FastQCFastQC Report
Thu 26 May 2016
SRR1512240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332569
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58290.43742575431366026No Hit
CTGTAGGACGTGGAATATGGCAAGA50200.3767159524197246No Hit
GTCCTAAAGTGTGTATTTCTCATTT47740.3582553698907899No Hit
CTTTAGGACGTGAAATATGGCGAGG41180.30902714981363066No Hit
GTATCAACGCAGAGTACTTTTTTTT38800.29116691143197837No Hit
GGTATCAACGCAGAGTACTTTTTTT23170.1738746736566737No Hit
CTGAAGGACCTGGAATATGGCGAGA22160.16629532879723302No Hit
TATCAACGCAGAGTACTTTTTTTTT22070.16561994163154029No Hit
GTCCTACAGTGTGCATTTCTCATTT21280.15969154317712628No Hit
GTCCTTCAGTGTGCATTTCTCATTT17030.12779826035274722No Hit
CTGTAGGACCTGGAATATGGCGAGA16750.1256970558372587No Hit
TTTCTAAATTTTCCACCTTTTTCAG16620.12472149659792475No Hit
ATTTAGAAATGTCCACTGTAGGACG15960.11976865738284471No Hit
GAATATGGCAAGAAAACTGAAAATC15220.11421547402048224No Hit
GGAATATGGCGAGAAAACTGAAAAT15040.11286469968909679No Hit
TTGTAGAACAGTGTATATCAATGAG14010.10513526879283548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG454.203265E-415.7322571
GACGTTT552.0814687E-413.72040212
GCGCCAC552.0814687E-413.72040213
CCAACGA1450.013.661262519
CGGTTTC1205.820766E-1113.36310113
GCCGGTT1154.2200554E-1013.12386411
TCCAACG1950.013.06076818
TAGGACC7550.012.9930054
TGCCGGT1201.1115844E-811.79097210
TAGTACC803.0827505E-511.7905284
GGCGAGG13250.011.67528619
ACCTTTT6700.011.26301815
TGTAGGA27650.011.1926222
CCGGTTT1355.2787072E-911.17958712
CCACCTT6650.011.06400913
TGTCGAG1451.36788E-911.05911718
GGTATCA7800.011.0212251
GGACGTA600.006195421311.0044936
CACCTTT6550.010.94490114
CTGTAGG27250.010.9115471
Produced by FastQC (version 0.11.5)