FastQCFastQC Report
Thu 26 May 2016
SRR1512237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838251
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT63940.2252795823907047No Hit
CTGTAGGACGTGGAATATGGCAAGA61610.2170703013933581No Hit
GTATCAACGCAGAGTACTTTTTTTT58110.20473876341451125No Hit
CTTTAGGACGTGAAATATGGCGAGG48930.1723949009442787No Hit
GTCCTAAAGTGTGTATTTCTCATTT48140.1696114966576247No Hit
TATCAACGCAGAGTACTTTTTTTTT39430.13892358357312304No Hit
GGTATCAACGCAGAGTACTTTTTTT37450.1319474563736611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8900.012.5959024
TATACCG855.3245083E-511.1768245
TCCAACG2750.011.05138918
TTAGGAC19750.011.0155373
GACGTGA17100.010.885787
GCCGGTT1855.456968E-1210.78070411
GTAGAAC11250.010.7247823
AGGACGT32750.010.5301035
GGCGAGG16350.010.51418719
GGACGTG32800.010.5107176
TGTAGAA12600.010.480492
ACCTTTT8650.010.32075115
GATATAC10050.010.1533941
GTCCGCT750.002655007210.1304411
AATCCCG3100.010.11037219
TAGACTG4050.010.0867375
AACCGTG856.61386E-410.0559517
TAGGACG34600.010.049454
GGTATCA18450.010.0276441
TGGCGAG31200.09.95388818
Produced by FastQC (version 0.11.5)