FastQCFastQC Report
Thu 26 May 2016
SRR1512239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835534
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT101310.35728719881334525No Hit
CTGTAGGACGTGGAATATGGCAAGA88930.3136269923055058No Hit
GTCCTAAAGTGTGTATTTCTCATTT82840.29214955630932304No Hit
CTTTAGGACGTGAAATATGGCGAGG78360.2763500631627059No Hit
GTATCAACGCAGAGTACTTTTTTTT57920.20426487568126497No Hit
GTCCTACAGTGTGCATTTCTCATTT43070.15189378790732186No Hit
CTGAAGGACCTGGAATATGGCGAGA41170.14519311001031904No Hit
GGTATCAACGCAGAGTACTTTTTTT35720.12597274446365306No Hit
TATCAACGCAGAGTACTTTTTTTTT34950.12325720657907822No Hit
CTGTAGGACCTGGAATATGGCGAGA34690.12234027170896206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13700.013.7295964
TATTGCG905.429192E-712.66301116
TACGGCG658.046465E-411.68893316
GGTATCA16450.011.539141
TATGTCG2100.011.3062616
TCCAACG3450.011.28659618
AATCCCG5600.011.19418419
GTCCTAG5650.011.1424761
AGGACGT47500.011.0997325
GTCGAGG1803.6379788E-1211.08111119
GGACGTG47150.010.95766
CTGTAGG46200.010.942591
TGTAGGA46000.010.9040842
GATATAC10500.010.8104531
TAGAAAT11150.010.7351674
GTAGGAC46400.010.7281893
GGCGAGG19800.010.6973519
AAATGTC9900.010.648447
GACGTGG23900.010.6099077
TACACCG909.522839E-510.5553015
Produced by FastQC (version 0.11.5)