 1101 7 0.3063404951989739 1101 8 0.17675819872612664 1101 9 0.251791398198705 1101 10 0.38179577083264604 1101 11 0.4699479092861907 1101 12 0.5254797199415293 1101 13 0.37296749390139894 1101 14 0.2952040963312754 1101 15 0.25075885955664745 1101 16 0.39557123109177894 1101 17 0.21495042351394034 1101 18 0.5028741346431644 1101 19 0.4291139968577369 1101 20 0.32496247216366214 1101 21 -0.15857181566202172 1101 22 0.0324502090757548 1101 23 0.1839364837802009 1101 24 0.05736926489647942 1101 25 0.06770397152310892 1102 1 0.6400694087411658 1102 2 0.7948449910774009 1102 3 0.668331471269358 1102 4 0.6928213466132505 1102 5 0.8434118635009114 1102 6 0.9228015657152255 1102 7 0.6675439509951104 1102 8 0.8118804769238324 1102 9 1.0420679611940287 1102 10 0.9756553160944819 1102 11 0.9972795595357908 1102 12 1.2790040743668811 1102 13 1.2185751800686617 1102 14 1.1425513297464676 1102 15 1.2844540176990407 1102 16 1.2070685899743694 1102 17 1.2314462355381863 1102 18 1.3392232051913595 1102 19 1.3006947715091144 1102 20 1.293414964515307 1102 21 1.2335987894460487 1102 22 1.0452877965878358 1102 23 1.1251149988725686 1102 24 1.3435928680451923 1102 25 1.2581415223689163 1103 1 0.34839179921307206 1103 2 0.4108423831563002 1103 3 0.3081742613776228 1103 4 0.3877860386673575 1103 5 0.23446595949811666 1103 6 0.5166391583469334 1103 7 0.3659206119378169 1103 8 0.5140336647400616 1103 9 0.6070013398292815 1103 10 0.5643753227061126 1103 11 0.4375811233701654 1103 12 0.5347849355318104 1103 13 0.4940915759951636 1103 14 0.4171773593702497 1103 15 0.5613557339682487 1103 16 0.5332684632514457 1103 17 0.20101142897430435 1103 18 0.6114883315928878 1103 19 0.5486790505199437 1103 20 0.4316843701113129 1103 21 0.6221399147089102 1103 22 0.7692775485598489 1103 23 0.8724094643864859 1103 24 0.7126526390256416 1103 25 0.7699954420559649 1104 1 0.38619591089275573 1104 2 0.38349702001777786 1104 3 0.43346917115408345 1104 4 0.349321185448936 1104 5 0.5511191599649479 1104 6 0.5515507303013294 1104 7 0.5858128823391056 1104 8 0.5559692065301718 1104 9 0.8959159641223025 1104 10 0.7830466576553903 1104 11 0.8319881617039471 1104 12 0.8725280004388907 1104 13 1.0073818174890192 1104 14 1.0411023077498172 1104 15 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-0.07511172815418377 2313 19 0.0513504084380898 2313 20 -0.11322385174947414 2313 21 -0.1500501473616609 2313 22 0.024638852373698228 2313 23 0.07452019562424539 2313 24 0.015362168683424215 2313 25 0.05430055043051141 2314 1 -0.45671522656310515 2314 2 -0.5942357167344241 2314 3 -0.523835123465215 2314 4 -0.3336676477760463 2314 5 -0.45859504198031686 2314 6 -0.4277595046343592 2314 7 -0.4665734421857266 2314 8 -0.3397164156096224 2314 9 -0.5884887932297929 2314 10 -0.4384038120885094 2314 11 -0.489610815949753 2314 12 -0.3749126282658395 2314 13 -0.5267851997327995 2314 14 -0.5947706383156799 2314 15 -0.6200021756544345 2314 16 -0.6423595498616237 2314 17 -0.5952693449538273 2314 18 -0.7097625522536148 2314 19 -0.6034916764839764 2314 20 -0.7860520186525832 2314 21 -0.6421025484909393 2314 22 -0.5788490182589499 2314 23 -0.5470904391938944 2314 24 -0.5263359191796653 2314 25 -0.7162866174670839 2315 1 -0.731561226484633 2315 2 -0.6487389929144349 2315 3 -0.6640217872545975 2315 4 -0.7266302365467894 2315 5 -0.7898570579100053 2315 6 -1.0361934611612185 2315 7 -0.970514176677824 2315 8 -0.7478546331322775 2315 9 -0.9377845126161475 2315 10 -0.9018864208694595 2315 11 -1.063496522388327 2315 12 -1.19988589895285 2315 13 -1.1375083134770634 2315 14 -1.3459305433653128 2315 15 -1.3040114254680617 2315 16 -1.3795638108574266 2315 17 -1.3428073054827294 2315 18 -1.3307764514963623 2315 19 -1.5218262686289918 2315 20 -1.640700957327205 2315 21 -1.3416111214889384 2315 22 -1.3620166625481929 2315 23 -1.382892759984884 2315 24 -1.1466582894076254 2315 25 -1.1515035908260458 2316 1 -1.0501388679789407 2316 2 -1.1124535191289624 2316 3 -0.9905209054203823 2316 4 -1.0315644998143867 2316 5 -1.2381945999975486 2316 6 -1.2434846267857154 2316 7 -1.186799049212695 2316 8 -1.091218830663145 2316 9 -1.5321558049041073 2316 10 -1.54706839938477 2316 11 -1.688070636545774 2316 12 -1.7090198976701885 2316 13 -1.6151969057489879 2316 14 -1.8939353934534964 2316 15 -1.8549523826590217 2316 16 -1.8617016575786067 2316 17 -2.0267899894654136 2316 18 -2.0519064017929836 2316 19 -1.93386330566603 2316 20 -1.7080861184623615 2316 21 -1.7574260164704967 2316 22 -1.8444501241483238 2316 23 -1.7516147016234953 2316 24 -1.7516667069160405 2316 25 -1.8728489892547806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54545.0 3 2.0 4 868.0 5 8473.0 6 10175.0 7 8389.0 8 6734.0 9 6165.0 10 5743.0 11 5500.0 12 5281.0 13 5053.0 14 4869.0 15 4906.0 16 5025.0 17 5342.0 18 5718.0 19 6487.0 20 7670.0 21 10043.0 22 12890.0 23 15890.0 24 19831.0 25 23219.0 26 26662.0 27 30112.0 28 35174.0 29 42936.0 30 53792.0 31 68759.0 32 86415.0 33 111581.0 34 142350.0 35 185543.0 36 263359.0 37 431426.0 38 673012.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.83721563270306 19.56906561010322 14.373161137687626 24.220557619506096 2 15.627553673964064 20.612534462176647 39.628919399198054 24.130992464661233 3 19.5771523875714 24.03835411698792 29.84394162361466 26.54055187182602 4 13.570304514048267 17.261863168892592 36.80721558165292 32.36061673540622 5 13.897417782212429 37.39406779661017 35.1443791895747 13.564135231602704 6 32.93364391308732 36.62348704297474 17.294290774785466 13.148578269152475 7 29.024673851508343 31.392976975562974 20.98133048584085 18.60101868708783 8 25.702078588616693 36.96441624660713 19.665564685960604 17.66794047881557 9 26.156985596703514 16.033714668031276 19.64903706747327 38.16026266779194 10 15.13176696141617 29.63761836599177 33.29532678449115 21.93528788810091 11 35.324123335365464 22.66078757658668 23.09766065158985 18.917428436458 12 23.13757798839217 24.70004464549095 30.953969954881693 21.208407411235182 13 28.96889836937261 21.377323856382947 25.81208139621974 23.84169637802471 14 21.44837169484242 21.553102401358363 27.20366503078112 29.7948608730181 15 23.667507831789848 29.704440155167138 23.29791680875537 23.330135204287643 16 22.954811817372743 28.42110196117976 26.370673059019502 22.253413162427996 17 22.281112614171324 28.195740560742344 27.0890595952449 22.43408722984143 18 22.11976958407725 27.8686192409095 28.398005137369616 21.61360603764364 19 23.601104463335677 27.06617198179535 27.127094038801825 22.205629516067145 20 23.498172965920887 27.27617734176479 27.6287386414465 21.596911050867824 21 23.675792562069574 26.604151821448163 26.97562573772803 22.74442987875423 22 23.283523135946147 27.042865947624605 27.389862251714376 22.28374866471487 23 23.33787598762981 26.791395094184413 27.05684119971263 22.81388771847315 24 23.077241720395374 26.840224792348256 27.598570507448102 22.483962979808272 25 23.378316741778498 26.844968344233965 27.41263803629638 22.364076877691158 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 247.0 2 345.5 3 444.0 4 444.0 5 444.0 6 1100.5 7 1757.0 8 1757.0 9 1757.0 10 2816.0 11 3875.0 12 3875.0 13 3875.0 14 6428.5 15 8982.0 16 8982.0 17 8982.0 18 16493.5 19 24005.0 20 24005.0 21 24005.0 22 38648.0 23 53291.0 24 53291.0 25 53291.0 26 76495.5 27 99700.0 28 99700.0 29 99700.0 30 130021.5 31 160343.0 32 160343.0 33 160343.0 34 184388.5 35 208434.0 36 208434.0 37 208434.0 38 231708.5 39 254983.0 40 254983.0 41 254983.0 42 276245.5 43 297508.0 44 297508.0 45 297508.0 46 307388.0 47 317268.0 48 317268.0 49 317268.0 50 312360.0 51 307452.0 52 307452.0 53 307452.0 54 278698.0 55 249944.0 56 249944.0 57 249944.0 58 215079.0 59 180214.0 60 180214.0 61 180214.0 62 147557.5 63 114901.0 64 114901.0 65 114901.0 66 88654.5 67 62408.0 68 62408.0 69 62408.0 70 45316.0 71 28224.0 72 28224.0 73 28224.0 74 19514.5 75 10805.0 76 10805.0 77 10805.0 78 7272.0 79 3739.0 80 3739.0 81 3739.0 82 2402.0 83 1065.0 84 1065.0 85 1065.0 86 670.5 87 276.0 88 276.0 89 276.0 90 169.0 91 62.0 92 62.0 93 62.0 94 36.0 95 10.0 96 10.0 97 10.0 98 6.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3929388992773456 2 0.0 3 0.0 4 0.0 5 0.028243398680886836 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007071310188251667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2389939.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.5202355953369 #Duplication Level Percentage of deduplicated Percentage of total 1 86.31687929450365 65.18671060175173 2 9.348878281314343 14.12058980713975 3 2.1736603887043286 4.924660339776075 4 0.8023860224616103 2.4238552581882433 5 0.4128746614895085 1.5590195853516324 6 0.24768705484654358 1.122323084155566 7 0.14667127221667692 0.7753654323009862 8 0.10476714309548953 0.6329631463377393 9 0.07644263568948867 0.5195669271118825 >10 0.3410311914305355 4.377616248183988 >50 0.01622536067762969 0.8502535461731124 >100 0.010390263972570596 1.5002225778748235 >500 0.0011640795145149273 0.630953819083766 >1k 8.869177253358104E-4 1.154948574480901 >5k 5.543235783348815E-5 0.22095105208997048 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5278 0.2208424566484751 No Hit GGTATCAACGCAGAGTACTTTTTTT 3517 0.14715856764545035 No Hit TATCAACGCAGAGTACTTTTTTTTT 3385 0.1416354141256325 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.184207211983235E-5 0.0 5 0.0 0.0 0.0 4.184207211983235E-5 0.0 6 0.0 0.0 0.0 4.184207211983235E-5 0.0 7 0.0 0.0 0.0 4.184207211983235E-5 0.0 8 0.0 0.0 0.0 4.184207211983235E-5 0.0 9 0.0 0.0 0.0 4.184207211983235E-5 4.184207211983235E-5 10 0.0 0.0 0.0 4.184207211983235E-5 4.184207211983235E-5 11 0.0 0.0 0.0 4.184207211983235E-5 4.184207211983235E-5 12 0.0 0.0 0.0 4.184207211983235E-5 4.184207211983235E-5 13 0.0 0.0 0.0 8.36841442396647E-5 4.184207211983235E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGTG 65 3.3833403E-6 14.611474 7 TAGGACC 405 0.0 11.962515 4 AACACCG 75 2.0754256E-4 11.399573 5 TCCAACG 300 0.0 11.39695 18 ATAGGAC 270 0.0 11.258838 3 TAGGACT 315 0.0 11.158313 4 GATATAC 740 0.0 10.825289 1 ACACCGT 80 3.7816117E-4 10.684641 6 GTCCTAC 1065 0.0 10.4767885 1 GGTATCA 1585 0.0 10.469161 1 CACTGTT 955 0.0 10.342782 7 AGGACGT 940 0.0 10.308125 5 ATACACT 1035 0.0 10.096241 4 GAACAGT 645 0.0 10.012824 6 ATTAGAC 200 2.5465852E-11 9.974627 3 GTAGGAC 990 0.0 9.883469 3 ACTGTTC 985 0.0 9.834931 8 CCAACGT 145 1.6228114E-7 9.825574 19 ACAGCGC 145 1.624303E-7 9.824957 8 TGTAGAA 970 0.0 9.695513 2 >>END_MODULE