FastQCFastQC Report
Thu 26 May 2016
SRR1512242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469876
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58910.23851399827359757No Hit
GTCCTACAGTGGACATTTCTAAATT52420.21223737547957874No Hit
CTGTAGGACGTGGAATATGGCAAGA49300.19960516236442638No Hit
GTCCTAAAGTGTGTATTTCTCATTT39760.1609797414930952No Hit
TATCAACGCAGAGTACTTTTTTTTT39070.15818607897724418No Hit
CTTTAGGACGTGAAATATGGCGAGG38460.1557163193617817No Hit
GGTATCAACGCAGAGTACTTTTTTT38260.15490656211081041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC400.00527862414.2506693
TAGGACC8900.012.7028434
CTAAGAC2050.011.1224733
GGCGAGG10800.010.37738419
TGTAGGA26650.010.3381962
GGACCTG17400.010.3161086
GTAGGAC26050.010.2480813
AGGACCT18100.010.2352875
GGACGTG24350.010.1799316
GTCCTAC25850.010.1432131
AGGACGT24950.010.0906535
GACGTGG13200.010.0729677
CTGTAGG25800.010.0150481
CGTGGAA13450.09.9563499
GGTATCA15500.09.8421891
GTAGAAC7500.09.7537913
TCCTACA28750.09.7482832
TGGCGAG25100.09.64872118
GTATAGC4250.09.6467931
ACGTGGA13900.09.5656958
Produced by FastQC (version 0.11.5)