FastQCFastQC Report
Thu 26 May 2016
SRR1512241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115471
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70600.3337318261512448No Hit
CTGTAGGACGTGGAATATGGCAAGA67610.3195978578765674No Hit
CTTTAGGACGTGAAATATGGCGAGG54250.2564440732111194No Hit
GTCCTAAAGTGTGTATTTCTCATTT52390.24765170498673816No Hit
GTATCAACGCAGAGTACTTTTTTTT50760.2399465650911783No Hit
TATCAACGCAGAGTACTTTTTTTTT35160.16620412191894857No Hit
GGTATCAACGCAGAGTACTTTTTTT32570.1539609855204822No Hit
CTGAAGGACCTGGAATATGGCGAGA30630.14479045092085877No Hit
GTCCTACAGTGTGCATTTCTCATTT28700.1356671871181406No Hit
CTGTAGGACCTGGAATATGGCGAGA24710.11680613915293568No Hit
TTGTAGAACAGTGTATATCAATGAG23140.10938462403880743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10750.013.6980324
ACCGTGC500.001503041113.2962618
CTAGGAC3650.013.274323
TCCAACG2800.013.22842318
GGTATCA14250.012.1113661
TCGAACT1201.00408215E-811.87334519
CCAACGT1302.6229827E-911.69067919
CCAACGA1950.011.69067819
ACGACAG600.00587570311.0836245
AGGACCT21350.010.9019245
GTATAAG2100.010.8973621
TGTAGGA34650.010.802592
CTGTAGG33700.010.7235541
TGGCCCG803.7709766E-410.6877795
ACCTTTT8200.010.655539515
TTTAGAC1252.240231E-710.6402793
TTGTAGA7100.010.6095791
GACGTGG17100.010.55258757
CTCGAAC1451.45028025E-810.47981218
GTCCTAC32950.010.4756881
Produced by FastQC (version 0.11.5)