FastQCFastQC Report
Thu 26 May 2016
SRR1512242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469876
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51270.20758127128649376No Hit
CTGTAGGACGTGGAATATGGCAAGA44910.18183099070560627No Hit
GTATCAACGCAGAGTACTTTTTTTT43890.1777012287256526No Hit
GTCCTAAAGTGTGTATTTCTCATTT38300.15506851356100468No Hit
CTTTAGGACGTGAAATATGGCGAGG34330.1389948321292243No Hit
GGTATCAACGCAGAGTACTTTTTTT27300.11053186475758296No Hit
TATCAACGCAGAGTACTTTTTTTTT24780.10032892339534455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2850.013.2307918
CGTTAGG550.00183225912.9511151
GCCGATC604.3734052E-412.5677238
GGCGAGG11300.012.43020919
TAGGACC8450.012.270514
ACCGTAT701.1742648E-412.11887558
CCAACGA1900.011.90771119
TGTAGGA25400.011.6581892
CACCGTA600.006229524510.996987
GTAGGAC26250.010.9880033
GGACGTG26500.010.95548256
AGGACGT27100.010.9216185
TGGCGAG25650.010.87864818
CTGTAGG25900.010.8045011
GACGGTG700.001592499210.7725527
GACGTGA14400.010.6696897
CATACGT804.051215E-410.60530519
GTGTAGG5050.010.4781291
GACGTGG13500.010.4733147
GGTATCA13650.010.4367961
Produced by FastQC (version 0.11.5)