FastQCFastQC Report
Thu 26 May 2016
SRR1512241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115471
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72580.3430914439384894No Hit
CTGTAGGACGTGGAATATGGCAAGA61600.2911881089364969No Hit
GTCCTAAAGTGTGTATTTCTCATTT55400.2618802148552261No Hit
CTTTAGGACGTGAAATATGGCGAGG48650.22997242694416517No Hit
GTATCAACGCAGAGTACTTTTTTTT39400.18624693980678536No Hit
GTCCTACAGTGTGCATTTCTCATTT30130.142426911075595No Hit
CTGAAGGACCTGGAATATGGCGAGA26020.12299861354752677No Hit
GGTATCAACGCAGAGTACTTTTTTT23770.11236268424383979No Hit
TATCAACGCAGAGTACTTTTTTTTT23330.11028276918000766No Hit
CTGTAGGACCTGGAATATGGCGAGA22870.10810831252236498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG502.3618759E-618.3223381
TAGGACC10150.013.7438844
CGTAGGA450.007291997313.57210352
TCCAACG3000.013.19723518
CCAACGT1551.8189894E-1212.1633519
AGGACCT21450.011.644815
GGACCTG21950.011.3366116
CCAACGA2000.011.31191519
GTCCTAC32400.011.3100861
TGTAGGA32600.011.2719232
GGACGTG34600.011.1691876
CACCTTT7850.011.0474714
AGGACGT36100.010.9661945
GTAGGAC34050.010.9066893
GGCCTAA1403.5199264E-810.9061541
CCGTAGG750.001491083810.8576831
GTATTAA2250.010.8576821
CTGTAGG32350.010.8241191
CGGTTTC2700.010.82287213
GACGTGA17850.010.7194297
Produced by FastQC (version 0.11.5)