FastQCFastQC Report
Thu 26 May 2016
SRR1512237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838251
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT62800.22126302430616604No Hit
CTGTAGGACGTGGAATATGGCAAGA54230.1910683727408182No Hit
GTCCTAAAGTGTGTATTTCTCATTT48590.17119698011204787No Hit
GTATCAACGCAGAGTACTTTTTTTT44430.15654006640004708No Hit
CTTTAGGACGTGAAATATGGCGAGG43070.15174838307112373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGA653.6624624E-614.5004636
TAGGACC9600.013.2543294
GGACGTA655.8748446E-513.0504166
CCAACGA2200.012.85290919
TCCAACG3250.012.76063218
GGCGAGG13600.011.64322319
CGGTTTC2050.011.49447213
CGGTAGG1258.5612555E-811.4078951
CCGGTTT2050.011.03469212
CGTTAGG650.006348314710.9691291
GTCCTAC28800.010.9636891
AGGACCT20050.010.7180475
GGTATCA15200.010.6546951
TGTAGGA31000.010.5141892
TTAGGAC18300.010.5068923
GACGTGA15950.010.3412397
GGACGTG30600.010.2261436
ATCCCGT2400.010.21074310
GATATAC9300.010.185621
AGGACGT31950.010.1775555
Produced by FastQC (version 0.11.5)