FastQCFastQC Report
Thu 26 May 2016
SRR1512243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146740
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47620.22182472027353103No Hit
GTCCTACAGTGGACATTTCTAAATT39870.18572346907403783No Hit
CTGTAGGACGTGGAATATGGCAAGA33680.15688905037405554No Hit
GTCCTAAAGTGTGTATTTCTCATTT32280.15036753402834063No Hit
GGTATCAACGCAGAGTACTTTTTTT29820.13890829816372732No Hit
CTTTAGGACGTGAAATATGGCGAGG29140.13574070451009437No Hit
TATCAACGCAGAGTACTTTTTTTTT28660.1335047560487064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7750.012.2827464
TCCAACG1700.012.1980418
ACGAAAT1657.2759576E-1211.42436212
CGAAATC1605.2750693E-1111.19230613
TGTAGGA22000.010.9756152
GGTATCA12800.010.8251051
AGGACCT15100.010.6732075
GTAGGAC22350.010.626713
CTGTAGG21900.010.5605111
GTATCAA25000.010.3105941
GGACCTG14500.010.1398716
CCAACGA1401.0869917E-710.09902719
GGACGTG21050.09.9397786
GTACCGT1054.4802786E-59.8736846
TGGCGAG22250.09.82820618
GGCGAGG9350.09.77859319
AGGACGT21400.09.7772115
GTCTAGA2007.4942363E-109.6789171
GTCCTAA11500.09.6567691
GTCCACG850.00418181959.5890211
Produced by FastQC (version 0.11.5)