##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512242_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2469876 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.172064103623015 31.0 30.0 34.0 24.0 34.0 2 26.320422563723845 31.0 26.0 34.0 2.0 34.0 3 27.966957855374115 31.0 27.0 34.0 16.0 34.0 4 32.47904712625249 35.0 32.0 37.0 25.0 37.0 5 32.87597838919849 35.0 33.0 37.0 26.0 37.0 6 33.082500093122086 35.0 35.0 37.0 27.0 37.0 7 33.07488958959883 36.0 35.0 37.0 26.0 37.0 8 33.08154538932319 37.0 35.0 37.0 26.0 37.0 9 34.56707219309795 39.0 35.0 39.0 27.0 39.0 10 34.421131668148526 38.0 35.0 39.0 27.0 39.0 11 34.442074015051766 38.0 35.0 39.0 27.0 39.0 12 34.31448056501622 38.0 35.0 39.0 27.0 39.0 13 34.310800218310554 38.0 35.0 39.0 27.0 39.0 14 35.33841779911218 39.0 35.0 41.0 26.0 41.0 15 35.26539834388447 39.0 35.0 41.0 26.0 41.0 16 35.21429739792605 39.0 35.0 41.0 26.0 41.0 17 35.17937337744891 39.0 35.0 41.0 25.0 41.0 18 35.12077650861825 39.0 35.0 41.0 25.0 41.0 19 35.14142531851802 39.0 35.0 41.0 25.0 41.0 20 35.050751130826 39.0 35.0 41.0 25.0 41.0 21 34.96746840732085 39.0 35.0 41.0 25.0 41.0 22 34.85422588016564 39.0 35.0 41.0 24.0 41.0 23 34.72832158375562 39.0 34.0 41.0 24.0 41.0 24 34.59765227080226 39.0 34.0 41.0 22.0 41.0 25 34.459134790572485 39.0 34.0 41.0 20.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4153672786784455 1101 2 3.3353131891675005 1101 3 1.8886706541072016 1101 4 0.813314519968344 1101 5 0.32835567156970313 1101 6 0.4665683174638886 1101 7 0.09981654659975447 1101 8 0.09763991593310806 1101 9 0.17293362007079338 1101 10 0.140591834649328 1101 11 0.21448296621599638 1101 12 0.10086276388355486 1101 13 0.01245885876370778 1101 14 -0.023454539621688753 1101 15 0.1839681312976964 1101 16 0.31452229464950676 1101 17 0.0978111251189091 1101 18 0.24190098856605147 1101 19 0.05464155388366976 1101 20 0.14203047430115845 1101 21 0.10663521653463448 1101 22 0.2524204883555612 1101 23 0.3374659393891264 1101 24 0.20978863833839512 1101 25 0.17176069936311933 1102 1 1.071380037948515 1102 2 3.984810388597065 1102 3 2.2758657063750505 1102 4 1.3797483309951204 1102 5 0.8471636820253536 1102 6 0.9096901067814827 1102 7 0.9374032008271342 1102 8 0.8176786259322597 1102 9 0.7884920407249894 1102 10 0.7479918234290395 1102 11 0.7208651985410981 1102 12 0.7980001355514972 1102 13 0.7665144393686703 1102 14 0.8991514349747476 1102 15 1.0139871503811904 1102 16 0.8448203664912199 1102 17 0.8846722006182972 1102 18 1.1770016560347614 1102 19 0.9665595100178805 1102 20 0.7899014243480096 1102 21 1.0895149246408806 1102 22 1.0029612439797333 1102 23 1.0733832638625884 1102 24 1.1219039884564523 1102 25 1.0389050248401688 1103 1 0.8843851397969757 1103 2 3.829843256404118 1103 3 2.3023031848312208 1103 4 1.2247394576297594 1103 5 0.9386803609899559 1103 6 0.6845592616708558 1103 7 0.6193724954821107 1103 8 0.7384924239480739 1103 9 0.7126000251380233 1103 10 0.7502047788117636 1103 11 0.6465867822281055 1103 12 0.7411767666887954 1103 13 0.6881632291439672 1103 14 0.7762883137045264 1103 15 0.9298827274327053 1103 16 0.9390498850404612 1103 17 0.9522025168278745 1103 18 0.7106440311344002 1103 19 0.8439614788547303 1103 20 0.9387280597940801 1103 21 0.9181249974590315 1103 22 0.936286621804669 1103 23 0.9406655230564169 1103 24 0.9945688391577008 1103 25 0.97675780507155 1104 1 1.1886385295514472 1104 2 3.929966034393015 1104 3 2.395006669060745 1104 4 1.4589548000622514 1104 5 1.1151347952479824 1104 6 0.8875521483324533 1104 7 0.8064377254945683 1104 8 0.7164512679217268 1104 9 0.7888373382990892 1104 10 0.8519521715113214 1104 11 0.7402378209259339 1104 12 0.7361440896668157 1104 13 0.8296551301510036 1104 14 1.0489583688061757 1104 15 0.6867836289041875 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-1.4266746653765452 2315 22 -1.2367298202624255 2315 23 -1.2613170408775929 2315 24 -1.2558161697485275 2315 25 -1.1479479712548368 2316 1 -1.273717075316494 2316 2 1.79144863800218E-4 2316 3 -0.41940809595101314 2316 4 -1.1246715555683764 2316 5 -1.2777446249266724 2316 6 -1.2399953516399478 2316 7 -1.2440338702027454 2316 8 -1.2885388512008085 2316 9 -1.3803464681019548 2316 10 -1.3592180040628534 2316 11 -2.079989549656652 2316 12 -1.5083441471209582 2316 13 -1.5461011157779652 2316 14 -1.750419798318653 2316 15 -2.0771388402479687 2316 16 -1.6388726664644437 2316 17 -1.6303901819488829 2316 18 -2.074929061574437 2316 19 -1.9875548477990606 2316 20 -1.9545287706421064 2316 21 -1.9496874522432108 2316 22 -1.764191458430325 2316 23 -1.7439599598021047 2316 24 -1.6326316879628493 2316 25 -1.549065066327131 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99358.0 3 4.0 4 936.0 5 4328.0 6 5191.0 7 4761.0 8 4277.0 9 4212.0 10 4160.0 11 3918.0 12 4010.0 13 3813.0 14 3910.0 15 4079.0 16 4303.0 17 4735.0 18 5461.0 19 6623.0 20 8495.0 21 10983.0 22 13836.0 23 16281.0 24 19293.0 25 22129.0 26 25024.0 27 28902.0 28 32944.0 29 40441.0 30 50533.0 31 64675.0 32 83418.0 33 109421.0 34 147888.0 35 204552.0 36 286796.0 37 366482.0 38 769704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81498342426907 18.795801894508067 13.2189227313436 25.170291949879264 2 15.876643641265295 20.27244976633676 40.662692730651756 23.188213861746192 3 19.205579551362092 23.614100464962615 29.30495296120129 27.875367022474002 4 12.475929965714878 16.32648764553362 37.89109250828787 33.30648988046364 5 13.405693241280128 37.50325927293516 35.60915608718818 13.48189139859653 6 32.35197303826799 36.64078002454379 17.854738162438444 13.152508774749775 7 28.636636767959473 31.270751743551372 21.621332711388394 18.471278777100764 8 24.982995097729603 37.61593699440782 20.15327895003636 17.247788957826224 9 26.148073830427116 15.885574822379747 20.301059648338622 37.66529169885452 10 15.238621989793359 29.05990207971288 33.9357525658322 21.765723364661564 11 34.39638320649507 22.454421632091837 24.54603832194515 18.603156839467943 12 23.653250046965997 24.264577273640093 31.485558765798388 20.596613913595522 13 28.86476891957329 21.921221956082007 25.774168419791117 23.43984070455359 14 20.780665201541133 22.306576707743087 27.322755710021752 29.59000238069403 15 22.769199749299155 31.271407957322555 23.20456573528388 22.754826558094415 16 22.12200126646034 28.171940615642242 27.87152067553189 21.83453744236553 17 21.62452143268963 28.734727953513378 27.923714265354644 21.71703634844235 18 21.399613375866846 27.52793792633733 30.080677620747153 20.991771077048664 19 23.4724884959622 26.90582378628099 27.465710578046266 22.155977139710544 20 23.411944567257628 27.378621436865657 28.240486566936962 20.968947428939753 21 22.700167995228597 26.885634079749348 27.20101485476248 23.21318307025958 22 22.997005067941675 28.118917872784422 27.41138187549877 21.472695183775137 23 22.22892539463578 27.74635609474255 27.857174380690974 22.167544129930693 24 23.035954058882506 26.721050730564837 28.553172259754227 21.68982295079843 25 22.669471880444767 27.330143455425727 28.17988676297847 21.820497901151036 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 308.0 1 308.0 2 367.5 3 427.0 4 427.0 5 427.0 6 1139.0 7 1851.0 8 1851.0 9 1851.0 10 3027.5 11 4204.0 12 4204.0 13 4204.0 14 7126.0 15 10048.0 16 10048.0 17 10048.0 18 18515.5 19 26983.0 20 26983.0 21 26983.0 22 43356.5 23 59730.0 24 59730.0 25 59730.0 26 86649.0 27 113568.0 28 113568.0 29 113568.0 30 149758.5 31 185949.0 32 185949.0 33 185949.0 34 210564.0 35 235179.0 36 235179.0 37 235179.0 38 255320.0 39 275461.0 40 275461.0 41 275461.0 42 293108.0 43 310755.0 44 310755.0 45 310755.0 46 317355.0 47 323955.0 48 323955.0 49 323955.0 50 312536.5 51 301118.0 52 301118.0 53 301118.0 54 269869.5 55 238621.0 56 238621.0 57 238621.0 58 204917.5 59 171214.0 60 171214.0 61 171214.0 62 139734.5 63 108255.0 64 108255.0 65 108255.0 66 83355.5 67 58456.0 68 58456.0 69 58456.0 70 42966.0 71 27476.0 72 27476.0 73 27476.0 74 19143.0 75 10810.0 76 10810.0 77 10810.0 78 7319.5 79 3829.0 80 3829.0 81 3829.0 82 2521.0 83 1213.0 84 1213.0 85 1213.0 86 788.5 87 364.0 88 364.0 89 364.0 90 219.5 91 75.0 92 75.0 93 75.0 94 49.5 95 24.0 96 24.0 97 24.0 98 13.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.367495372237311 3 0.0 4 0.0 5 0.0 6 2.0243931274282597E-4 7 0.008259523959907299 8 0.0 9 0.0 10 0.0045346406054393015 11 0.0025912232031081723 12 1.6195145019426077E-4 13 0.0 14 3.2390290038852154E-4 15 0.0 16 0.0 17 1.6195145019426077E-4 18 0.011903431589278165 19 0.0036843954919194323 20 0.0 21 0.006599521595416126 22 0.001498050914296912 23 0.002712686790753868 24 0.011336601513598254 25 0.007652206021678822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2469876.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.84833676353527 #Duplication Level Percentage of deduplicated Percentage of total 1 85.0208958240184 62.78651746749561 2 10.364370668051704 15.307830708727785 3 2.4506288756969705 5.4292459948474106 4 0.8711005568226053 2.5731730910055544 5 0.4137100408964611 1.5275899211288904 6 0.22914133229656858 1.015302376432928 7 0.13113871247482164 0.6779063061103938 8 0.095713179373464 0.5654607282464182 9 0.06902502556090559 0.4587644999460047 >10 0.3206897663316678 4.059973275115794 >50 0.017921267731257143 0.9293713494888387 >100 0.01278180906248686 1.8755694579962627 >500 0.0017186767723701775 0.9319307698306606 >1k 0.0011088237241047374 1.6514524001302453 >5k 5.5441186205236864E-5 0.20991165349731672 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5127 0.20758127128649376 No Hit CTGTAGGACGTGGAATATGGCAAGA 4491 0.18183099070560627 No Hit GTATCAACGCAGAGTACTTTTTTTT 4389 0.1777012287256526 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3830 0.15506851356100468 No Hit CTTTAGGACGTGAAATATGGCGAGG 3433 0.1389948321292243 No Hit GGTATCAACGCAGAGTACTTTTTTT 2730 0.11053186475758296 No Hit TATCAACGCAGAGTACTTTTTTTTT 2478 0.10032892339534455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.048786254856519E-5 0.0 4 0.0 0.0 0.0 4.048786254856519E-5 0.0 5 4.048786254856519E-5 0.0 0.0 4.048786254856519E-5 0.0 6 4.048786254856519E-5 0.0 0.0 4.048786254856519E-5 4.048786254856519E-5 7 4.048786254856519E-5 0.0 0.0 4.048786254856519E-5 4.048786254856519E-5 8 4.048786254856519E-5 0.0 0.0 4.048786254856519E-5 4.048786254856519E-5 9 4.048786254856519E-5 0.0 0.0 8.097572509713039E-5 8.097572509713039E-5 10 4.048786254856519E-5 0.0 0.0 8.097572509713039E-5 8.097572509713039E-5 11 4.048786254856519E-5 0.0 0.0 8.097572509713039E-5 8.097572509713039E-5 12 4.048786254856519E-5 0.0 0.0 8.097572509713039E-5 8.097572509713039E-5 13 4.048786254856519E-5 0.0 0.0 8.097572509713039E-5 8.097572509713039E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 285 0.0 13.23079 18 CGTTAGG 55 0.001832259 12.951115 1 GCCGATC 60 4.3734052E-4 12.567723 8 GGCGAGG 1130 0.0 12.430209 19 TAGGACC 845 0.0 12.27051 4 ACCGTAT 70 1.1742648E-4 12.1188755 8 CCAACGA 190 0.0 11.907711 19 TGTAGGA 2540 0.0 11.658189 2 CACCGTA 60 0.0062295245 10.99698 7 GTAGGAC 2625 0.0 10.988003 3 GGACGTG 2650 0.0 10.9554825 6 AGGACGT 2710 0.0 10.921618 5 TGGCGAG 2565 0.0 10.878648 18 CTGTAGG 2590 0.0 10.804501 1 GACGGTG 70 0.0015924992 10.772552 7 GACGTGA 1440 0.0 10.669689 7 CATACGT 80 4.051215E-4 10.605305 19 GTGTAGG 505 0.0 10.478129 1 GACGTGG 1350 0.0 10.473314 7 GGTATCA 1365 0.0 10.436796 1 >>END_MODULE