FastQCFastQC Report
Thu 26 May 2016
SRR1512236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885129
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT85480.2962779133965933No Hit
CTGTAGGACGTGGAATATGGCAAGA75560.2618947021086406No Hit
GTCCTAAAGTGTGTATTTCTCATTT69100.23950402217717126No Hit
CTTTAGGACGTGAAATATGGCGAGG60380.20928007031921275No Hit
GTATCAACGCAGAGTACTTTTTTTT52860.18321537789124853No Hit
GTCCTACAGTGTGCATTTCTCATTT35310.12238620872758203No Hit
GGTATCAACGCAGAGTACTTTTTTT32540.11278525154334519No Hit
TATCAACGCAGAGTACTTTTTTTTT31290.10845268963710114No Hit
CTGAAGGACCTGGAATATGGCGAGA30540.10585315249335471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1103.6379788E-1215.7622421
TACCGTC400.00557022714.1357187
TAGGACC10950.012.8232694
GCGTATA708.238202E-411.6561111
GACTGCG908.166522E-611.5179917
TTCAACG801.9538657E-411.4739862
TCCAACG4250.011.30955318
TGTAGGA41600.011.0571952
AGTACCG600.006240640310.9944475
GGTATCA16700.010.87089351
AGGACGT43500.010.8319685
ACTCCGT700.001595701710.770076
GTCTTAG3350.010.6557731
TTAGGAC25750.010.6498223
CTGTAGG41400.010.6179021
GGACGTG43400.010.5746456
GGCGAGG18850.010.54958419
CCAACGA2950.010.54280519
GTAGGAC41800.010.4383373
GACGTGG22600.010.2577777
Produced by FastQC (version 0.11.5)