FastQCFastQC Report
Thu 26 May 2016
SRR1512243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146740
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64850.3020859535854365No Hit
TATCAACGCAGAGTACTTTTTTTTT42320.1971361226790389No Hit
GGTATCAACGCAGAGTACTTTTTTT41200.191918909602467No Hit
GTCCTACAGTGGACATTTCTAAATT40610.1891705562853443No Hit
CTGTAGGACGTGGAATATGGCAAGA36200.16862777979634236No Hit
CTTTAGGACGTGAAATATGGCGAGG33970.15823993590281077No Hit
GTCCTAAAGTGTGTATTTCTCATTT32540.15157867277825912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6450.013.40436654
TCCAACG1457.2759576E-1212.44452218
GGTATCA16400.012.4433221
TACACCG550.003066931912.0920515
TAGAAAT7500.010.7676834
GGACGTG20600.010.64976
TGTAGGA22850.010.3948552
AGGACGT21600.010.380615
AGGACCT14400.010.3586175
GACGTGG10100.010.3434147
CCACCTT6900.010.32297313
TTTAGAA8400.010.2926392
CCAACGA951.648335E-49.99768419
GTAGGAC22000.09.9759423
CACCTTT7150.09.9620314
CGTACAC1054.101931E-59.95332053
CTGTAGG22850.09.9324681
TGGCGAG20100.09.87513118
GTCCTAC19850.09.8002251
ATTTCTA14450.09.79289315
Produced by FastQC (version 0.11.5)