FastQCFastQC Report
Thu 26 May 2016
SRR1512238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604307
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69000.26494572260490024No Hit
CTGTAGGACGTGGAATATGGCAAGA62660.2406014344698993No Hit
GTATCAACGCAGAGTACTTTTTTTT57310.22005854148531645No Hit
GTCCTAAAGTGTGTATTTCTCATTT55510.21314691393910165No Hit
CTTTAGGACGTGAAATATGGCGAGG51860.19913166919261052No Hit
TATCAACGCAGAGTACTTTTTTTTT37010.14211074193633855No Hit
GGTATCAACGCAGAGTACTTTTTTT36720.1409972019427817No Hit
GTCCTACAGTGTGCATTTCTCATTT28560.10966449039994132No Hit
CTGAAGGACCTGGAATATGGCGAGA27970.10739901248201537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2550.014.15277118
AGCGCAC550.003076054412.08741510
TAGGACC9450.011.9627214
GGTATCA15650.011.5802371
TTAAACG700.0014922410.8569234
GGCGAGG14500.010.74275419
AGGACGT31250.010.7005845
GACGTGG15550.010.688237
CCGTGCA803.7826083E-410.6844119
GGACGTG30850.010.6517036
CCAACGA2150.010.60260419
GTCCTAC31600.010.2930831
AATGTCC8850.010.1947938
GACGTGA16700.010.1228227
TGTAGGA33200.010.0434722
TAGGACT4300.09.941664
TTAGGAC19250.09.919283
CTGTAGG32200.09.8643061
AGGACCT21000.09.8617065
CCAACGT1554.07872E-89.80455919
Produced by FastQC (version 0.11.5)