##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512240_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1332569 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.474693618116586 31.0 28.0 31.0 22.0 34.0 2 28.708052641176554 31.0 28.0 33.0 23.0 34.0 3 28.71786826798462 31.0 28.0 33.0 23.0 34.0 4 32.233007071303625 35.0 32.0 37.0 25.0 37.0 5 31.598904071759137 35.0 32.0 37.0 20.0 37.0 6 31.551614212847515 35.0 32.0 37.0 19.0 37.0 7 31.280362968071447 35.0 32.0 37.0 17.0 37.0 8 31.10173582005885 35.0 32.0 37.0 17.0 37.0 9 32.22573990540077 37.0 32.0 39.0 16.0 39.0 10 31.89231627030195 37.0 31.0 39.0 11.0 39.0 11 31.900316606494673 37.0 32.0 39.0 10.0 39.0 12 31.62052696708388 37.0 31.0 39.0 10.0 39.0 13 31.59201512266907 37.0 31.0 39.0 10.0 39.0 14 32.13289443173299 37.0 31.0 40.0 10.0 41.0 15 32.044024737180585 37.0 31.0 40.0 8.0 41.0 16 31.871927832630057 37.0 31.0 40.0 7.0 41.0 17 31.7570880006964 37.0 31.0 40.0 2.0 41.0 18 31.63883971486655 37.0 30.0 40.0 2.0 41.0 19 31.665750891698668 37.0 30.0 40.0 2.0 41.0 20 31.492196651730605 37.0 30.0 40.0 2.0 41.0 21 31.292496673718208 37.0 30.0 40.0 2.0 41.0 22 31.349786765263186 37.0 30.0 40.0 2.0 41.0 23 31.13333118210014 37.0 30.0 40.0 2.0 41.0 24 30.909565658513742 37.0 30.0 40.0 2.0 41.0 25 30.354775625127104 36.0 29.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41822518050909707 1101 2 0.4636481877122094 1101 3 0.4713673676515775 1101 4 0.5848370113709009 1101 5 0.7107272499189676 1101 6 0.41477720040014177 1101 7 0.44833413047495085 1101 8 -0.016661354465270506 1101 9 0.2823196403945545 1101 10 0.5928340099382865 1101 11 0.3813273096218346 1101 12 0.5870321513413685 1101 13 0.5159181697156257 1101 14 0.5582473174021771 1101 15 0.10379611825144153 1101 16 0.38771509285582795 1101 17 0.3410343257016386 1101 18 0.4594204172013505 1101 19 0.33502974345351433 1101 20 0.34283123406847693 1101 21 0.18129030277416192 1101 22 0.29352000866397177 1101 23 0.07376837488654786 1101 24 0.06832928093700374 1101 25 0.266542176168727 1102 1 0.5271038922095705 1102 2 0.6526690115450116 1102 3 0.5353632799048782 1102 4 0.6389127455694634 1102 5 0.8637255551823344 1102 6 0.9243464935506225 1102 7 0.8672552430573113 1102 8 0.6869899963042556 1102 9 0.9806290494544285 1102 10 0.9451489262678479 1102 11 1.0389350687484615 1102 12 1.2629448641636785 1102 13 1.2542124744915029 1102 14 1.381968735266888 1102 15 1.393213479487077 1102 16 1.315796424230534 1102 17 1.4289533459610588 1102 18 1.4428406684299446 1102 19 1.2737335221717565 1102 20 1.4041359465151722 1102 21 1.1367411561612109 1102 22 1.4456577093863707 1102 23 1.257164551165129 1102 24 1.3938171108499766 1102 25 1.1823108188959672 1103 1 0.4758315628226839 1103 2 0.5015464258235234 1103 3 0.683738352133453 1103 4 0.70535319848873 1103 5 0.6924006065107307 1103 6 0.7607251065161122 1103 7 0.8189659845220412 1103 8 0.9020646837235731 1103 9 0.9085981663602212 1103 10 1.0101022696824487 1103 11 0.6668518122540341 1103 12 0.8998860608835741 1103 13 0.8041056376727553 1103 14 0.7292973230292148 1103 15 0.9446139147026535 1103 16 1.0774281138259312 1103 17 0.5695176509070272 1103 18 0.9025635565893779 1103 19 1.0139673583389133 1103 20 0.7836090160144273 1103 21 0.9850161533950121 1103 22 1.0369179904323609 1103 23 1.2885502333102288 1103 24 1.0313808871442554 1103 25 0.9107421986768856 1104 1 0.35655524703321007 1104 2 0.36223356032644816 1104 3 0.3257632059346207 1104 4 0.4174511517051016 1104 5 0.3653903725926 1104 6 0.34764031119447125 1104 7 0.4948797272121013 1104 8 0.34071965448380936 1104 9 0.7720776985239581 1104 10 0.7915861878110988 1104 11 0.863533098803746 1104 12 0.8381755345677888 1104 13 0.9532503745148659 1104 14 1.2075721768303893 1104 15 1.274570055053534 1104 16 1.3976620319545887 1104 17 0.9373027386263253 1104 18 1.2784772992794373 1104 19 0.9147641419646426 1104 20 1.2087405949617995 1104 21 1.0762222937458894 1104 22 1.0053463530054607 1104 23 0.7358382450061356 1104 24 0.8594876122904545 1104 25 0.8742582805482293 1105 1 0.402497012011235 1105 2 0.4038472159668238 1105 3 0.38821453217411417 1105 4 0.6994654743205686 1105 5 0.4681435725309129 1105 6 0.424779065146204 1105 7 0.5929216926681917 1105 8 0.4556437041406838 1105 9 0.6749441492911785 1105 10 0.8189104803548588 1105 11 0.7249381801515717 1105 12 0.5714192310748416 1105 13 0.8675474483062118 1105 14 0.9526816686956039 1105 15 1.0079120629184395 1105 16 1.1740364632908467 1105 17 0.6287250726536193 1105 18 0.8156818832874535 1105 19 1.2093112350327253 1105 20 0.9410937062271252 1105 21 1.1238729414442439 1105 22 1.3118469505843073 1105 23 1.3452477021732285 1105 24 1.5231032534347229 1105 25 1.340462787449301 1106 1 -0.14408348267070537 1106 2 -0.28208751576396907 1106 3 -0.2456292646161593 1106 4 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-0.714537591559381 2314 3 -0.5389093220979966 2314 4 -0.4575552193448438 2314 5 -0.7904901065103545 2314 6 -0.5779840517005326 2314 7 -0.6326113533179694 2314 8 -0.47192050861507084 2314 9 -0.7761129232905155 2314 10 -0.60551498446209 2314 11 -0.665964862460271 2314 12 -0.5683611626799205 2314 13 -1.0729537539255034 2314 14 -0.802952797685414 2314 15 -0.5667525749158884 2314 16 -0.6067212504103487 2314 17 -0.8439090350739846 2314 18 -0.5187385518928345 2314 19 -0.6505200068630828 2314 20 -0.5881630747629707 2314 21 -0.8272371863764363 2314 22 -0.8525218936815904 2314 23 -0.7939718977053829 2314 24 -0.9804652419399247 2314 25 -1.0681665410623715 2315 1 -0.5200313304201565 2315 2 -0.5265032604359199 2315 3 -0.4377556505130684 2315 4 -0.8268361556752701 2315 5 -0.6929581675533854 2315 6 -0.8822765582296981 2315 7 -0.6548718948479149 2315 8 -0.6392884558162741 2315 9 -0.7845524717923418 2315 10 -0.85600606685232 2315 11 -0.8679652791362393 2315 12 -0.8796367119853343 2315 13 -0.8593459352985988 2315 14 -1.0920831354161642 2315 15 -0.988517941702348 2315 16 -1.0519730045417646 2315 17 -1.0986208217954143 2315 18 -1.1766271208341053 2315 19 -1.293320813428707 2315 20 -1.2246839792474482 2315 21 -1.3021789633506238 2315 22 -1.1561652041721544 2315 23 -1.3500650396653988 2315 24 -1.0415008305319304 2315 25 -1.3323465450803162 2316 1 -1.2694230579140715 2316 2 -1.3310450284813378 2316 3 -1.324211449377625 2316 4 -1.573794793144856 2316 5 -1.6296978269207827 2316 6 -1.6949286263562193 2316 7 -1.8268102565672955 2316 8 -1.47140524413744 2316 9 -1.9086400630332179 2316 10 -2.027944428571704 2316 11 -2.102352951480114 2316 12 -1.9818264930072331 2316 13 -1.9388268760934082 2316 14 -2.2583442870787778 2316 15 -2.1880313236974835 2316 16 -2.379629127818326 2316 17 -2.2592047634012538 2316 18 -2.333155912399395 2316 19 -2.014326490638762 2316 20 -2.301731830017925 2316 21 -2.243784802766683 2316 22 -2.2980954555914543 2316 23 -2.1683943178486906 2316 24 -1.8728065888449876 2316 25 -1.865193260408784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64704.0 3 3.0 4 1071.0 5 8401.0 6 9771.0 7 7843.0 8 6408.0 9 5657.0 10 5265.0 11 4752.0 12 4638.0 13 4406.0 14 4307.0 15 4214.0 16 4463.0 17 4648.0 18 4895.0 19 5745.0 20 6731.0 21 8745.0 22 11093.0 23 13372.0 24 15945.0 25 18635.0 26 20995.0 27 24057.0 28 27662.0 29 32861.0 30 40661.0 31 50512.0 32 63095.0 33 78814.0 34 98646.0 35 127089.0 36 167021.0 37 214253.0 38 161191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.0956935853047 24.06288388263463 16.20187133252041 21.639551199540264 2 15.278908634374655 24.063219240429575 41.16169594219887 19.4961761829969 3 19.870565801845906 27.787829373188178 31.424714217425137 20.916890607540772 4 14.429046450877966 20.789242433224846 38.34653214955473 26.435178966342455 5 12.099785170939978 40.08258383362533 36.55404279466936 11.263588200765339 6 29.827648699617054 40.36203753801867 18.078913737299906 11.731400025064369 7 26.476602712504942 33.345740445710504 23.368696105042215 16.808960736742335 8 23.510077151727227 41.044778919515615 20.727857244165218 14.717286684591944 9 25.906801073715506 15.972606296559503 21.88704674954918 36.23354588017581 10 14.918251887894735 31.545008175936857 34.87864418277778 18.65809575339063 11 32.9626458367259 23.01089099326189 26.84213725518153 17.184325914830676 12 23.511202797003385 25.920533946084596 33.19895630169995 17.369306955212075 13 29.12419544503887 23.64455424071849 26.617533501079492 20.613716813163148 14 19.582850869260803 25.35846173819142 28.625159372610348 26.43352801993743 15 21.4946468062817 35.46953290974051 23.77745542632314 19.25836485765465 16 19.905911063517163 30.33006170787404 30.65762448323501 19.106402745373785 17 19.89825667563931 31.577051544798056 29.461738941848413 19.06295283771422 18 19.608890796649177 28.984090129666836 33.51143543036045 17.895583643323533 19 22.930144705452403 27.80959184852717 29.37003637335102 19.890227072669408 20 22.78118431390795 29.37656511595272 30.165942626610704 17.676307943528627 21 21.338032026859395 28.36596078702116 28.95414796532112 21.341859220798323 22 22.028577882271012 30.577928797683274 29.378216062357748 18.01527725768797 23 20.38678672549039 30.449004892054372 30.083320263340962 19.08088811911428 24 22.279071477724603 28.639717718181945 30.83907850175113 18.242132302342316 25 21.451015796611035 29.69393643982256 30.36140310588885 18.493644657677557 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 313.0 1 313.0 2 427.0 3 541.0 4 541.0 5 541.0 6 1320.5 7 2100.0 8 2100.0 9 2100.0 10 3062.0 11 4024.0 12 4024.0 13 4024.0 14 6348.5 15 8673.0 16 8673.0 17 8673.0 18 15604.0 19 22535.0 20 22535.0 21 22535.0 22 36424.5 23 50314.0 24 50314.0 25 50314.0 26 73363.5 27 96413.0 28 96413.0 29 96413.0 30 123220.0 31 150027.0 32 150027.0 33 150027.0 34 161378.5 35 172730.0 36 172730.0 37 172730.0 38 173810.5 39 174891.0 40 174891.0 41 174891.0 42 175281.0 43 175671.0 44 175671.0 45 175671.0 46 168770.0 47 161869.0 48 161869.0 49 161869.0 50 145162.5 51 128456.0 52 128456.0 53 128456.0 54 105419.5 55 82383.0 56 82383.0 57 82383.0 58 66844.0 59 51305.0 60 51305.0 61 51305.0 62 39683.0 63 28061.0 64 28061.0 65 28061.0 66 20904.0 67 13747.0 68 13747.0 69 13747.0 70 9670.0 71 5593.0 72 5593.0 73 5593.0 74 3804.0 75 2015.0 76 2015.0 77 2015.0 78 1327.5 79 640.0 80 640.0 81 640.0 82 420.0 83 200.0 84 200.0 85 200.0 86 125.0 87 50.0 88 50.0 89 50.0 90 30.5 91 11.0 92 11.0 93 11.0 94 8.0 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3641087253267936 2 0.0 3 0.0 4 0.0 5 0.026039927388375385 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.005102925251900652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1332569.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.13661509753375 #Duplication Level Percentage of deduplicated Percentage of total 1 92.43249222828946 70.37497083291055 2 5.391807205638636 8.210278997916356 3 0.9721824150030888 2.22056035007043 4 0.35694512865052386 1.0870637548401842 5 0.1856181140927731 0.7066167453905786 6 0.11968605679928315 0.546749474344115 7 0.08447808287705813 0.45023126961316917 8 0.06316394576362926 0.38472712213175514 9 0.04547948132178276 0.3116388387808845 >10 0.2924315032160157 4.146200936641075 >50 0.02544955450213382 1.3505733123196224 >100 0.02438938869234824 3.909590208238682 >500 0.0030363958292274652 1.6463063097357487 >1k 0.0027425510715602913 4.245301653122258 >5k 9.794825255572468E-5 0.40919019394455763 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5487 0.4117610420173365 No Hit CTGTAGGACGTGGAATATGGCAAGA 4880 0.3662099298422821 No Hit GTCCTACAGTGGACATTTCTAAATT 4129 0.309852623016144 No Hit CTTTAGGACGTGAAATATGGCGAGG 4101 0.3077514185006555 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3917 0.29394350311315964 No Hit TATCAACGCAGAGTACTTTTTTTTT 3899 0.29259272878177417 No Hit GGTATCAACGCAGAGTACTTTTTTT 3860 0.2896660510637723 No Hit CTGAAGGACCTGGAATATGGCGAGA 2379 0.17852734079811253 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2023 0.15181202624404438 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2020 0.1515868971888135 No Hit ATTTAGAAATGTCCACTGTAGGACG 1778 0.13342648673351998 No Hit GAATATGGCAAGAAAACTGAAAATC 1733 0.13004955090505632 No Hit CTGTAGGACCTGGAATATGGCGAGA 1657 0.12434628150587324 No Hit GGAATATGGCGAGAAAACTGAAAAT 1655 0.12419619546905263 No Hit TTGTAGAACAGTGTATATCAATGAG 1602 0.12021891549330653 No Hit GTCCTACAGTGTGCATTTCTCATTT 1536 0.11526607627822649 No Hit ACAGTGGACATTTCTAAATTTTCCA 1464 0.10986297895268463 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1398 0.10491013973760459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.504301841030371E-5 0.0 0.0 0.0 0.0 7 7.504301841030371E-5 0.0 0.0 0.0 0.0 8 7.504301841030371E-5 0.0 0.0 0.0 0.0 9 7.504301841030371E-5 0.0 0.0 0.0 0.0 10 7.504301841030371E-5 0.0 0.0 0.0 0.0 11 7.504301841030371E-5 7.504301841030371E-5 0.0 0.0 0.0 12 7.504301841030371E-5 7.504301841030371E-5 0.0 0.0 0.0 13 7.504301841030371E-5 7.504301841030371E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 200 0.0 14.246623 18 TAGGACC 770 0.0 13.200151 4 CCAACGA 120 7.421477E-10 12.664615 19 CACCTTT 690 0.0 12.388368 14 ACCTTTT 745 0.0 12.366197 15 CCACCTT 640 0.0 12.317393 13 GGTATCA 1180 0.0 12.038997 1 CCAACGT 105 2.724264E-7 11.76 19 ATCCCGT 130 2.6229827E-9 11.689537 10 AGGACCT 1595 0.0 11.494298 5 GGACCTG 1530 0.0 11.484205 6 CCCGTTT 135 4.7402864E-9 11.256591 12 TCCCGTT 135 4.7402864E-9 11.256591 11 CCTTTTT 795 0.0 11.230043 16 AATCCCG 280 0.0 11.194615 19 TTCCACC 675 0.0 11.115884 11 AAATGTC 670 0.0 11.05708 7 AATGTCC 655 0.0 11.020289 8 AGGACGG 130 3.2674507E-8 10.960587 5 TCCACCT 730 0.0 10.928917 12 >>END_MODULE