FastQCFastQC Report
Thu 26 May 2016
SRR1512238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604307
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT59790.22958122832676792No Hit
CTGTAGGACGTGGAATATGGCAAGA52810.20277947261977944No Hit
GTCCTAAAGTGTGTATTTCTCATTT48330.1855771996158671No Hit
CTTTAGGACGTGAAATATGGCGAGG45420.17440340174948651No Hit
GTATCAACGCAGAGTACTTTTTTTT42130.16177048251223838No Hit
GGTATCAACGCAGAGTACTTTTTTT26090.10018020148930215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG903.2774133E-612.4998261
TAGGACC9300.012.4603784
GGACGTA803.1229854E-511.7765766
GGTATCA12950.011.6091751
CGACTGT658.6068426E-411.5953976
TCCAACG2550.011.45351718
GGCGAGG14300.010.93677819
TCCGCAC700.00159921710.7671553
GGATCGT700.00159921710.7671556
AGGACCT20100.010.7336755
GTCCTAC28550.010.7107251
TGTAGGA32500.010.6047482
CAAGTCG2700.010.4680676
AGGACGT34050.010.32055
CTGTAGG31900.010.291251
AAGTCGT2750.010.2777397
GTCCTAA16450.010.2582171
GTCAACG905.66197E-410.2271312
ATTTCTA23350.010.20804715
ATTTAGA8350.010.1658911
Produced by FastQC (version 0.11.5)