##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512240_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1332569 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.004085341922256 31.0 30.0 34.0 25.0 34.0 2 27.260293463227796 31.0 28.0 34.0 10.0 34.0 3 28.857246416508264 31.0 28.0 34.0 16.0 34.0 4 33.39878310241346 35.0 33.0 37.0 28.0 37.0 5 33.79885694474358 37.0 35.0 37.0 30.0 37.0 6 34.01743699575782 37.0 35.0 37.0 30.0 37.0 7 34.042677715000124 37.0 35.0 37.0 30.0 37.0 8 34.04785868499117 37.0 35.0 37.0 30.0 37.0 9 35.63924269587541 39.0 37.0 39.0 30.0 39.0 10 35.50571114891611 39.0 35.0 39.0 30.0 39.0 11 35.52826532809933 39.0 37.0 39.0 30.0 39.0 12 35.43888158887082 39.0 35.0 39.0 30.0 39.0 13 35.44197186036896 39.0 35.0 39.0 30.0 39.0 14 36.57269004456805 40.0 37.0 41.0 30.0 41.0 15 36.5302922400266 40.0 37.0 41.0 30.0 41.0 16 36.487706827939114 40.0 37.0 41.0 30.0 41.0 17 36.49590902985136 40.0 37.0 41.0 30.0 41.0 18 36.45201336666244 40.0 37.0 41.0 30.0 41.0 19 36.49150250381031 40.0 37.0 41.0 30.0 41.0 20 36.42729494682827 40.0 37.0 41.0 30.0 41.0 21 36.35714998622961 40.0 37.0 41.0 30.0 41.0 22 36.2782362489297 40.0 37.0 41.0 30.0 41.0 23 36.170265854901324 40.0 37.0 41.0 29.0 41.0 24 36.09566108771853 40.0 37.0 41.0 29.0 41.0 25 35.981020119783665 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5437926301741243 1101 2 3.6778559100232684 1101 3 2.0422498573416696 1101 4 1.016569715357413 1101 5 0.6548629979642868 1101 6 0.6475710268196053 1101 7 0.3892459806753976 1101 8 0.4166891671582178 1101 9 0.296358056449435 1101 10 0.42268023096343654 1101 11 0.4681377569033174 1101 12 0.4774711847743376 1101 13 0.5394098028758947 1101 14 0.4241293425969985 1101 15 0.43876816965573795 1101 16 0.5910736579484777 1101 17 0.46780034347200683 1101 18 0.49632609513099624 1101 19 0.42298454656583573 1101 20 0.3579708106754609 1101 21 0.586365324010913 1101 22 0.47880801240172843 1101 23 0.5482512750943442 1101 24 0.4394964420869769 1101 25 0.566863999476297 1102 1 1.288981760883619 1102 2 4.221399852551066 1102 3 2.589207248176507 1102 4 1.7018476836314278 1102 5 1.189396815527111 1102 6 1.1248783885097637 1102 7 1.0463427714645874 1102 8 0.9939275506202208 1102 9 1.0460704806720358 1102 10 1.093289462058479 1102 11 1.124557252418235 1102 12 1.2494829065778035 1102 13 1.1307894723298304 1102 14 1.096606629979476 1102 15 1.251226306963872 1102 16 1.2758881085126674 1102 17 1.2135294123758058 1102 18 1.1575016419745054 1102 19 1.0554829319957975 1102 20 1.296833424126163 1102 21 1.446002871143122 1102 22 1.3731449548131494 1102 23 1.282166136680864 1102 24 1.2527322702078152 1102 25 1.281375050727469 1103 1 1.0591279392236537 1103 2 4.071599141944837 1103 3 2.521582615466489 1103 4 1.5138099649478889 1103 5 1.1598090409048538 1103 6 0.9811554511734357 1103 7 0.7235996838928145 1103 8 0.8893740154157115 1103 9 0.8125706835267508 1103 10 0.9537067280686387 1103 11 0.841167980359323 1103 12 0.9331593717421143 1103 13 1.0269470351225891 1103 14 1.0265782303187976 1103 15 1.1792450925402846 1103 16 1.0368942679195925 1103 17 1.1562043899388499 1103 18 1.0438975897978153 1103 19 1.2926528013081793 1103 20 1.3138963336775475 1103 21 1.2231916735626243 1103 22 1.2757323974913248 1103 23 1.17955312201385 1103 24 1.1710381259473763 1103 25 1.21357043055756 1104 1 1.0320519158318398 1104 2 4.025933937266476 1104 3 2.4627302762852032 1104 4 1.3988611345385351 1104 5 1.1734640116650894 1104 6 0.8142767962026696 1104 7 0.7105148885711827 1104 8 0.7292936060589881 1104 9 0.675787057795759 1104 10 0.7175152076007976 1104 11 0.7630247055055221 1104 12 0.7973625881163855 1104 13 0.6129704269354548 1104 14 0.923785832657984 1104 15 0.6031193615461348 1104 16 0.7256661977441539 1104 17 0.8263578987107749 1104 18 0.7635832623124301 1104 19 0.8941732691444386 1104 20 0.8117325909874893 1104 21 0.8246755916912747 1104 22 0.9379253799474654 1104 23 0.9619946424816845 1104 24 1.1278656113274934 1104 25 0.9220280328967476 1105 1 0.9342224763993485 1105 2 3.849617818059137 1105 3 2.4230418847361257 1105 4 1.510783314351933 1105 5 1.0472303005077137 1105 6 0.7894420942210232 1105 7 0.6142555393538132 1105 8 0.5920687814772876 1105 9 0.5923303533466893 1105 10 0.6334267081019931 1105 11 0.4984698053590151 1105 12 0.4731659461239417 1105 13 0.658081846474353 1105 14 0.7187671342232491 1105 15 0.7390907163151965 1105 16 0.5104221542558349 1105 17 0.5451165732423533 1105 18 0.5011521766466132 1105 19 0.5788906721824532 1105 20 0.6561654693898333 1105 21 0.6163996066500275 1105 22 0.6817433304448102 1105 23 0.5011562971967791 1105 24 0.7308024221841549 1105 25 0.8971634519730003 1106 1 0.20166350449786918 1106 2 3.1456669044743713 1106 3 1.5339357044927802 1106 4 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-1.3872420607377194 2315 18 -1.3233743613696447 2315 19 -1.7784139165814352 2315 20 -1.512963272759471 2315 21 -1.6197536459405129 2315 22 -1.3728815949106021 2315 23 -1.4775126567883845 2315 24 -1.448949068315649 2315 25 -1.3587657627236922 2316 1 -1.3562287005509646 2316 2 0.03632019030783695 2316 3 -0.3908534625631326 2316 4 -1.2709938938475176 2316 5 -1.1380728902002772 2316 6 -1.505375474681351 2316 7 -1.3974230676331985 2316 8 -1.3596104896425558 2316 9 -1.406897451531087 2316 10 -1.4069728297065822 2316 11 -2.040472861404453 2316 12 -1.7455054167497934 2316 13 -1.4763645284579 2316 14 -1.6541440132463165 2316 15 -1.7549402491594606 2316 16 -1.7013411015259194 2316 17 -1.8454740153200433 2316 18 -2.2438934205748353 2316 19 -1.8270757170680554 2316 20 -1.9355099069849402 2316 21 -1.9084803288277783 2316 22 -1.8943738901255287 2316 23 -1.817334230186603 2316 24 -1.6517065703432294 2316 25 -1.5363813344998505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40011.0 3 1.0 4 319.0 5 1754.0 6 2074.0 7 1780.0 8 1636.0 9 1536.0 10 1594.0 11 1402.0 12 1345.0 13 1416.0 14 1427.0 15 1509.0 16 1597.0 17 1644.0 18 1844.0 19 2400.0 20 3081.0 21 3824.0 22 4843.0 23 5654.0 24 6695.0 25 7764.0 26 8746.0 27 10283.0 28 11943.0 29 14818.0 30 19125.0 31 25427.0 32 34697.0 33 48702.0 34 70332.0 35 106714.0 36 162182.0 37 218886.0 38 503564.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.407213435101674 20.2273953543869 14.035896077426385 25.32949513308504 2 14.040121529677574 20.91325883632759 43.67464741588512 21.371972218109725 3 18.03553887265875 25.045457308402042 30.77296560253165 26.146038216407558 4 11.293749141695477 17.718632205912037 40.130379740186065 30.857238912206427 5 11.624163551756043 38.63207083460593 37.422227291795025 12.321538321842997 6 29.21708943496982 38.988913119500275 19.261034725484517 12.532962720045388 7 26.179853280549352 32.1851441865701 23.580630030582185 18.054372502298357 8 22.526863524515427 40.24879762323752 21.59107708493894 15.63326176730811 9 25.285820096370244 15.90064004190402 22.126884236388513 36.686655625337224 10 14.32490968227071 29.773181300365177 35.93640010866645 19.965508908697668 11 32.11900898886186 22.55402847798712 27.395466395705036 17.931496137445986 12 23.193095441962104 24.700259423714645 33.79412248071207 18.312522653611182 13 28.21264790040891 23.302658248841148 26.877407473834374 21.607286376915567 14 18.76991932088764 24.53505012520984 28.835859918067662 27.85917063583485 15 20.655665860454505 34.89132645288912 23.995305308768252 20.457702377888122 16 19.10992976723907 29.0799951071952 31.912794009165758 19.897281116399977 17 19.219131781642663 30.5260219365916 30.47214100893913 19.782705272826604 18 18.874847646094636 28.060587320552617 34.368264156154495 18.69630087719825 19 22.125922679530397 27.193868514977503 29.196447064685877 21.483761740806226 20 21.91203607467981 28.8236481563056 30.67113222654887 18.59318354246572 21 20.401007735125063 28.07586981695213 28.725854469779577 22.797267978143225 22 21.09847953286591 30.725653277060317 29.238355605151323 18.937511584922454 23 19.49557911294087 30.033514991692545 30.465322564909968 20.005583330456616 24 21.664605279353005 27.653785997976637 31.564217718524567 19.11739100414579 25 20.58569247880259 28.85257048167544 30.960529060979802 19.60120797854217 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 312.0 1 312.0 2 374.0 3 436.0 4 436.0 5 436.0 6 1043.0 7 1650.0 8 1650.0 9 1650.0 10 2389.5 11 3129.0 12 3129.0 13 3129.0 14 5170.0 15 7211.0 16 7211.0 17 7211.0 18 13435.0 19 19659.0 20 19659.0 21 19659.0 22 32586.0 23 45513.0 24 45513.0 25 45513.0 26 66921.5 27 88330.0 28 88330.0 29 88330.0 30 116028.0 31 143726.0 32 143726.0 33 143726.0 34 156129.5 35 168533.0 36 168533.0 37 168533.0 38 171798.5 39 175064.0 40 175064.0 41 175064.0 42 176752.0 43 178440.0 44 178440.0 45 178440.0 46 173275.0 47 168110.0 48 168110.0 49 168110.0 50 151614.5 51 135119.0 52 135119.0 53 135119.0 54 111141.0 55 87163.0 56 87163.0 57 87163.0 58 71190.0 59 55217.0 60 55217.0 61 55217.0 62 43013.0 63 30809.0 64 30809.0 65 30809.0 66 22914.0 67 15019.0 68 15019.0 69 15019.0 70 10540.5 71 6062.0 72 6062.0 73 6062.0 74 4078.5 75 2095.0 76 2095.0 77 2095.0 78 1398.0 79 701.0 80 701.0 81 701.0 82 446.0 83 191.0 84 191.0 85 191.0 86 122.0 87 53.0 88 53.0 89 53.0 90 36.5 91 20.0 92 20.0 93 20.0 94 12.5 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 6.734735687232705 3 0.0 4 0.0 5 0.0 6 2.2512905523091112E-4 7 0.0070540437305685495 8 0.0 9 0.0 10 0.0038271939389254886 11 0.002326333570719415 12 0.0 13 0.0 14 4.5025811046182224E-4 15 0.0 16 0.0 17 7.504301841030371E-5 18 0.010881237669494039 19 0.002776591681181237 20 0.0 21 0.005703269399183082 22 0.0013507743313854667 23 0.002326333570719415 24 0.009980721448570392 25 0.007129086748978852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1332569.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.14411837016407 #Duplication Level Percentage of deduplicated Percentage of total 1 91.53390791420156 69.69768719102662 2 6.186141869270759 9.420766374967613 3 1.0416357838163814 2.3794331526453947 4 0.37407855185382277 1.1393552612838815 5 0.20546617892106844 0.7822520524415573 6 0.12837893947226145 0.5865180698047203 7 0.08031332915401697 0.42807713492637917 8 0.0661303793595454 0.4028351547053664 9 0.044212097090749615 0.3029842038844102 >10 0.28412400561147766 4.021335730182248 >50 0.02759416682060925 1.4961857412881645 >100 0.022433409881553013 3.5364326719296977 >500 0.0030907966237950267 1.7112689757745527 >1k 0.002293171688622117 3.271233259267544 >5k 1.9940623379322752E-4 0.8236350258718833 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5829 0.43742575431366026 No Hit CTGTAGGACGTGGAATATGGCAAGA 5020 0.3767159524197246 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4774 0.3582553698907899 No Hit CTTTAGGACGTGAAATATGGCGAGG 4118 0.30902714981363066 No Hit GTATCAACGCAGAGTACTTTTTTTT 3880 0.29116691143197837 No Hit GGTATCAACGCAGAGTACTTTTTTT 2317 0.1738746736566737 No Hit CTGAAGGACCTGGAATATGGCGAGA 2216 0.16629532879723302 No Hit TATCAACGCAGAGTACTTTTTTTTT 2207 0.16561994163154029 No Hit GTCCTACAGTGTGCATTTCTCATTT 2128 0.15969154317712628 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1703 0.12779826035274722 No Hit CTGTAGGACCTGGAATATGGCGAGA 1675 0.1256970558372587 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1662 0.12472149659792475 No Hit ATTTAGAAATGTCCACTGTAGGACG 1596 0.11976865738284471 No Hit GAATATGGCAAGAAAACTGAAAATC 1522 0.11421547402048224 No Hit GGAATATGGCGAGAAAACTGAAAAT 1504 0.11286469968909679 No Hit TTGTAGAACAGTGTATATCAATGAG 1401 0.10513526879283548 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 7.504301841030371E-5 9 0.0 0.0 0.0 0.0 7.504301841030371E-5 10 0.0 0.0 0.0 0.0 7.504301841030371E-5 11 0.0 1.5008603682060742E-4 0.0 0.0 7.504301841030371E-5 12 0.0 1.5008603682060742E-4 0.0 0.0 7.504301841030371E-5 13 0.0 1.5008603682060742E-4 0.0 7.504301841030371E-5 7.504301841030371E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 45 4.203265E-4 15.732257 1 GACGTTT 55 2.0814687E-4 13.720402 12 GCGCCAC 55 2.0814687E-4 13.720402 13 CCAACGA 145 0.0 13.6612625 19 CGGTTTC 120 5.820766E-11 13.363101 13 GCCGGTT 115 4.2200554E-10 13.123864 11 TCCAACG 195 0.0 13.060768 18 TAGGACC 755 0.0 12.993005 4 TGCCGGT 120 1.1115844E-8 11.790972 10 TAGTACC 80 3.0827505E-5 11.790528 4 GGCGAGG 1325 0.0 11.675286 19 ACCTTTT 670 0.0 11.263018 15 TGTAGGA 2765 0.0 11.192622 2 CCGGTTT 135 5.2787072E-9 11.179587 12 CCACCTT 665 0.0 11.064009 13 TGTCGAG 145 1.36788E-9 11.059117 18 GGTATCA 780 0.0 11.021225 1 GGACGTA 60 0.0061954213 11.004493 6 CACCTTT 655 0.0 10.944901 14 CTGTAGG 2725 0.0 10.911547 1 >>END_MODULE