##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512237_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2838251 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.438059213226737 31.0 31.0 34.0 27.0 34.0 2 30.643598469620905 31.0 31.0 34.0 27.0 34.0 3 30.722463235281165 31.0 31.0 34.0 27.0 34.0 4 34.34720643100275 37.0 35.0 37.0 30.0 37.0 5 33.90587160895918 37.0 35.0 37.0 30.0 37.0 6 33.82194879874965 37.0 35.0 37.0 29.0 37.0 7 33.623736942222514 37.0 35.0 37.0 28.0 37.0 8 33.5075153677388 37.0 35.0 37.0 28.0 37.0 9 34.939121310976375 38.0 35.0 39.0 28.0 39.0 10 34.71161817612325 38.0 35.0 39.0 27.0 39.0 11 34.70409910892307 38.0 35.0 39.0 27.0 39.0 12 34.49044252957191 38.0 35.0 39.0 27.0 39.0 13 34.476664678352975 38.0 35.0 39.0 27.0 39.0 14 35.392567464963456 39.0 35.0 40.0 27.0 41.0 15 35.32171502802254 39.0 35.0 40.0 27.0 41.0 16 35.20283230764298 39.0 34.0 40.0 27.0 41.0 17 35.1144017918077 39.0 34.0 40.0 26.0 41.0 18 35.03033734507625 38.0 34.0 40.0 26.0 41.0 19 35.05156414989372 39.0 34.0 40.0 26.0 41.0 20 34.906766878616445 39.0 34.0 40.0 25.0 41.0 21 34.77913405121675 39.0 34.0 40.0 25.0 41.0 22 34.87201695692171 39.0 34.0 40.0 25.0 41.0 23 34.749226195991824 39.0 34.0 40.0 25.0 41.0 24 34.60250767109745 39.0 34.0 40.0 24.0 41.0 25 34.12459821206792 38.0 34.0 40.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1782224774897152 1101 2 0.20911859982521364 1101 3 0.236790227817675 1101 4 0.29044127810944786 1101 5 0.42886336505885936 1101 6 0.30534057218329735 1101 7 0.255030879652935 1101 8 0.13977730064043925 1101 9 0.2114759031503155 1101 10 0.2695909699503787 1101 11 0.2848338804186312 1101 12 0.38389544618937776 1101 13 0.17601194171407997 1101 14 0.3009770291636613 1101 15 0.2107591292590527 1101 16 0.22634179408517952 1101 17 0.20874931410327235 1101 18 0.26565615664330977 1101 19 0.17376232825069593 1101 20 0.2973257741592761 1101 21 -0.027454965806121834 1101 22 -0.06307319992129123 1101 23 -0.20166114743350505 1101 24 -0.11036625092061314 1101 25 -0.12069675968781723 1102 1 0.3871563206377715 1102 2 0.43172233435433327 1102 3 0.3599392087620217 1102 4 0.3629761037338781 1102 5 0.354659071942649 1102 6 0.5424762318152645 1102 7 0.4910124092692385 1102 8 0.46929043044954 1102 9 0.6670455285785053 1102 10 0.5712366071183652 1102 11 0.5390528697977288 1102 12 0.6204423408238426 1102 13 0.8059867235294433 1102 14 0.775341679379693 1102 15 0.8935490092790204 1102 16 0.8549679839875779 1102 17 0.9392662761296791 1102 18 1.0369175930470078 1102 19 0.9225947499710401 1102 20 1.104990316358851 1102 21 1.071601181233973 1102 22 0.8915566608192478 1102 23 0.9869970170613556 1102 24 1.1441421450995648 1102 25 1.025701849008584 1103 1 0.3269794596322093 1103 2 0.2321612909606614 1103 3 0.25453116151871313 1103 4 0.3981888992124212 1103 5 0.21048674444890736 1103 6 0.4193323265928939 1103 7 0.4495965650507685 1103 8 0.56446741617345 1103 9 0.6720345221846102 1103 10 0.6350341975599534 1103 11 0.5108475563805257 1103 12 0.5720815036311677 1103 13 0.573084569227909 1103 14 0.48630340851639176 1103 15 0.6346675041780401 1103 16 0.6243255701887449 1103 17 0.4777615025350457 1103 18 0.5837011329277786 1103 19 0.7167708969640856 1103 20 0.5859980509748439 1103 21 0.829550676540407 1103 22 0.9367010638756739 1103 23 1.1022915913976448 1103 24 0.834672541038735 1103 25 0.9353593880246436 1104 1 0.39859276243235797 1104 2 0.4228428791860068 1104 3 0.4657741616574711 1104 4 0.4541886753789157 1104 5 0.5380868339823124 1104 6 0.5657433689784526 1104 7 0.6012304737003618 1104 8 0.6105567358715049 1104 9 0.8277136391259745 1104 10 0.7992489926510089 1104 11 0.8944266592436705 1104 12 0.9018076316862178 1104 13 0.8970335645598126 1104 14 1.0792000116150149 1104 15 1.161769083065174 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23 -0.7388095560197385 2314 24 -0.6423795743847123 2314 25 -0.795520963980934 2315 1 -0.241725462307965 2315 2 -0.29143851835884504 2315 3 -0.2714231943387517 2315 4 -0.3272419195219385 2315 5 -0.5013409280573526 2315 6 -0.464379388339772 2315 7 -0.4568790635989757 2315 8 -0.34115336800007157 2315 9 -0.3206385799486213 2315 10 -0.34716649365682883 2315 11 -0.46028627748909656 2315 12 -0.5348968064629815 2315 13 -0.5841760671682295 2315 14 -0.8593259562792213 2315 15 -0.5891525411085752 2315 16 -0.7484060470201754 2315 17 -0.7005970959633459 2315 18 -0.8277810503638463 2315 19 -0.7558345135948485 2315 20 -0.785817823176032 2315 21 -0.7167379660528539 2315 22 -0.6049123314288138 2315 23 -0.7379157736363169 2315 24 -0.6402241339702002 2315 25 -0.6769173370379278 2316 1 -0.9944335516595153 2316 2 -0.8892598152575673 2316 3 -0.953456809606589 2316 4 -0.9689920821764559 2316 5 -1.125985039963659 2316 6 -1.222192227203358 2316 7 -1.258380473271501 2316 8 -1.0906775493056458 2316 9 -1.3451625443815018 2316 10 -1.4664538499786701 2316 11 -1.3672340111694226 2316 12 -1.6120481836045997 2316 13 -1.5053094438761079 2316 14 -1.9111377107793288 2316 15 -1.8436192511497822 2316 16 -1.7430252185664727 2316 17 -1.9951000237447403 2316 18 -1.881785170945065 2316 19 -1.7744699505933283 2316 20 -1.7936584217446665 2316 21 -1.7664521278398837 2316 22 -1.9108247148597428 2316 23 -1.7087941080540148 2316 24 -1.6689124879064394 2316 25 -1.5737956714556205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55398.0 3 1.0 4 989.0 5 8784.0 6 11112.0 7 8825.0 8 7149.0 9 6474.0 10 5903.0 11 5875.0 12 5575.0 13 5218.0 14 5155.0 15 5336.0 16 5445.0 17 5699.0 18 6187.0 19 6842.0 20 8344.0 21 10700.0 22 13839.0 23 17235.0 24 20958.0 25 25144.0 26 29157.0 27 33380.0 28 38870.0 29 47339.0 30 60873.0 31 77410.0 32 98868.0 33 127331.0 34 163484.0 35 215877.0 36 309120.0 37 514314.0 38 870041.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.154061957616136 20.060800058868438 14.123189282547985 24.661948700967436 2 15.001738746855017 20.812852704006797 40.55155798412473 23.633850565013454 3 19.075303769821623 24.561428851782313 30.176788451761315 26.18647892663475 4 13.125195763165415 17.45007753014092 37.61552449025826 31.809202216435406 5 13.300477159331885 37.86965398459232 35.74546850180992 13.084400354265869 6 32.179712083251275 37.33496438475667 17.58142602609847 12.903897505893594 7 28.463374099049027 31.47832943598012 21.811566348430777 18.246730116540082 8 25.01043776607495 37.96334432719305 20.064962542072564 16.961255364659433 9 26.043186455320548 15.706116196206748 20.270828760388 37.979868588084706 10 15.095528901425562 29.591568892250898 33.97610007007837 21.336802136245172 11 34.606911087144866 22.389457451085192 24.48419819106908 18.519433270700862 12 23.48403999505329 24.542508749226197 31.59329460290862 20.38015665281189 13 29.09781411157787 21.907858043562744 25.851871451820152 23.142456393039236 14 20.74562820553926 22.446358690616158 27.388522015847084 29.4194910879975 15 22.835823892953794 31.548231639837347 23.137100982259852 22.478843484949003 16 22.011760059275943 28.489763590323758 27.877027084637685 21.621449265762614 17 21.594002785518267 28.938455407925517 27.919447575284924 21.5480942312713 18 21.388171800168486 27.775626609485915 30.074824249159075 20.761377341186527 19 23.556091409815412 26.984716996488327 27.44588128393155 22.013310309764712 20 23.506166297483908 27.699840500364488 28.06714416730585 20.726849034845753 21 22.88923706888503 27.00356663311314 27.18413558208911 22.923060715912722 22 23.06744540916219 28.153359234260815 27.493939049083398 21.285256307493594 23 22.264239491151415 27.866034399353683 27.797118718534758 22.072607390960137 24 23.116524930318004 26.911819990550516 28.483421656506064 21.488233422625413 25 22.725237089354895 27.608176697855757 28.105452130236625 21.561134082552723 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 220.0 1 220.0 2 372.0 3 524.0 4 524.0 5 524.0 6 1322.0 7 2120.0 8 2120.0 9 2120.0 10 3358.0 11 4596.0 12 4596.0 13 4596.0 14 8018.0 15 11440.0 16 11440.0 17 11440.0 18 21008.5 19 30577.0 20 30577.0 21 30577.0 22 50376.0 23 70175.0 24 70175.0 25 70175.0 26 103163.5 27 136152.0 28 136152.0 29 136152.0 30 179105.5 31 222059.0 32 222059.0 33 222059.0 34 250316.0 35 278573.0 36 278573.0 37 278573.0 38 299590.0 39 320607.0 40 320607.0 41 320607.0 42 340470.0 43 360333.0 44 360333.0 45 360333.0 46 365972.5 47 371612.0 48 371612.0 49 371612.0 50 357219.5 51 342827.0 52 342827.0 53 342827.0 54 304671.0 55 266515.0 56 266515.0 57 266515.0 58 227623.5 59 188732.0 60 188732.0 61 188732.0 62 154097.0 63 119462.0 64 119462.0 65 119462.0 66 91768.0 67 64074.0 68 64074.0 69 64074.0 70 46974.0 71 29874.0 72 29874.0 73 29874.0 74 20873.0 75 11872.0 76 11872.0 77 11872.0 78 8031.5 79 4191.0 80 4191.0 81 4191.0 82 2741.0 83 1291.0 84 1291.0 85 1291.0 86 819.5 87 348.0 88 348.0 89 348.0 90 208.0 91 68.0 92 68.0 93 68.0 94 38.0 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4090194982755225 2 0.0 3 0.0 4 0.0 5 0.029384293355309305 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006553331611615745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2838251.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.42872512107962 #Duplication Level Percentage of deduplicated Percentage of total 1 84.59432739186131 60.42464958075878 2 10.470850966508834 14.95839070941101 3 2.4707944262511763 5.2945708771017275 4 0.9533488890789461 2.723859829700267 5 0.4534429305655843 1.619442522273296 6 0.2650899663064592 1.1361023001396202 7 0.17105578935256754 0.8552807870623693 8 0.1162467296802396 0.6642684560443426 9 0.08365641004508015 0.5377923645953719 >10 0.38119055064120366 4.604726969959764 >50 0.021154336541502356 1.056730889746496 >100 0.014997224136498788 2.1826917356172757 >500 0.0017743333990133021 0.8607295756213492 >1k 0.0019221945149904113 2.434186471132023 >5k 1.4786111653772394E-4 0.6465769308362486 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6394 0.2252795823907047 No Hit CTGTAGGACGTGGAATATGGCAAGA 6161 0.2170703013933581 No Hit GTATCAACGCAGAGTACTTTTTTTT 5811 0.20473876341451125 No Hit CTTTAGGACGTGAAATATGGCGAGG 4893 0.1723949009442787 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4814 0.1696114966576247 No Hit TATCAACGCAGAGTACTTTTTTTTT 3943 0.13892358357312304 No Hit GGTATCAACGCAGAGTACTTTTTTT 3745 0.1319474563736611 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.5232965653848094E-5 2 0.0 0.0 0.0 0.0 3.5232965653848094E-5 3 0.0 0.0 0.0 0.0 3.5232965653848094E-5 4 0.0 0.0 0.0 0.0 3.5232965653848094E-5 5 0.0 0.0 0.0 0.0 3.5232965653848094E-5 6 7.046593130769619E-5 0.0 0.0 0.0 7.046593130769619E-5 7 7.046593130769619E-5 0.0 0.0 0.0 7.046593130769619E-5 8 7.046593130769619E-5 0.0 0.0 0.0 7.046593130769619E-5 9 7.046593130769619E-5 0.0 0.0 3.5232965653848094E-5 7.046593130769619E-5 10 1.0569889696154428E-4 0.0 0.0 3.5232965653848094E-5 7.046593130769619E-5 11 1.0569889696154428E-4 0.0 0.0 3.5232965653848094E-5 7.046593130769619E-5 12 1.0569889696154428E-4 0.0 0.0 7.046593130769619E-5 7.046593130769619E-5 13 1.0569889696154428E-4 0.0 0.0 7.046593130769619E-5 7.046593130769619E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 890 0.0 12.595902 4 TATACCG 85 5.3245083E-5 11.176824 5 TCCAACG 275 0.0 11.051389 18 TTAGGAC 1975 0.0 11.015537 3 GACGTGA 1710 0.0 10.88578 7 GCCGGTT 185 5.456968E-12 10.780704 11 GTAGAAC 1125 0.0 10.724782 3 AGGACGT 3275 0.0 10.530103 5 GGCGAGG 1635 0.0 10.514187 19 GGACGTG 3280 0.0 10.510717 6 TGTAGAA 1260 0.0 10.48049 2 ACCTTTT 865 0.0 10.320751 15 GATATAC 1005 0.0 10.153394 1 GTCCGCT 75 0.0026550072 10.13044 11 AATCCCG 310 0.0 10.110372 19 TAGACTG 405 0.0 10.086737 5 AACCGTG 85 6.61386E-4 10.055951 7 TAGGACG 3460 0.0 10.04945 4 GGTATCA 1845 0.0 10.027644 1 TGGCGAG 3120 0.0 9.953888 18 >>END_MODULE