FastQCFastQC Report
Thu 26 May 2016
SRR1512239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835534
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT88670.3127100574353896No Hit
CTGTAGGACGTGGAATATGGCAAGA77140.27204752261831455No Hit
GTCCTAAAGTGTGTATTTCTCATTT71790.25317982432938557No Hit
CTTTAGGACGTGAAATATGGCGAGG64270.2266592465475639No Hit
GTATCAACGCAGAGTACTTTTTTTT44050.15534992703314437No Hit
GTCCTACAGTGTGCATTTCTCATTT36960.13034581845959173No Hit
CTGAAGGACCTGGAATATGGCGAGA33660.11870779895427105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3150.013.15855518
CGGTAGG903.2296684E-612.5163321
CCAACGA2300.012.28738619
TAGGACC13100.011.2892164
GGTATCA14800.010.93256951
GGCGAGG21150.010.91243819
TGTAGGA43550.010.8872882
AATCCCG5600.010.7660919
GTCCTAC41650.010.6709141
CTGTAGG43400.010.6417661
TAGACGG804.080588E-410.5971235
TCCTACA44400.010.3098282
GTAGGAC45250.010.3043843
GTATAAA4900.010.2406351
GGACGTG47100.010.1996366
CACCTTT10600.010.13111914
AGGACGT48300.010.1217525
GTGTAGG4150.010.1172521
AGGACCT28450.010.0984115
ACCTTTT10450.010.096251515
Produced by FastQC (version 0.11.5)