##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512241_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2115471 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.083528916255528 31.0 30.0 34.0 26.0 34.0 2 30.30175644100061 31.0 30.0 34.0 26.0 34.0 3 30.377037548612105 31.0 30.0 34.0 26.0 34.0 4 33.982016770733324 35.0 35.0 37.0 30.0 37.0 5 33.492211899855874 35.0 35.0 37.0 28.0 37.0 6 33.404349669648035 36.0 35.0 37.0 28.0 37.0 7 33.194610089195265 36.0 33.0 37.0 27.0 37.0 8 33.06404153023133 35.0 33.0 37.0 26.0 37.0 9 34.45275922005076 38.0 34.0 39.0 27.0 39.0 10 34.20495861205377 37.0 34.0 39.0 27.0 39.0 11 34.18677495460822 38.0 34.0 39.0 27.0 39.0 12 33.975899456905815 37.0 34.0 39.0 25.0 39.0 13 33.95486017061921 37.0 34.0 39.0 25.0 39.0 14 34.8003574617662 38.0 34.0 40.0 25.0 41.0 15 34.72422737064228 38.0 34.0 40.0 25.0 41.0 16 34.605248192955614 38.0 34.0 40.0 25.0 41.0 17 34.51384301651972 38.0 33.0 40.0 24.0 41.0 18 34.43557439454381 38.0 33.0 40.0 24.0 41.0 19 34.46077823803777 38.0 34.0 40.0 23.0 41.0 20 34.3189176311091 38.0 34.0 40.0 22.0 41.0 21 34.16986571784723 38.0 33.0 40.0 19.0 41.0 22 34.25856889553201 38.0 34.0 40.0 19.0 41.0 23 34.11960787928551 38.0 34.0 40.0 18.0 41.0 24 33.965549515923406 38.0 34.0 40.0 18.0 41.0 25 33.47267204324711 38.0 33.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.195656344672404 1101 2 0.25823762571119957 1101 3 0.28210224587757793 1101 4 0.35180353041587153 1101 5 0.28690781520928965 1101 6 0.3139329175272323 1101 7 0.15851091405861695 1101 8 -0.05127922292945897 1101 9 0.12880090123969268 1101 10 0.3106304657713892 1101 11 0.25607851942525883 1101 12 0.2812771115146617 1101 13 0.1338827499775448 1101 14 0.019898857860262353 1101 15 0.010969625034512376 1101 16 0.013741112364108687 1101 17 0.02246846172710093 1101 18 0.10284897281336924 1101 19 0.2252437938787608 1101 20 0.13889881043122188 1101 21 -0.005591800240949851 1101 22 0.053311348608353626 1101 23 -0.0909034367988042 1101 24 -0.14923488850450184 1101 25 -0.19684497777544152 1102 1 0.7041293531454116 1102 2 0.6629440304176022 1102 3 0.6363961001714316 1102 4 0.6264363050486494 1102 5 0.8001168284183038 1102 6 0.8454569490512611 1102 7 0.7745245300722345 1102 8 0.8965507249004858 1102 9 1.036652309091103 1102 10 0.9047955599364812 1102 11 0.9888642522109947 1102 12 1.1540709843085395 1102 13 1.0748306909254808 1102 14 1.0467053846667866 1102 15 1.1703287843936678 1102 16 1.3590069576299584 1102 17 1.3151572544158938 1102 18 1.3191881891525838 1102 19 1.2495343181692888 1102 20 1.3372930088254193 1102 21 1.056568261919118 1102 22 1.1955394908364951 1102 23 1.066513220617857 1102 24 1.1983064590368429 1102 25 1.256941976011511 1103 1 0.38929795210711404 1103 2 0.4388185390507324 1103 3 0.4437160152499686 1103 4 0.5097542961252586 1103 5 0.4390679156452535 1103 6 0.5022776188029638 1103 7 0.6231980468491969 1103 8 0.579532015640396 1103 9 0.7101524998326738 1103 10 0.7408386675313139 1103 11 0.6670894433672174 1103 12 0.7103542604194004 1103 13 0.7192303525665267 1103 14 0.7071522175274154 1103 15 0.7889057693499666 1103 16 1.0549006233512088 1103 17 0.8022490259904203 1103 18 1.106009605284349 1103 19 1.007761085016746 1103 20 0.965985516828276 1103 21 0.9504765566894804 1103 22 1.2714069503246463 1103 23 1.2369138608460801 1103 24 1.1787202023816192 1103 25 1.4011197969134699 1104 1 0.7541869136295105 1104 2 0.710379796336781 1104 3 0.6861171882811448 1104 4 0.6461523095987758 1104 5 0.8499338886619086 1104 6 0.869716470467182 1104 7 0.9229152301217027 1104 8 0.7141922723998562 1104 9 1.0023879776703737 1104 10 0.7670598184092725 1104 11 1.096244988027749 1104 12 1.1578152653040021 1104 13 1.2019871574184435 1104 14 1.2668424356095258 1104 15 1.3428538545490696 1104 16 1.4457792164401297 1104 17 1.4239764324293844 1104 18 1.2123096543119658 1104 19 1.3241789819134766 1104 20 1.2673092625383546 1104 21 1.380417451171148 1104 22 1.2709870378480232 1104 23 1.4202621748407438 1104 24 1.5492396227662155 1104 25 1.4730737291100837 1105 1 0.1330280765169647 1105 2 0.15212822187768893 1105 3 0.20416276779875986 1105 4 0.1409240708137034 1105 5 0.3242232633468518 1105 6 0.2211949541436553 1105 7 0.2790527038990618 1105 8 0.168512596955253 1105 9 0.3989819510305921 1105 10 0.3622808590381297 1105 11 0.49636957996713704 1105 12 0.31608995310874377 1105 13 0.4547388562493495 1105 14 0.5703138399984198 1105 15 0.49863384191377236 1105 16 0.5866955615377876 1105 17 0.44642235270374897 1105 18 0.6686927899344752 1105 19 0.8110316706559573 1105 20 0.6244652644138355 1105 21 0.6205646721765561 1105 22 0.7739279359225719 1105 23 0.6396124476882861 1105 24 0.7776886292225456 1105 25 0.9312570641677524 1106 1 -0.05612225056413678 1106 2 -0.0794970695001318 1106 3 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-0.7690703080485335 2315 4 -0.865338895767394 2315 5 -0.797354641012781 2315 6 -1.0248629813879901 2315 7 -1.0858074110307 2315 8 -0.9379047589354457 2315 9 -1.0531014093913953 2315 10 -1.028619316112632 2315 11 -0.8421275425256383 2315 12 -1.0999217722083117 2315 13 -1.081426034858552 2315 14 -1.457078272398192 2315 15 -1.161226237845156 2315 16 -1.526129067937461 2315 17 -1.3743148834270613 2315 18 -1.4316500297902692 2315 19 -1.5296330410806789 2315 20 -1.6066672343546458 2315 21 -1.4445764044389549 2315 22 -1.3983044976839523 2315 23 -1.2962600352256146 2315 24 -1.3501413707326932 2315 25 -1.549068477084763 2316 1 -1.0837161363526029 2316 2 -1.1662378541461464 2316 3 -1.2066669126102063 2316 4 -1.2892068660657188 2316 5 -1.2168659902617023 2316 6 -1.3146517626429812 2316 7 -1.4824925666054263 2316 8 -1.3624245313949999 2316 9 -1.5712607610819092 2316 10 -1.563206951835575 2316 11 -1.7436814347786438 2316 12 -1.9800604789877312 2316 13 -1.8089448556459544 2316 14 -2.1507003062627987 2316 15 -2.1465686830174207 2316 16 -2.0155076647631844 2316 17 -2.2732195483423325 2316 18 -2.1316927248815247 2316 19 -1.8796702737418727 2316 20 -1.9158659870758683 2316 21 -2.1688854070361145 2316 22 -2.297795784544334 2316 23 -2.0796112452922273 2316 24 -2.019071171025022 2316 25 -1.8978927018245066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51546.0 3 1.0 4 979.0 5 8089.0 6 9704.0 7 7797.0 8 6248.0 9 5657.0 10 5171.0 11 5184.0 12 4585.0 13 4436.0 14 4329.0 15 4486.0 16 4498.0 17 4824.0 18 5138.0 19 5903.0 20 7155.0 21 9028.0 22 11733.0 23 14590.0 24 17371.0 25 20661.0 26 23673.0 27 27342.0 28 31751.0 29 38643.0 30 48209.0 31 61029.0 32 77758.0 33 99001.0 34 126825.0 35 165514.0 36 235478.0 37 384657.0 38 576478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.086935144616646 20.87791302498994 14.72491894026258 24.310232890130834 2 14.261504884727799 21.259000950615725 42.17330325019819 22.306190914458295 3 19.044600469588097 25.225824414515728 30.502190765082577 25.227384350813605 4 12.681573039762775 18.219063272434365 38.66557376584222 30.433789921960642 5 12.575708786498504 38.61622140987031 36.32886918060739 12.479200623023793 6 31.17844678560945 38.358266315161025 18.085948708349108 12.377338190880423 7 27.752590321493415 31.791785375455394 22.34632382103087 18.109300482020316 8 23.991820261303513 39.61708763674851 20.357121416459975 16.033970685488008 9 25.743108745050158 15.893529147882434 20.927396310325218 37.435965796742195 10 14.711333788078399 30.159902924691472 34.7165241215786 20.412239165651528 11 33.75550882049435 22.52340022623803 25.60881241104227 18.11227854222535 12 23.526013828598927 24.842127356035608 32.488462380245345 19.14339643512012 13 29.081608776485236 22.578848871007924 26.189817775805007 22.149724576701832 14 19.87302118535305 23.718500513597206 28.036120561331256 28.372357739718485 15 21.933177056078765 33.4083048172251 23.38921214235506 21.269305984341074 16 20.78851470901752 29.111909357301517 29.60518012300807 20.494395810672895 17 20.548237248348002 30.114806584443844 28.852250869900843 20.48470529730731 18 20.25870361730319 28.259096910333444 31.942957383958465 19.539242088404897 19 23.108139983956292 27.339585368932024 28.094594537103085 21.4576801100086 20 23.014638347677657 28.436456940322035 29.06936564008677 19.479539071913536 21 21.941449445537188 27.56435800821661 27.758830066684915 22.73536247956129 22 22.299289378110124 29.58972257242004 28.169140583822706 19.94184746564713 23 21.188567463226864 29.17615037029579 28.74144812195488 20.89383404452247 24 22.64592613181651 27.553958432897453 29.65486173055551 20.145253704730532 25 21.94341346819779 28.520277652106618 29.19653818898326 20.339770690712324 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 220.0 1 220.0 2 338.0 3 456.0 4 456.0 5 456.0 6 1143.0 7 1830.0 8 1830.0 9 1830.0 10 2863.0 11 3896.0 12 3896.0 13 3896.0 14 6906.0 15 9916.0 16 9916.0 17 9916.0 18 18023.0 19 26130.0 20 26130.0 21 26130.0 22 43695.5 23 61261.0 24 61261.0 25 61261.0 26 90388.5 27 119516.0 28 119516.0 29 119516.0 30 157839.5 31 196163.0 32 196163.0 33 196163.0 34 216998.5 35 237834.0 36 237834.0 37 237834.0 38 248992.5 39 260151.0 40 260151.0 41 260151.0 42 269919.5 43 279688.0 44 279688.0 45 279688.0 46 277616.5 47 275545.0 48 275545.0 49 275545.0 50 256715.0 51 237885.0 52 237885.0 53 237885.0 54 203334.0 55 168783.0 56 168783.0 57 168783.0 58 140629.5 59 112476.0 60 112476.0 61 112476.0 62 89584.5 63 66693.0 64 66693.0 65 66693.0 66 50377.5 67 34062.0 68 34062.0 69 34062.0 70 24372.0 71 14682.0 72 14682.0 73 14682.0 74 10154.5 75 5627.0 76 5627.0 77 5627.0 78 3773.5 79 1920.0 80 1920.0 81 1920.0 82 1228.5 83 537.0 84 537.0 85 537.0 86 348.5 87 160.0 88 160.0 89 160.0 90 95.5 91 31.0 92 31.0 93 31.0 94 19.5 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39627109045692427 2 0.0 3 0.0 4 0.0 5 0.029496977268891895 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007657869098654626 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2115471.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.90420939974376 #Duplication Level Percentage of deduplicated Percentage of total 1 88.02378749362674 65.05328423087536 2 8.691142653194737 12.846240531294967 3 1.71178762848019 3.795249340292723 4 0.5725219705427955 1.6924713438779473 5 0.27090490068895784 1.0010506253966762 6 0.15728266445450947 0.6974310581277421 7 0.10770724871693833 0.5572013344132823 8 0.07242262561886306 0.42818695112125627 9 0.05619548753292397 0.37377747701585123 >10 0.2888150005320102 3.8920491136428392 >50 0.022839206576331362 1.1870569571260503 >100 0.01967724205902017 3.041066434748964 >500 0.002234489989068878 1.1261954511392818 >1k 0.0023621751313010735 2.913981778137129 >5k 3.192128555812262E-4 1.3947573727900207 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7060 0.3337318261512448 No Hit CTGTAGGACGTGGAATATGGCAAGA 6761 0.3195978578765674 No Hit CTTTAGGACGTGAAATATGGCGAGG 5425 0.2564440732111194 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5239 0.24765170498673816 No Hit GTATCAACGCAGAGTACTTTTTTTT 5076 0.2399465650911783 No Hit TATCAACGCAGAGTACTTTTTTTTT 3516 0.16620412191894857 No Hit GGTATCAACGCAGAGTACTTTTTTT 3257 0.1539609855204822 No Hit CTGAAGGACCTGGAATATGGCGAGA 3063 0.14479045092085877 No Hit GTCCTACAGTGTGCATTTCTCATTT 2870 0.1356671871181406 No Hit CTGTAGGACCTGGAATATGGCGAGA 2471 0.11680613915293568 No Hit TTGTAGAACAGTGTATATCAATGAG 2314 0.10938462403880743 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.418123907158264E-4 2 0.0 0.0 0.0 0.0 1.418123907158264E-4 3 0.0 0.0 0.0 0.0 1.418123907158264E-4 4 0.0 0.0 0.0 0.0 1.418123907158264E-4 5 0.0 0.0 0.0 0.0 1.418123907158264E-4 6 0.0 0.0 0.0 0.0 1.418123907158264E-4 7 0.0 0.0 0.0 0.0 1.418123907158264E-4 8 0.0 0.0 0.0 0.0 1.418123907158264E-4 9 0.0 0.0 0.0 0.0 1.418123907158264E-4 10 0.0 0.0 0.0 0.0 1.418123907158264E-4 11 0.0 0.0 0.0 0.0 1.418123907158264E-4 12 0.0 0.0 0.0 0.0 1.418123907158264E-4 13 0.0 0.0 0.0 4.7270796905275465E-5 1.418123907158264E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1075 0.0 13.698032 4 ACCGTGC 50 0.0015030411 13.296261 8 CTAGGAC 365 0.0 13.27432 3 TCCAACG 280 0.0 13.228423 18 GGTATCA 1425 0.0 12.111366 1 TCGAACT 120 1.00408215E-8 11.873345 19 CCAACGT 130 2.6229827E-9 11.690679 19 CCAACGA 195 0.0 11.690678 19 ACGACAG 60 0.005875703 11.083624 5 AGGACCT 2135 0.0 10.901924 5 GTATAAG 210 0.0 10.897362 1 TGTAGGA 3465 0.0 10.80259 2 CTGTAGG 3370 0.0 10.723554 1 TGGCCCG 80 3.7709766E-4 10.687779 5 ACCTTTT 820 0.0 10.6555395 15 TTTAGAC 125 2.240231E-7 10.640279 3 TTGTAGA 710 0.0 10.609579 1 GACGTGG 1710 0.0 10.5525875 7 CTCGAAC 145 1.45028025E-8 10.479812 18 GTCCTAC 3295 0.0 10.475688 1 >>END_MODULE