##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512243_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2146740 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.176517882929467 31.0 30.0 34.0 26.0 34.0 2 30.388360956613283 31.0 30.0 34.0 27.0 34.0 3 30.455204635866476 31.0 30.0 34.0 27.0 34.0 4 34.08036697504122 37.0 35.0 37.0 30.0 37.0 5 33.5943649440547 35.0 35.0 37.0 28.0 37.0 6 33.51484017626727 36.0 35.0 37.0 28.0 37.0 7 33.305136625767446 36.0 35.0 37.0 27.0 37.0 8 33.18699423311626 35.0 33.0 37.0 27.0 37.0 9 34.57760557869141 38.0 35.0 39.0 27.0 39.0 10 34.337711599914286 38.0 34.0 39.0 27.0 39.0 11 34.32423581803106 38.0 34.0 39.0 27.0 39.0 12 34.10694681237597 37.0 34.0 39.0 26.0 39.0 13 34.07434621798634 37.0 34.0 39.0 26.0 39.0 14 34.943882351845126 38.0 34.0 40.0 25.0 41.0 15 34.84031368493623 38.0 34.0 40.0 25.0 41.0 16 34.71742269674017 38.0 34.0 40.0 25.0 41.0 17 34.62973625124607 38.0 34.0 40.0 25.0 41.0 18 34.54617140408247 38.0 33.0 40.0 24.0 41.0 19 34.55586843306595 38.0 34.0 40.0 24.0 41.0 20 34.408934011571034 38.0 34.0 40.0 23.0 41.0 21 34.274431929344026 38.0 34.0 40.0 22.0 41.0 22 34.35291232287096 38.0 34.0 40.0 22.0 41.0 23 34.21517091031052 38.0 34.0 40.0 19.0 41.0 24 34.043842756924455 38.0 34.0 40.0 18.0 41.0 25 33.54032393303334 38.0 33.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33193200069931805 1101 2 0.48374075852640175 1101 3 0.5416138896091915 1101 4 0.5439666815756397 1101 5 0.5901835894638268 1101 6 0.6411155802821753 1101 7 0.5802910884522916 1101 8 0.2988268323442682 1101 9 0.5163136651694629 1101 10 0.7232978108448265 1101 11 0.6087695270855633 1101 12 0.799212647696919 1101 13 0.6461512922472323 1101 14 0.7029766404857014 1101 15 0.6755238827991832 1101 16 0.6494644222353614 1101 17 0.5959579434913351 1101 18 0.6866379352353817 1101 19 0.7918501467687236 1101 20 0.6757206852502691 1101 21 0.6165646498988551 1101 22 0.5674955958368741 1101 23 0.5999354764382829 1101 24 0.5498824945327527 1101 25 0.4124199148378267 1102 1 0.4273690171884752 1102 2 0.4693484768940728 1102 3 0.42881921449567884 1102 4 0.5015088650415009 1102 5 0.4984016923910133 1102 6 0.6787482455837264 1102 7 0.47852338920248627 1102 8 0.6627491332350317 1102 9 0.886053324890284 1102 10 0.6516498793692378 1102 11 0.7118122189553233 1102 12 0.8798717453151497 1102 13 0.9220499019851545 1102 14 0.8556025348782654 1102 15 1.0685800696294194 1102 16 0.8773725159316186 1102 17 0.9343675061702754 1102 18 1.0951650633859629 1102 19 0.937118007676915 1102 20 1.0541751374157897 1102 21 0.9903525634748789 1102 22 1.0399213618398804 1102 23 0.9331213648330845 1102 24 0.9473854582697356 1102 25 1.034938224406595 1103 1 0.5268903432350456 1103 2 0.6265532599948678 1103 3 0.6141107999551885 1103 4 0.7167901789229987 1103 5 0.6083852933551412 1103 6 0.6224070791520404 1103 7 0.6934619427944924 1103 8 0.698509408575319 1103 9 1.0175084097032396 1103 10 0.947414498735732 1103 11 0.8994617975465928 1103 12 1.044624217805044 1103 13 1.064514644956489 1103 14 0.8670297496409418 1103 15 1.047070401475736 1103 16 1.2389208310940916 1103 17 0.9930172507509667 1103 18 1.183496591200651 1103 19 1.1735666786497276 1103 20 0.9454904766575112 1103 21 1.2574636804758583 1103 22 1.1830448963767992 1103 23 1.43808835674767 1103 24 1.0470545800374609 1103 25 1.0418893401856764 1104 1 0.5090855240141821 1104 2 0.5339019104729772 1104 3 0.5324096787899855 1104 4 0.5543464900482888 1104 5 0.6175133364900489 1104 6 0.6568620963857725 1104 7 0.6982531539796923 1104 8 0.5350651517265632 1104 9 0.8650174933977155 1104 10 0.7726589998911351 1104 11 0.9266202934268364 1104 12 0.9840921895152661 1104 13 0.8435322832737313 1104 14 1.115735469061768 1104 15 1.1684285596837327 1104 16 1.1636168093710282 1104 17 1.371319086162984 1104 18 1.1578879642195474 1104 19 1.3260062438772664 1104 20 1.280870591341582 1104 21 1.1952183934933558 1104 22 1.3076537334304916 1104 23 1.34457804373951 1104 24 1.4630714480885345 1104 25 1.4427280744594029 1105 1 0.11733075042830876 1105 2 0.28282112276720284 1105 3 0.3516187678342426 1105 4 0.2992414882533865 1105 5 0.18285270126551723 1105 6 0.205676178677777 1105 7 0.32879961631238075 1105 8 0.20225604387856322 1105 9 0.26644465454577215 1105 10 0.3399172008860205 1105 11 0.49330284355589527 1105 12 0.33253724840516696 1105 13 0.22666639667738053 1105 14 0.36981374390407495 1105 15 0.3077337187462845 1105 16 0.3601278608419918 1105 17 -0.011376558207011556 1105 18 0.3081328402003365 1105 19 0.36499280775014853 1105 20 0.3500151493682182 1105 21 0.38828088174101794 1105 22 0.6130401502923206 1105 23 0.5879061348179917 1105 24 0.66087205151765 1105 25 0.5217057065556787 1106 1 0.013062421433701843 1106 2 0.0693970164598845 1106 3 -0.04616911946094504 1106 4 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0.4129271331464892 1107 16 0.48396022148120466 1107 17 0.6013384369972599 1107 18 0.5755572057815925 1107 19 0.4946495469760066 1107 20 0.5009318255179736 1107 21 0.43237095213264354 1107 22 0.4121492816954415 1107 23 0.3766670471454674 1107 24 0.19947344471908934 1107 25 0.12437607704647036 1108 1 -0.05652536685399667 1108 2 -0.03552341187995367 1108 3 -0.03952794229438794 1108 4 0.0835437514820967 1108 5 0.06505244322364234 1108 6 0.11704903293286861 1108 7 0.09647784069831289 1108 8 0.08148334821847669 1108 9 0.14118913134475264 1108 10 0.21536203062388637 1108 11 0.10968777118068118 1108 12 0.05664897013593162 1108 13 0.2231008527420144 1108 14 0.18349955030117115 1108 15 0.21555809102855505 1108 16 0.3102126831700929 1108 17 0.42045562032937056 1108 18 0.25936568886559996 1108 19 0.2995959302517903 1108 20 0.36159656580359467 1108 21 0.464987809222805 1108 22 0.4977240327500212 1108 23 0.4369500149357677 1108 24 0.6031900247514983 1108 25 0.33020995742177917 1109 1 0.25277829032171084 1109 2 0.21126837331153325 1109 3 0.15187418633531635 1109 4 0.2908493659018916 1109 5 0.2290904039297672 1109 6 0.4135155403833082 1109 7 0.29142742547627165 1109 8 0.272694159944308 1109 9 0.44614262046972186 1109 10 0.5605468708362409 1109 11 0.5494379631801607 1109 12 0.3285001493774402 1109 13 0.5940132335004904 1109 14 0.4215892441902227 1109 15 0.3358123689408359 1109 16 0.5733141856611113 1109 17 0.5606084164229941 1109 18 0.6573296167557032 1109 19 0.6674764938975244 1109 20 0.6108811263929397 1109 21 0.6574184856109468 1109 22 0.5937672637395366 1109 23 0.7082617624177345 1109 24 0.5342972172139611 1109 25 0.5985372782167389 1110 1 -0.19273508343501078 1110 2 -0.154277554583107 1110 3 -0.21444975523384002 1110 4 -0.2237206643371934 1110 5 -0.42381902043891984 1110 6 -0.3866207669925359 1110 7 -0.3787358469701303 1110 8 -0.17886528671283486 1110 9 -0.35074660344032793 1110 10 -0.4130748622473348 1110 11 -0.5373322591213636 1110 12 -0.5293684147436934 1110 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-0.11075158917579131 2313 20 -0.23813197754905246 2313 21 -0.0683178031041507 2313 22 -0.09532989376054246 2313 23 -0.10840403592705172 2313 24 0.1310531666660495 2313 25 0.12639034271761318 2314 1 -0.3787129405598897 2314 2 -0.5774085528103825 2314 3 -0.6028238468473788 2314 4 -0.5349974215098925 2314 5 -0.7114642454095232 2314 6 -0.5630697804875382 2314 7 -0.6964958136011958 2314 8 -0.6028125292812376 2314 9 -0.8491195084431524 2314 10 -0.719583601815053 2314 11 -0.7940069443998397 2314 12 -0.9369799831194356 2314 13 -1.153459997508186 2314 14 -1.026959132295687 2314 15 -0.9019038086220732 2314 16 -0.8338837392816885 2314 17 -0.8818357922365365 2314 18 -0.9598666382001326 2314 19 -0.8910239365317736 2314 20 -0.9642472011404237 2314 21 -1.1163297691964544 2314 22 -0.8118635724291181 2314 23 -0.9954381302420217 2314 24 -0.8215944349316899 2314 25 -1.0031950099409173 2315 1 -0.588412474977904 2315 2 -0.5059240759498138 2315 3 -0.5197588208987476 2315 4 -0.6220727433433453 2315 5 -0.8018401156191572 2315 6 -0.7644123233219275 2315 7 -0.888314895111705 2315 8 -0.7975690640265611 2315 9 -0.9186943021733853 2315 10 -0.9075295395213132 2315 11 -0.8118005407711308 2315 12 -0.9732937530335732 2315 13 -1.0019365454696327 2315 14 -1.19040688412354 2315 15 -1.1943971599379992 2315 16 -1.4686527782731176 2315 17 -1.282173043205887 2315 18 -1.4002642902493676 2315 19 -1.3905294926418676 2315 20 -1.227707062587804 2315 21 -1.029426612880222 2315 22 -1.1256578605575882 2315 23 -1.171277350088495 2315 24 -0.9486574227501094 2315 25 -1.0804139526357233 2316 1 -0.9269810906098108 2316 2 -0.9299534108905405 2316 3 -1.0007425460343704 2316 4 -1.1078903041229324 2316 5 -1.150839512846403 2316 6 -1.1338899202678832 2316 7 -1.239843124969049 2316 8 -1.1652897793168933 2316 9 -1.5645906252595836 2316 10 -1.5674700659428495 2316 11 -1.576909240791224 2316 12 -1.7401492884967027 2316 13 -1.8245967825602492 2316 14 -2.184365493727718 2316 15 -1.9046081304021314 2316 16 -1.841455269733835 2316 17 -2.0276900213051405 2316 18 -1.7572564542035565 2316 19 -1.7477736156075139 2316 20 -1.862950381856443 2316 21 -1.760390245590692 2316 22 -1.7114042941521248 2316 23 -1.525120629172278 2316 24 -1.5455322469413915 2316 25 -1.6033952666803266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48657.0 3 2.0 4 804.0 5 7631.0 6 9129.0 7 7598.0 8 6067.0 9 5453.0 10 5242.0 11 4962.0 12 4752.0 13 4345.0 14 4438.0 15 4399.0 16 4593.0 17 4751.0 18 5102.0 19 5928.0 20 7042.0 21 9081.0 22 11843.0 23 14837.0 24 17649.0 25 20628.0 26 23844.0 27 27797.0 28 32065.0 29 39177.0 30 49049.0 31 62197.0 32 78746.0 33 100835.0 34 128491.0 35 168469.0 36 239796.0 37 393900.0 38 587441.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.66378340359105 20.451635350775703 14.699450689747737 24.185130555885515 2 15.11119185369444 21.157196493287497 40.40139933107875 23.33021232193931 3 18.94463232622488 24.99958075966349 30.516830170397906 25.538956743713726 4 13.472567707314345 17.905056038458316 37.47920102108313 31.14317523314421 5 13.43816589153572 37.87424473076373 35.50952453509616 13.178064842604384 6 31.91136327640981 37.37904916291679 17.692687516886068 13.016900043787324 7 28.122548608587906 31.86016937309595 21.975553630155492 18.041728388160653 8 24.97368102331908 37.60450729944008 20.224246997773367 17.197564679467472 9 26.14559751064405 15.831493334078651 20.501458024725864 37.52145113055144 10 15.461071205642044 29.352273680091674 33.78639239032207 21.400262723944213 11 34.06858771905308 22.68583992472307 24.61122446127617 18.634347894947688 12 23.545468943607517 24.81506842933937 31.358198943514353 20.28126368353876 13 28.850536161808137 22.054743471496316 25.993879091086953 23.100841275608598 14 21.014887690172074 22.642611587802904 27.44910888137362 28.893391840651404 15 22.885864147498065 31.360854132312248 23.430503926884487 22.3227777933052 16 22.10477281831987 28.4929241547649 27.75319787212238 21.64910515479285 17 21.594557328786905 29.003093062038253 27.81887885817565 21.58347075099919 18 21.427187270000093 27.62812450506349 30.0927918611476 20.851896363788814 19 23.492504914428388 27.002524758470987 27.829313284328794 21.67565704277183 20 23.58678740788358 27.36009018325461 28.234578942955363 20.818543465906444 21 22.953082348118542 26.861986081220827 27.4410501504607 22.743881420199934 22 23.268351081174245 27.940551720282848 27.645779181456536 21.145318017086375 23 22.381424858157022 27.7513811639975 27.989323346096874 21.877870631748607 24 23.124039240895495 26.880386073767664 28.569272478269376 21.42630220706746 25 22.75653687333224 27.38876321163966 28.33020900105842 21.52449091396968 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 364.0 1 364.0 2 481.5 3 599.0 4 599.0 5 599.0 6 1491.0 7 2383.0 8 2383.0 9 2383.0 10 3376.0 11 4369.0 12 4369.0 13 4369.0 14 7081.5 15 9794.0 16 9794.0 17 9794.0 18 17697.0 19 25600.0 20 25600.0 21 25600.0 22 40880.5 23 56161.0 24 56161.0 25 56161.0 26 81537.0 27 106913.0 28 106913.0 29 106913.0 30 137408.5 31 167904.0 32 167904.0 33 167904.0 34 189081.0 35 210258.0 36 210258.0 37 210258.0 38 225518.0 39 240778.0 40 240778.0 41 240778.0 42 254411.0 43 268044.0 44 268044.0 45 268044.0 46 272312.0 47 276580.0 48 276580.0 49 276580.0 50 266902.0 51 257224.0 52 257224.0 53 257224.0 54 229616.5 55 202009.0 56 202009.0 57 202009.0 58 172850.5 59 143692.0 60 143692.0 61 143692.0 62 117015.5 63 90339.0 64 90339.0 65 90339.0 66 69373.5 67 48408.0 68 48408.0 69 48408.0 70 35324.0 71 22240.0 72 22240.0 73 22240.0 74 15536.0 75 8832.0 76 8832.0 77 8832.0 78 5893.5 79 2955.0 80 2955.0 81 2955.0 82 1951.0 83 947.0 84 947.0 85 947.0 86 610.0 87 273.0 88 273.0 89 273.0 90 168.0 91 63.0 92 63.0 93 63.0 94 36.0 95 9.0 96 9.0 97 9.0 98 5.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3923157904543634 2 0.0 3 0.0 4 0.0 5 0.030325051007574273 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006894174422612891 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2146740.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.34321791147998 #Duplication Level Percentage of deduplicated Percentage of total 1 85.03485942547927 62.367302249149944 2 10.435614675210724 15.307631223284371 3 2.3932799127261863 5.265925504867336 4 0.8356620438431238 2.4516057352775587 5 0.39847953273555986 1.4612885601344443 6 0.23536520926385157 1.0357465099091852 7 0.1473775066204549 0.7566398408320226 8 0.10430817429330147 0.6120237725713796 9 0.06710339930189077 0.44294213138396654 >10 0.3120419447750147 3.9017991916420414 >50 0.018818231814391254 0.9692876154747957 >100 0.01347092599328286 1.9767465170952399 >500 0.0022222040000394174 1.1171826745478501 >1k 0.0013333224000233138 2.0318931608191226 >5k 6.349154285825303E-5 0.3019853130107717 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6485 0.3020859535854365 No Hit TATCAACGCAGAGTACTTTTTTTTT 4232 0.1971361226790389 No Hit GGTATCAACGCAGAGTACTTTTTTT 4120 0.191918909602467 No Hit GTCCTACAGTGGACATTTCTAAATT 4061 0.1891705562853443 No Hit CTGTAGGACGTGGAATATGGCAAGA 3620 0.16862777979634236 No Hit CTTTAGGACGTGAAATATGGCGAGG 3397 0.15823993590281077 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3254 0.15157867277825912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.658225961224927E-5 0.0 5 0.0 9.316451922449854E-5 0.0 4.658225961224927E-5 0.0 6 0.0 9.316451922449854E-5 0.0 4.658225961224927E-5 0.0 7 0.0 9.316451922449854E-5 0.0 4.658225961224927E-5 0.0 8 0.0 9.316451922449854E-5 0.0 4.658225961224927E-5 0.0 9 0.0 9.316451922449854E-5 0.0 4.658225961224927E-5 4.658225961224927E-5 10 0.0 9.316451922449854E-5 0.0 4.658225961224927E-5 4.658225961224927E-5 11 0.0 9.316451922449854E-5 0.0 4.658225961224927E-5 4.658225961224927E-5 12 0.0 9.316451922449854E-5 0.0 4.658225961224927E-5 4.658225961224927E-5 13 0.0 9.316451922449854E-5 0.0 4.658225961224927E-5 4.658225961224927E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 645 0.0 13.4043665 4 TCCAACG 145 7.2759576E-12 12.444522 18 GGTATCA 1640 0.0 12.443322 1 TACACCG 55 0.0030669319 12.092051 5 TAGAAAT 750 0.0 10.767683 4 GGACGTG 2060 0.0 10.6497 6 TGTAGGA 2285 0.0 10.394855 2 AGGACGT 2160 0.0 10.38061 5 AGGACCT 1440 0.0 10.358617 5 GACGTGG 1010 0.0 10.343414 7 CCACCTT 690 0.0 10.322973 13 TTTAGAA 840 0.0 10.292639 2 CCAACGA 95 1.648335E-4 9.997684 19 GTAGGAC 2200 0.0 9.975942 3 CACCTTT 715 0.0 9.96203 14 CGTACAC 105 4.101931E-5 9.9533205 3 CTGTAGG 2285 0.0 9.932468 1 TGGCGAG 2010 0.0 9.875131 18 GTCCTAC 1985 0.0 9.800225 1 ATTTCTA 1445 0.0 9.792893 15 >>END_MODULE