FastQCFastQC Report
Thu 26 May 2016
SRR1512240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332569
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54870.4117610420173365No Hit
CTGTAGGACGTGGAATATGGCAAGA48800.3662099298422821No Hit
GTCCTACAGTGGACATTTCTAAATT41290.309852623016144No Hit
CTTTAGGACGTGAAATATGGCGAGG41010.3077514185006555No Hit
GTCCTAAAGTGTGTATTTCTCATTT39170.29394350311315964No Hit
TATCAACGCAGAGTACTTTTTTTTT38990.29259272878177417No Hit
GGTATCAACGCAGAGTACTTTTTTT38600.2896660510637723No Hit
CTGAAGGACCTGGAATATGGCGAGA23790.17852734079811253No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20230.15181202624404438No Hit
TTTCTAAATTTTCCACCTTTTTCAG20200.1515868971888135No Hit
ATTTAGAAATGTCCACTGTAGGACG17780.13342648673351998No Hit
GAATATGGCAAGAAAACTGAAAATC17330.13004955090505632No Hit
CTGTAGGACCTGGAATATGGCGAGA16570.12434628150587324No Hit
GGAATATGGCGAGAAAACTGAAAAT16550.12419619546905263No Hit
TTGTAGAACAGTGTATATCAATGAG16020.12021891549330653No Hit
GTCCTACAGTGTGCATTTCTCATTT15360.11526607627822649No Hit
ACAGTGGACATTTCTAAATTTTCCA14640.10986297895268463No Hit
ATTCCAGGTCCTTCAGTGTGCATTT13980.10491013973760459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2000.014.24662318
TAGGACC7700.013.2001514
CCAACGA1207.421477E-1012.66461519
CACCTTT6900.012.38836814
ACCTTTT7450.012.36619715
CCACCTT6400.012.31739313
GGTATCA11800.012.0389971
CCAACGT1052.724264E-711.7619
ATCCCGT1302.6229827E-911.68953710
AGGACCT15950.011.4942985
GGACCTG15300.011.4842056
CCCGTTT1354.7402864E-911.25659112
TCCCGTT1354.7402864E-911.25659111
CCTTTTT7950.011.23004316
AATCCCG2800.011.19461519
TTCCACC6750.011.11588411
AAATGTC6700.011.057087
AATGTCC6550.011.0202898
AGGACGG1303.2674507E-810.9605875
TCCACCT7300.010.92891712
Produced by FastQC (version 0.11.5)